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Progenitor cells are responsible for formation primary epithelial cultures in the prostate epithelial model

International Urology and Nephrology volume 39, pages 851–857 (2007)Cite this article

Abstract

The adult stem cells (ASC) are supposed to regenerate epithelium. We hypothesized prostate epithelial CD133-positive ASC to be responsible for establishing the primary cell culture. The prostate epithelial stem cells were isolated using anti-CD133 microbeads in order to form different cell populations. The morphology of cultures developed from CD133+ and CD133− prostate epithelial cells were compared with prostate epithelium cell culture obtained after simple isolation procedure. Four 8-week-old Wistar rats were used in the experiment and six cultures were obtained. Double CD133+ and CD133− cultures from two rats were established after enzymatic digestion and positive selection by SuperMACS device, and two non-selected CD133+/CD133− cultures were developed by simple prostate epithelial cell isolation from two other rats. The epithelial nature was confirmed by anti-cytokeratine antibodies. It was observed that growth of the CD133+/CD133− and CD133+culture resembled epithelial-like prostate cell culture. It was not possible to establish epithelial-like culture from CD133− cell population. The primary epithelial cell culture collapsed in a few days after the CD133-positive ASC were removed. We concluded that the epithelial progenitor cells are responsible for establishing primary prostate epithelial cultures in vitro.

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Acknowledgement

Authors thank Prof. Donna Peehl (Stanford University School of Medicine, Stanford, USA) for help in the methodology of establishing primary epithelial prostate cultures.

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Affiliations

  1. Department of Tissue Engineering, Chair of Medical Biology, Nicolaus Copernicus University, Karlowicza 24, 85-092, Bydgoszcz, Poland

    Tomasz Drewa

  2. Department of Urology, Nicolaus Copernicus University, Bydgoszcz, Poland

    Tomasz Drewa

  3. Department of Pediatric Hematology and Oncology, Nicolaus Copernicus University, Bydgoszcz, Poland

    Jan Styczynski

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  1. Tomasz Drewa
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  2. Jan Styczynski
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Correspondence to Tomasz Drewa.

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Drewa, T., Styczynski, J. Progenitor cells are responsible for formation primary epithelial cultures in the prostate epithelial model. Int Urol Nephrol 39, 851–857 (2007). https://doi.org/10.1007/s11255-006-9105-6

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  • DOI: https://doi.org/10.1007/s11255-006-9105-6

Keywords

  • Stem cells
  • Primary epithelial cell culture
  • Cell culture establishing