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Decreased percentage of peripheral naïve T cells is independently associated with ischemic stroke in patients on hemodialysis

  • Nephrology – Original Paper
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Abstract

Purpose

Cerebrovascular complications, including ischemic stroke, account for poor outcomes in patients on hemodialysis. T cell responses may be involved in the pathogenesis of ischemic stroke. We aimed to evaluate the role of naïve T cells in development of ischemic stroke in patients on hemodialysis.

Methods

In this cross-sectional study, 156 patients on hemodialysis in our blood purification center were included. These patients were divided into the ischemic stroke (IS) group (61 cases) and non-ischemic stroke (non-IS) group (95 cases) according to a new diagnosis after initiation of hemodialysis. After being lysed with red blood cell lysis solution, peripheral blood was tested by flow cytometry to detect the expression of CD45RO and CCR7 in CD4 T and CD8 T cells. Correlation analysis and logistic regression analysis were conducted to identify potential independent risk factors for ischemic stroke.

Results

The percentage of peripheral naïve T cells was lower in the IS group [median (interquartile range (IQR)) 13.9% (8.6–22.9%)] compared with the non-IS group [median (IQR) 22.7% (15.9–32.2%), P < 0.001]. Spearman correlation analysis showed that naïve T cells were negatively associated with ischemic stroke (r = −0.308, P < 0.001). Multivariate logistic regression analysis showed that CD4 naïve T cells had an independent negative association with ischemic stroke in patients on hemodialysis (odds ratio 0.933, 95% CI 0.883, 0.986; P = 0.013).

Conclusion

A decrease in percentage of peripheral CD4 naïve T cells is a risk factor for ischemic stroke in patients on hemodialysis.

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Acknowledgements

We acknowledge the precious help provided by the Yubin Zhang from the School of Public Health Fudan University. We thank Ellen Knapp, Ph.D., from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript. This study was founded by the Shanghai Committee of Science and Technology, China (Grant No. 15DZ0503402), and the National Natural Science Funds of China (No. 81430015).

Author information

Author notes

  1. Rongyi Chen and Jiachang Hu have contributed equally to this work.

Authors and Affiliations

  1. Division of Nephrology, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, 200032, China

    Rongyi Chen, Jiachang Hu, Fangfang Xiang, Xiao Tan, Bo Shen, Zhonghua Liu, Wenlv Lv, Xiaoqiang Ding, Xuesen Cao & Jianzhou Zou

  2. Shanghai Institute of Kidney and Dialysis, Shanghai, 200032, China

    Rongyi Chen, Jiachang Hu, Fangfang Xiang, Xiao Tan, Bo Shen, Zhonghua Liu, Wenlv Lv, Xiaoqiang Ding, Xuesen Cao & Jianzhou Zou

  3. Shanghai Key Lab for Kidney and Blood Purification, Shanghai, 200032, China

    Rongyi Chen, Jiachang Hu, Fangfang Xiang, Xiao Tan, Bo Shen, Zhonghua Liu, Wenlv Lv, Xiaoqiang Ding, Xuesen Cao & Jianzhou Zou

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  1. Rongyi Chen
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Correspondence to Xuesen Cao or Jianzhou Zou.

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All authors have no conflicts of interest, and there are no relationships with industry.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Chen, R., Hu, J., Xiang, F. et al. Decreased percentage of peripheral naïve T cells is independently associated with ischemic stroke in patients on hemodialysis. Int Urol Nephrol 49, 2051–2060 (2017). https://doi.org/10.1007/s11255-017-1691-y

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  • DOI: https://doi.org/10.1007/s11255-017-1691-y

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