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Assessment of the relationship between serum paraoxonase activity and epicardial adipose tissue in hemodialysis patients

  • Nephrology - Original Paper
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Abstract

Purpose

Paraoxonase-1 (PON-1) is a high-density lipoprotein-associated (HDL) enzyme, which has been shown to reduce susceptibility to low-density lipoprotein (LDL) peroxidation. Epicardial adipose tissue (EAT) is a marker of atherosclerosis. The aim of this study was to determine the relationship between PON-1 activity and EAT in hemodialysis (HD) patients.

Methods

This is a cross-sectional study conducted on 72 (43 males) HD patients with end-stage renal disease. Serum levels for lipid profiles, C-reactive protein, calcium, phosphate, and parathyroid hormone were measured. PON-1 activity was also measured and compared to the rate of enzymatic hydrolysis of paraoxon to p-nitrophenol. Echocardiography was used to measure EAT thickness (EATT). The correlation between PON-1 and EATT was assessed, while independent predictors of EATT in HD patients were similarly assessed using multivariate regression analysis.

Results

There was a significant low mean value of PON-1 activity in HD patients compared with the control group (82.1 ± 31.6 vs. 164.3 ± 61.5 U/l, p = 0.0001) and significant high mean value of EATT in HD patients, compared with controls (6.2 ± 1.7 vs. 3.9 ± 1.1 mm, p = 0.0001). In addition, there was a significant negative correlation between PON-1 activity and EATT (r = −0.484, p = 0.0001) and a significant positive correlation between PON-1 activity and HDL-C (r = 0.417, p = 0.0003). PON-1, total cholesterol, triglycerides, LDL, HDL, age, and body mass index were found to be independent predictors of EATT.

Conclusion

Our study demonstrated that PON-1 activity was significantly lower in HD patients compared with healthy controls and that PON-1 activity was inversely correlated with EATT in this population.

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Acknowledgements

Many thanks to Dr. Amna Metwaly, professor of cardiology, Intensive Care Unit, Theodor Bilharz Research Institute, Cairo, Egypt, for her help in study concept and design, acquisition of echocardiographic data, analysis and interpretation of data.

Author information

Authors and Affiliations

  1. Department of Nephrology, Theodor Bilharz Research Institute, El-Nile St. Waraq El-Hadar, P.O. Box 30, Imbaba, Giza, 12411, Egypt

    Emad Abdallah, Samya El-Shishtawy & Nevine Sherif

  2. Department of Intensive Care Unit, Theodor Bilharz Research Institute, Cairo, Egypt

    Ahmed Ali

  3. Department of Clinical Chemistry, Theodor Bilharz Research Institute, Cairo, Egypt

    Omnia El-Bendary

Authors
  1. Emad Abdallah
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  2. Samya El-Shishtawy
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  3. Nevine Sherif
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  4. Ahmed Ali
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  5. Omnia El-Bendary
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Corresponding author

Correspondence to Emad Abdallah.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was performed in accordance with the Declaration of Helsinki, and the study protocol was approved by the Institute Ethics Committee.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Abstract accepted as a poster presentation in ERA-EDTA, Vienna, Austria, May 21st–24th, 2016.

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Abdallah, E., El-Shishtawy, S., Sherif, N. et al. Assessment of the relationship between serum paraoxonase activity and epicardial adipose tissue in hemodialysis patients. Int Urol Nephrol 49, 329–335 (2017). https://doi.org/10.1007/s11255-016-1465-y

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  • DOI: https://doi.org/10.1007/s11255-016-1465-y

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