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Tripterysium glycosides preconditioning attenuates renal ischemia/reperfusion injury in a rat model

  • Urology - Original Paper
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An Urology – Letter to the Editor to this article was published on 31 March 2016

Abstract

Background

Ischemia–reperfusion (I/R) injury to the kidney occurs commonly in organ transplantation from donation after cardiac death, involving many pathologic processes. In this study, we used rat model to assess whether tripterysium glycosides (TG) preconditioning could exert protective effects in renal I/R injury.

Materials and methods

All male SD rats were randomly divided into four groups (6 each): sham group, TG group, I/R group and TG + I/R group. Groups TG and TG + I/R were pretreated with TG at 0.1 mg/kg for 14 days; groups sham and I/R were administered with the same dosage of normal saline. Groups TG + I/R and I/R underwent 45 min of renal ischemia of left kidney after right nephrectomy, and then, they were subjected to 72-h reperfusion. Groups sham and TG were only received right nephrectomy. The indicators of apoptosis, fibrosis and inflammation were analyzed to evaluate the effect of tripterysium glycosides preconditioning on renal I/R injury.

Results

Pretreatment with TG significantly inhibited the levels of serum creatine and blood urea nitrogen and improved histologic lesions induced by I/R injury. Moreover, for the apoptosis signal pathway, pretreatment with TG markedly decreased the expression of caspase-3 and Bax and increased the level of Bcl-2. HMGB1, which was regarded as one of inflammation marker molecule, it was inhibited in the TG + I/R group. For the fibrosis signal pathway, the pretreatment with TG before I/R could down-regulate the expression level of typical molecules of fibrosis (TGF-β1, Smad3, p-Smad3).

Conclusions

Pretreatment with tripterysium glycosides exhibited protective effect on kidney ischemia/reperfusion injury, which might be related to the alleviation of inflammation, fibrosis and the reduction in apoptosis.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81400753), Hubei Natural Science Foundation (No. 2014CFB362), the bureau of public health of Hubei province (JX6B14) and the project of Wuhan Municipal Science and Technology Bureau (2013062301010808).

Author’s contributions

Zhi-shun Wang carried out the design of the study. Tao Qiu participated in the design and helped to draft the manuscript. Zhong-bao Chen carried out the experiment. Xiu-heng Liu performed the statistical analysis. Jiang-qiao Zhou, Long Zhang, Ye Shen and Lu Zhang carried out some parts of the study. All authors read and approved the final manuscript.

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    Authors and Affiliations

    1. Department of Urology, Renmin Hospital of Wuhan University, Wuhan University, ZiYang Road 99#, Wuhan, 430060, China

      Zhi-shun Wang, Xiu-heng Liu, Lei Wang, Long Zhang, Ye Shen & Lu Zhang

    2. Department of Organ Transplantation, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, 430060, China

      Tao Qiu, Jiang-qiao Zhou & Zhong-bao Chen

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    1. Zhi-shun Wang
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    Correspondence to Xiu-heng Liu.

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    Zhi-shun Wang and Tao Qiu contributed equally to this work as co-first authors.

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    Wang, Zs., Qiu, T., Liu, Xh. et al. Tripterysium glycosides preconditioning attenuates renal ischemia/reperfusion injury in a rat model. Int Urol Nephrol 48, 213–221 (2016). https://doi.org/10.1007/s11255-015-1160-4

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    • DOI: https://doi.org/10.1007/s11255-015-1160-4

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