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Differential effects of coenzyme Q10 and α-lipoic acid on two models of in vitro oxidative damage to the rabbit urinary bladder

  • Urology – Original Paper
  • Published:
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Abstract

Purpose

Partial bladder outlet obstruction (PBOO) in rabbits causes free radical production through ischemia and reperfusion within the bladder smooth muscle and mucosa. We had previously shown that pretreatment of rabbits with a combination of α-lipoic acid (αLA) and coenzyme Q10 (CoQ) protected the bladder from contractile and metabolic dysfunctions mediated by PBOO. In this study, we examined the ability of pretreatment with αLA and CoQ combination in rabbits to protect the bladder from contractile damage mediated by either hydrogen peroxide (H2O2) or in vitro ischemia–reperfusion (I/R) which represents two in vitro models of oxidative damage.

Methods and materials

Eight adult male New Zealand white rabbits were pretreated with CoQ and αLA orally for four weeks. Eight adult male control rabbits were given vehicle. Eight full-thickness bladder strips were isolated from each of 4 treated and 4 control rabbit bladders, and a dose–response curve to H2O2 (0.1–0.8%) was generated. Similarly, isolated strips of bladder from the remaining 4 control and 4 treated rabbits were subjected to 1 h of ischemia (no oxygen without glucose) followed by 2 h of incubation in oxygenated buffer with glucose. The effects on the contractile responses to field stimulation (FS) at 2, 8, and 32 Hz, carbachol, and potassium chloride (KCl) were determined.

Results

H2O2 reduced the contractile responses to KCl and carbachol to a significantly greater degree than to FS, whereas I/R reduced the contractile responses to FS to a significantly greater degree than to KCl and carbachol. Pretreatment of the rabbits with the combination of CoQ and αLA significantly protected the bladder from the damaging effects of I/R, but had virtually no effect on the damaging effects of H2O2.

Conclusion

Although both H2O2 and I/R are in vitro models of oxidative free radical damage to bladder smooth muscle, they have significantly different methods of action and different sensitivities to antioxidants.

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Acknowledgments

This material is based upon work supported in part by the Office of Research and Development Medical Research Service, Department of Veteran’s Affairs, and in part by the Capital Region Medical Research Foundation.

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Authors and Affiliations

  1. Albany College of Pharmacy and Health Sciences, 106 New Scotland Ave, Albany, NY, 12208, USA

    Hsin T. Li, Robert E. Leggett & Robert M. Levin

  2. Albany Medical College, Albany, NY, USA

    Robert M. Levin

  3. Stratton VA Medical Center, Albany, NY, USA

    Catherine Schuler, Robert E. Leggett & Robert M. Levin

Authors
  1. Hsin T. Li
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  2. Catherine Schuler
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  3. Robert E. Leggett
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  4. Robert M. Levin
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Correspondence to Robert M. Levin.

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Li, H.T., Schuler, C., Leggett, R.E. et al. Differential effects of coenzyme Q10 and α-lipoic acid on two models of in vitro oxidative damage to the rabbit urinary bladder. Int Urol Nephrol 43, 91–97 (2011). https://doi.org/10.1007/s11255-010-9771-2

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  • DOI: https://doi.org/10.1007/s11255-010-9771-2

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