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Effects of sensory neuron-specific receptor agonist on bladder function in a rat model of cystitis induced by cyclophosphamide

  • Urology - Original Paper
  • Published:
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Abstract

Purpose

To investigate the effects of activation of sensory neuron-specific receptors (SNSRs) on cyclophosphamide (CYP) bladder overactivity in rats.

Methods

Female Sprague–Dawley rats (235–258 g) were used. Rats were injected with either CYP (200 mg/kg, intraperitoneally) or saline (control). Continuous cystometrograms (0.04 ml/min) were recorded 48 h after CYP or saline injection under urethane anesthesia. After stable micturition cycles were established, a selective rat SNSR1 agonist, bovine adrenal medulla 8-22 (BAM8-22), was administered intravenously or intrathecally.

Results

Cyclophosphamide treatment-induced higher baseline pressure and shorter intercontraction intervals compared with the control group. Intravenous administration of BAM8-22 at 10, 30 and 100 μg/kg significantly increased intercontraction intervals in the CYP-treated group. Intrathecal administration of BAM8-22 at 0.03, 0.1 and 0.3 μg also significantly increased intercontraction intervals in the CYP-treated group. Intravenous or intrathecal administration of BAM8-22 did not change baseline pressure or maximum voiding pressure in the CYP-treated group.

Conclusions

These findings indicate that activation of SNSRs can suppress CYP-induced bladder overactivity, probably due to suppression of bladder afferent activity.

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Acknowledgments

This study was supported by grants from NIH (DK088836 and P01 DK093424) and DOD (W81XWH-12-1-0565).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Urology, Tottori University Faculty of Medicine, 36-1 Nishi, Yonago, 683-8504, Japan

    Masashi Honda, Bunya Kawamoto, Naoto Kobayashi, Katsuya Hikita, Kuniyasu Muraoka, Takehiro Sejima & Atsushi Takenaka

  2. Department of Urology, University of Pittsburgh School of Medicine, Suite 700 Kaufmann Medical Building, 3471 Fifth Avenue, Pittsburgh, PA, 15213, USA

    Naoki Yoshimura

  3. Department of Pharmacology, Kochi University School of Medicine, Okocho Kohasu, Nankoku, 7838505, Japan

    Motoaki Saito

  4. Department of Urology, William Beaumont Hospital, 3535 W 13 Mile Rd #404, Royal Oak, MI, 48073, USA

    Michael B. Chancellor

Authors
  1. Masashi Honda
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  2. Naoki Yoshimura
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  3. Bunya Kawamoto
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  4. Naoto Kobayashi
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  5. Katsuya Hikita
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  6. Kuniyasu Muraoka
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  7. Motoaki Saito
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  8. Takehiro Sejima
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  9. Michael B. Chancellor
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  10. Atsushi Takenaka
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Corresponding author

Correspondence to Naoki Yoshimura.

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Honda, M., Yoshimura, N., Kawamoto, B. et al. Effects of sensory neuron-specific receptor agonist on bladder function in a rat model of cystitis induced by cyclophosphamide. Int Urol Nephrol 46, 1953–1959 (2014). https://doi.org/10.1007/s11255-014-0734-x

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  • DOI: https://doi.org/10.1007/s11255-014-0734-x

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