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Effect of pH on the isolation of urinary exosome

International Urology and Nephrology volume 49, pages 165–169 (2017)Cite this article

Abstract

Purpose

Urinary exosome is an ideal noninvasive, low-cost source of kidney diseases biomarkers. However, many factors effect the isolation of urine-derived exosome. The effect of pH on the yield of exosome isolation under different pH was explored.

Methods

The pH was adjusted for 4, 5, 6, 7 and 8 with hydrochloric acid and sodium hydroxide solution. All samples were incubated for 30 min before differential ultracentrifugation. Exosome-associated protein markers TSG101, ALIX and total concentration of RNA were evaluated by Western-blotting and micro-amount ultraviolet spectrophotometer, respectively.

Results

A major loss of urinary exosome was received at pH 8 compared with alkali medium or control group. There was no difference whether or not added EDTA-Na2.

Conclusions

Acidic environment was likely to conducive to the isolation of exosome and maintain its stability and integrity that suggest pH medium needs to be carefully considered and also provide a methodology for future separation exosome.

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Author information

Affiliations

  1. Medical Technology Institute of Xuzhou Medical University, Xuzhou, 221004, China

    Yao Zhao

  2. Department of Clinical Laboratory, People’s Hospital of Guanyun, Lianyungang, 222001, China

    Kaiyong Chen

  3. Center of Research Laboratory, Lianyungang Hospital Affiliated to Xuzhou Medical College, 182 Tongguan Road, Lianyungang, 222001, China

    Haining Li & Huiyi Wu

Authors
  1. Yao Zhao
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  2. Kaiyong Chen
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  3. Haining Li
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  4. Huiyi Wu
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Correspondence to Huiyi Wu.

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The authors declare that they have no conflicts of interest that could inappropriately influence the content of this article.

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Cite this article

Zhao, Y., Chen, K., Li, H. et al. Effect of pH on the isolation of urinary exosome. Int Urol Nephrol 49, 165–169 (2017). https://doi.org/10.1007/s11255-016-1408-7

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  • DOI: https://doi.org/10.1007/s11255-016-1408-7

Keywords

  • pH
  • Urinary exosome
  • Proteins
  • EDTA-Na2
  • RNA