Abstract
Objective
To understand the role of P-selectin (CD62P) and CD44 in mediating immune inflammation in the nephrotic process of children with IgA nephropathy (IgAN), cooperative expression of CD62P and CD44 in peripheral blood and renal tissues of IgAN children was investigated and its association with changes of histopathologic, serologic, and urinary properties was tested.
Material and methods
Forty-six IgAN children were divided into three groups according to pathologic grades and clinical features. Fifteen blood samples from normal children and four normal renal biopsy specimens were used as controls. Plasma level of CD62P was detected by double antibody sandwich immunoradiometric assay; ELISA was used to determine serum level of CD44. Expression of CD62P and CD44 in renal tissues was determined by immunohistochemistry.
Results
Cooperative expression of CD62P and CD44 was detected in renal tissues and peripheral blood of IgAN children. Altered expression of CD62P and CD44 in peripheral blood significantly correlated not only with hematuria, proteinuria, serum cholesterol, and albumin, and with urine NAG and β2-MG, but also with degree of tubulointerstitial injury in IgAN children.
Conclusion
The evidence supported CD62P and CD44 as initial and promoting factors mediating immune inflammation in the nephrotic process in IgAN children. The cooperative expression profiles of CD62P and CD44 in renal tissues and peripheral blood combined with serologic and urinary predictors may be important in diagnosis of progression in children with IgAN.
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Acknowledgements
The authors are indebted to Dr. Dai K for English proofreading. We are grateful to Dr. Liu XQ for valuable discussion and to Ms. He JH for her gentle and prompt cooperation regarding immuno-histochemical detection. This research was partly supported by funds from Guang Dong Provincial Science and Technology Project of China (Grant No. 2008B030301070), Guang Dong Provicial Medical Science Program of China (Grant No. B2008027).
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Qiaoling, Z., Xiaoyun, J., Wei, W. et al. Altered P-selectin and CD44 expression in the renal tissues and peripheral blood of children with IgA nephropathy. Int Urol Nephrol 41, 703–711 (2009). https://doi.org/10.1007/s11255-008-9512-y
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DOI: https://doi.org/10.1007/s11255-008-9512-y