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Progenitor cells are responsible for formation primary epithelial cultures in the prostate epithelial model

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Abstract

The adult stem cells (ASC) are supposed to regenerate epithelium. We hypothesized prostate epithelial CD133-positive ASC to be responsible for establishing the primary cell culture. The prostate epithelial stem cells were isolated using anti-CD133 microbeads in order to form different cell populations. The morphology of cultures developed from CD133+ and CD133− prostate epithelial cells were compared with prostate epithelium cell culture obtained after simple isolation procedure. Four 8-week-old Wistar rats were used in the experiment and six cultures were obtained. Double CD133+ and CD133− cultures from two rats were established after enzymatic digestion and positive selection by SuperMACS device, and two non-selected CD133+/CD133− cultures were developed by simple prostate epithelial cell isolation from two other rats. The epithelial nature was confirmed by anti-cytokeratine antibodies. It was observed that growth of the CD133+/CD133− and CD133+culture resembled epithelial-like prostate cell culture. It was not possible to establish epithelial-like culture from CD133− cell population. The primary epithelial cell culture collapsed in a few days after the CD133-positive ASC were removed. We concluded that the epithelial progenitor cells are responsible for establishing primary prostate epithelial cultures in vitro.

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References

  1. Diaz-Flores L Jr, Madrid JF, Gutierrez R, Varela H, Valladares F, Alvarez-Arguelles H, Diaz-Flores L (2006) Adult stem and transit-amplifying cell location. Histol Histopathol 21:995–1027

    PubMed  Google Scholar 

  2. Peehl DM (2005) Primary cell cultures as models of prostate cancer development. Endocrine-Related Cancer 12:19–47

    Article  PubMed  CAS  Google Scholar 

  3. Uzgare AR, Xu Y, Isaacs JT (2004) In vitro culturing and characteristics of transit amplifying epithelial cells from human prostate tissue. J Cell Biochem 91:196–205

    Article  PubMed  CAS  Google Scholar 

  4. Xin L, Ide H, Kim Y, Dubey P, Witte ON (2003) In vivo regeneration of murine prostate from dissociated cell populations of postnatal epithelia and urogenital sinus mesenchyme. Proc Natl Acad Sci USA 100:11896–11903

    Article  PubMed  CAS  Google Scholar 

  5. Azuma M, Hirao A, Takubo K, Hamaguchi I, Kitamura T, Suda T (2005) A quantitative matrigel assay for assessing repopulating capacity of prostate stem cells. Biochem Biophys Res Commun 338:1164–1170

    Article  PubMed  CAS  Google Scholar 

  6. Taylor RA, Cowin PA, Cunha GR, Pera M, Trounson AO, Pedersen J, Risbridger GP (2006) Formation of human prostate tissue from embryonic stem cells. Nat Methods 3:179–81

    Article  PubMed  CAS  Google Scholar 

  7. Schmelz M, Moll R, Hesse U, Prasad AR, Gandolfi JA, Hasan SR, Bartholdi M, Cress AE (2005) Identification of a stem cell candidate in the normal human prostate gland. Eur J Cell Biol 84:341–354

    Article  PubMed  CAS  Google Scholar 

  8. Heer R, Collins AT, Robson CN, Shenton BK, Leung HY (2006) KGF suppresses alpha2beta1 integrin function and promotes differentiation of the transient amplifying population in human prostatic epithelium. J Cell Sci 119:1416–1424

    Article  PubMed  CAS  Google Scholar 

  9. Richardson GD, Robson CN, Lang SH, Neal DE, Maitland NJ, Collins AT (2004) CD133, a novel marker for human prostatic epithelial stem cells. J Cell Sci 117:3539–3545

    Article  PubMed  CAS  Google Scholar 

  10. Rizzo S, Attard G, Hudson DL (2005) Prostate epithelial stem cells. Cell Prolif 38:363–374

    Article  PubMed  CAS  Google Scholar 

  11. Peehl DM, Stamey TA (1986) Growth responses of normal, benign hyperplastic and malignant human prostatic epithelial cells in vitro to cholera toxin, pituary extract and hydrocortisone. Prostate 8:51–61

    Article  PubMed  CAS  Google Scholar 

  12. Ravindranath N, Dym M (1999) Isolation of rat ventral prostate basal and luminal epithelial cells by the STAPUT technique. Prostate 41:173–180

    Article  PubMed  CAS  Google Scholar 

  13. Planz B, Tabatabaei S, Kirley SD et al (2004) Studies on the differentiation pathway and growth characteristics of epithelial culture cells of the human prostate. Prostate Cancer Prostatic Dis 7:73–83

    Article  PubMed  CAS  Google Scholar 

  14. Bhatt RI, Brown MD, Hart CA et al (2003) Novel method for the isolation and characterisation of the putative prostatic stem cell. Cytometry A54:89–99

    Article  Google Scholar 

  15. Goto K, Salm SN, Coetzee S, Xiong X, Burger PE, Shapiro E, Lepor H, Moscatelli D, Wilson EL (2006) Proximal prostate stem cells are programmed to regenerate a proximal-distal ductal axis. Stem Cells 24:1859–1868

    Article  PubMed  CAS  Google Scholar 

  16. Signoretti S, Loda M (2006) Defining cell lineages in the prostate epithelium. Cell Cycle 5:138–141

    PubMed  CAS  Google Scholar 

  17. Wang S, Garcia AJ, Wu M, Lawson DA, Witte ON, Wu H (2006) Pten deletion leads to the expansion of a prostatic stem/progenitor cell subpopulation and tumor initiation. Proc Natl Acad Sci USA 103:1480–1485

    Article  PubMed  CAS  Google Scholar 

  18. Signoretti S, Waltregny D, Dilks J, Isaac B, Lin D, Garraway L, Yang A, Montironi R, McKeon F, Loda M (2000) p63 is a prostate basal cell marker and is required for prostate development. Am J Pathol 157:1769–1775

    PubMed  CAS  Google Scholar 

  19. Wang XD, Leow CC, Zha J, Tang Z, Modrusan Z, Radtke F, Aguet M, de Sauvage FJ, Gao WQ (2006) Notch signaling is required for normal prostatic epithelial cell proliferation and differentiation. Dev Biol 290:66–80

    Article  PubMed  CAS  Google Scholar 

  20. Sawicki JA, Rothman CJ (2002) Evidence for stem cells in cultures of mouse prostate epithelial cells. Prostate 50:46–53

    Article  PubMed  CAS  Google Scholar 

  21. van Leenders G, Dijkman H, Hulsbergen-van de Kaa C, Ruiter D, Schalken J (2000) Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. Lab Invest 80:1251–1258

    PubMed  Google Scholar 

  22. Hudson DL, O’Hare M, Watt FM, Masters JR (2000) Proliferative heterogeneity in the human prostate: evidence for epithelial stem cells. Lab Invest 80:1243–1250

    Article  PubMed  CAS  Google Scholar 

  23. Lam JS, Reiter RE (2006) Stem cells in prostate and prostate cancer development. Urol Oncol 24:131–140

    PubMed  CAS  Google Scholar 

  24. Hayward SW, Wang Y, Cao M, Hom YK, Zhang B, Grossfeld GD, Sudilovsky D, Cunha GR (2001) Malignant transformation in a nontumorigenic human prostatic epithelial cell line. Cancer Res 61:8135–8142

    PubMed  CAS  Google Scholar 

  25. Signoretti S, Loda M. (2006) Prostate stem cells: From development to cancer. Semin Cancer Biol (in press)

  26. Bavik C, Coleman I, Dean JP, Knudsen B, Plymate S, Nelson PS (2006) The gene expression program of prostate fibroblast senescence modulates neoplastic epithelial cell proliferation through paracrine mechanisms. Cancer Res 66:794–802

    Article  PubMed  CAS  Google Scholar 

  27. Patrawala L, Calhoun T, Schneider-Broussard R, Li H, Bhatia B, Tang S, Reilly JG, Chandra D, Zhou J, Claypool K, Coghlan L, Tang DG (2006) Highly purified CD44+ prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells. Oncogene 25:1696–1708

    Article  PubMed  CAS  Google Scholar 

  28. Sharma N, Seftor RE, Seftor EA, Gruman LM, Heidger PM Jr, Cohen MB, Lubaroff DM, Hendrix MJ (2002) Prostatic tumor cell plasticity involves cooperative interactions of distinct phenotypic subpopulations: role in vasculogenic mimicry. Prostate 50:189–201

    Article  PubMed  Google Scholar 

  29. Koeneman KS (2006) Prostate cancer stem cells, telomerase biology, epigenetic modifiers, and molecular systemic therapy for the androgen-independent lethal phenotype. Urol Oncol 24:119–121

    PubMed  CAS  Google Scholar 

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Acknowledgement

Authors thank Prof. Donna Peehl (Stanford University School of Medicine, Stanford, USA) for help in the methodology of establishing primary epithelial prostate cultures.

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Authors and Affiliations

  1. Department of Tissue Engineering, Chair of Medical Biology, Nicolaus Copernicus University, Karlowicza 24, 85-092, Bydgoszcz, Poland

    Tomasz Drewa

  2. Department of Urology, Nicolaus Copernicus University, Bydgoszcz, Poland

    Tomasz Drewa

  3. Department of Pediatric Hematology and Oncology, Nicolaus Copernicus University, Bydgoszcz, Poland

    Jan Styczynski

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  1. Tomasz Drewa
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Correspondence to Tomasz Drewa.

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Drewa, T., Styczynski, J. Progenitor cells are responsible for formation primary epithelial cultures in the prostate epithelial model. Int Urol Nephrol 39, 851–857 (2007). https://doi.org/10.1007/s11255-006-9105-6

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  • DOI: https://doi.org/10.1007/s11255-006-9105-6

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