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The protective effect of MCP-1 -2518 A>G promoter polymorphism in Turkish chronic renal failure patients requiring long-term hemodialysis

  • Nephrology - Original Paper
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Abstract

Objective

Monocyte chemoattractant protein-1 (MCP-1) plays a major role in the pathogenesis and progression of different types of human renal disease. Therefore, in this study, we aimed to investigate the effect of MCP-1 gene -2518 A>G promoter polymorphism in chronic renal failure (CRF) patients requiring long-term hemodialysis.

Methods

The study population consisted of 201 adult CRF patients requiring long-term hemodialysis and 194 healthy controls. The polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) technique was used for genotyping of MCP-1 -2518 A>G polymorphism in the CRF patients and healthy controls.

Results

There were statistically significant differences in terms of genotypic (χ 2 = 12.69, p = 0.02) and allelic (χ 2 = 5.72, p = 0.02) frequencies of MCP-1 -2518 A>G between CRF patients and control subjects. According to our results, in the patient group MCP-1 -2518 AA genotype frequency was significantly higher than that of control group. On the other hand, heterozygous AG genotype frequency in the control group was significantly higher than that of the study group. Three different main disease subgroups of CRF (hypertension, diabetes mellitus, and atherosclerosis) patients were also evaluated, and significant associations were found between hypertension (genotype: χ 2 = 9.28, p = 0.01; allele: χ 2 = 6.00, p = 0.01), atherosclerosis (genotype: χ 2 = 5.37, p = 0.02; allele: χ 2 = 4.13, p = 0.04), and distributions of MCP-1 -2518 A>G genotypes and alleles. However, no significant association was found between diabetes mellitus and distributions of MCP-1 -2518 A>G genotype and allele frequencies (genotype: χ 2 = 2.37, p = 0.3; allele: χ 2 = 1.88, p = 0.17).

Conclusion

Current data show that MCP-1 -2518 AA genotype may cause susceptibility to CRF, while G allele may have a protective effect against development of CRF. In addition, MCP-1 -2518 AA genotype seems to associate with CRF originated from hypertension and atherosclerosis in our study population.

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Authors and Affiliations

  1. Department of Nutrition and Dietetics, Faculty of Health Sciences, Cumhuriyet University, Sivas, Turkey

    Binnur Bagci

  2. Department of Medical Genetics, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey

    Gokhan Bagci & Ilhan Sezgin

  3. Division of Nephrology, Department of Internal Medicine, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey

    Ferhan Candan

  4. Department of Medical Genetics, Faculty of Medicine, Canakkale On Sekiz Mart University, Canakkale, Turkey

    Ozturk Ozdemir

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  1. Binnur Bagci
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  2. Gokhan Bagci
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  5. Ilhan Sezgin
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Correspondence to Binnur Bagci.

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Bagci, B., Bagci, G., Candan, F. et al. The protective effect of MCP-1 -2518 A>G promoter polymorphism in Turkish chronic renal failure patients requiring long-term hemodialysis. Int Urol Nephrol 47, 551–556 (2015). https://doi.org/10.1007/s11255-015-0922-3

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  • DOI: https://doi.org/10.1007/s11255-015-0922-3

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