Acute exercise during hemodialysis prevents the decrease in natural killer cells in patients with chronic kidney disease: a pilot study

  • Maria Isabel Fuhro
  • Gilson P. Dorneles
  • Francini P. Andrade
  • Pedro R. T. Romão
  • Alessandra Peres
  • Mariane B. Monteiro
Nephrology - Original Paper

Abstract

Purpose

To evaluate the acute response of natural killer (NK) cell subsets of chronic kidney disease patients submitted to intradialytic exercise in a randomized crossover study.

Methods

Nine patients were submitted to a single bout of 20-min intradialytic exercise and a control hemodialysis (HD) session with an interval of 7 days between them. Peripheral blood sample was collected at baseline, during HD and immediately after HD in each trial to evaluate the peripheral frequency of NK cells and their subsets (CD3-CD56bright and CD3-CD56dim), systemic cortisol concentrations, C-reactive protein (CRP), creatine kinase activity (CK), and urea and creatinine levels.

Results

HD therapy induced a significant decrease in NK cells frequency (p = 0.039), NK CD3-CD56bright cells (p = 0.04), and CD3-CD56dim cells (p = 0.036). On the other hand, no significant alterations were observed in NK cells and NK subsets during and after intradialytic exercise trial (p > 0.05). Neither trial altered CRP levels or serum CK activity during and after HD therapy (p > 0.05). However, HD therapy increased cortisol concentrations after HD therapy (p = 0.034).

Conclusions

This study suggests the potential role of intradialytic exercise to prevent the decrease in peripheral frequency of NK cell subsets during HD therapy. Moreover, moderate intensity intradialytic exercise did not exacerbate the systemic inflammation or induce muscle damage during HD therapy.

Keywords

Inflammation Hemodialysis Exercise NK cells Immune response 

Notes

Compliance with ethical standards

Conflict of interest

The authors declared they have no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The study protocol was approved by the Ethics Committee of the Methodist University Center IPA. This study was registered in Registro Brasileiro de Ensaios Clínicos (U1111-1172-9511).

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  1. 1.Research CenterMethodist University Center IPAPorto AlegreBrazil
  2. 2.Graduate Program in Health Sciences, Laboratory of Cellular and Molecular ImmunologyFederal University of Health Sciences of Porto Alegre (UFCSPA)Porto AlegreBrazil
  3. 3.Federal University of Rio Grande do SulPorto AlegreBrazil
  4. 4.Physical Therapy DepartmentFederal University of Health Sciences of Porto Alegre (UFCSPA)Porto AlegreBrazil

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