Abstract
Purpose
To investigate the relationship between the expression of LSD1 and E-cadherin in prostate cancer and their prognostic significance.
Methods
The expression of LSD1 and E-cadherin in prostate cancer was detected using immunohistochemistry, and the relationship between the expressions of these two molecules was analyzed by correlation analysis. Furthermore, LNCap cell line was treated with Pargyline (an inhibitor of LSD1), and Western blot was used to analyze LSD1 and E-cadherin expression.
Results
LSD1 expression increased significantly in prostate cancer specimens compared with benign prostatic hyperplasia (P < 0.05). Further analysis testified that LSD1 expression was positively correlated with higher Gleason Score, distant metastases, and poor prognosis (P < 0.05). Nevertheless, E-cadherin expression decreased significantly in prostate cancer specimens compared with benign prostatic hyperplasia (P < 0.05) and was negatively correlated with higher Gleason Score, distant metastases (P < 0.05). Correlation analysis revealed that LSD1 expression was negatively correlated with E-cadherin expression in prostate cancer (rs = −0.486, P = 0.001). Positive LSD1 expression and negative E-cadherin expression were significantly correlated with high 2-year progression (occurrence of castration-resistant prostate cancer) rate and low 5-year survival rate (P < 0.05). Moreover, Pargyline inhibited activity of LSD1 and up-regulated E-cadherin expression.
Conclusion
High LSD1 expression combined with low E-cadherin expression might be predictors of prostate cancer progression and metastasis. Inhibition of LSD1 may be a potential therapeutic target for prevention of prostate cancer.
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Acknowledgments
This study is supported by the Grants from the National Natural Science Foundation of China (No. 2013RMFH012), the Province Natural Science Foundation of Hubei (No. 2012FFA096), and supported by the Fundamental Research Funds for the Central Universities (No. 302-274231). We thank all the authors whose work was included in this study.
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Wang, M., Liu, X., Jiang, G. et al. Relationship between LSD1 expression and E-cadherin expression in prostate cancer. Int Urol Nephrol 47, 485–490 (2015). https://doi.org/10.1007/s11255-015-0915-2
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DOI: https://doi.org/10.1007/s11255-015-0915-2