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Renoprotective effects of tea catechin in streptozotocin- induced diabetic rats

  • Michiyo Hase
  • Tetsuya BabazonoEmail author
  • Sachiko Karibe
  • Naohide Kinae
  • Yasuhiko Iwamoto
Original Article

Abstract

Tea catechins, a class of flavonoids, are suggested to have biological effects, possibly mediated through their antioxidative properties. Recent data indicated that tea catechins suppressed proliferative changes in glomeruli and inhibited the development of glomerulosclerosis in partially nephrectomized rats. We thus sought to determine whether tea catechins may protect against renal dysfunction in streptozotocin-induced diabetic rats. Four groups of male Sprague-Dawley rats (n=11–15 per group), with and without streptozotocin-induced diabetes, were treated with and without catechins (5 mg/day) administered in the drinking water for 12 weeks. At the end of the treatment period, 24-hour urinary albumin excretion rate (AER), serum lipid peroxides as thiobarbituric acid reactive substrates (TBARS) and blood pressure were measured. Renal glomerular volume and interstitial fibrosis were assessed morphologically. Albuminuria developed progressively in untreated diabetic rats, resulting in a mean AER of 559±124 (mean±SE) versus 63±7 µg/day/100 g body weight in non-diabetic rats at 12 weeks (P<0.001). Catechin treatment significantly reduced AER to 287±56 µg/day/100 g body weight in diabetic rats (P=0.017 versus untreated diabetic rats). Increased interstitial fibrosis in the kidney, observed in untreated diabetic rats, was completely normalized with catechin treatment. Serum levels of TBARS and blood pressure were comparable among the four groups. In conclusion, administration of tea catechin retards the progression of functional and morphological changes in the kidney of streptozotocin-induced diabetic rats.

Keywords

Diabetic nephropathy Glomerular hypertrophy Interstitial fibrosis Albuminuria Tea catechins 

References

  1. 1.
    Nishikawa T, Edelstein D, Du XL, et al (2000) Normalizing mitochondrial superoxide production blocks three pathways of hyperglycemic damage. Nature 404:787–790PubMedCrossRefGoogle Scholar
  2. 2.
    Terao J (1999) Dietary flavonoids as antioxidants in vivo: conjugated metabolites of (-)-epicatechin and quercetin participate in antioxidative defense in blood plasma. J Med Invest 46:159–168PubMedGoogle Scholar
  3. 3.
    Schroeter H, Spencer JP, Rice-Evans C, et al (2001) Flavonoids protect neurons from oxidized low-density-lipoprotein-induced apoptosis involving c-Jun N-terminal kinase (JNK), c-Jun and caspase-3. Biochem J 358:547–557PubMedCrossRefGoogle Scholar
  4. 4.
    Hasslacher C, Burklin E, Kopischke HG (1981) Inhibition of increased synthesis of glomerular basement membrane collagen in diabetic rats in vivo. Ren Physiol 4:108–111PubMedCrossRefGoogle Scholar
  5. 5.
    Rhee SJ, Kim MJ, Kwag OG (2002) Effects of green tea catechin on prostaglandin synthesis of renal glomerular and renal dysfunction in streptozotocin-induced diabetic rats. Asia Pac J Clin Nutr 11:232–236PubMedCrossRefGoogle Scholar
  6. 6.
    Takabayashi F, Harada N, Hara Y (1995) The effects of green tea catechins (Polyphenon) on DL-ethionine-induced acute pancreatitis. Pancreas 11:127–131PubMedCrossRefGoogle Scholar
  7. 7.
    Kinae N, Yamashita M, Esaki S, et al (1990) Inhibitory effects of tea extracts on the formation of advanced glycosylation products. The maillard reaction in food processing, human nutrition and physiology. Birkhauser Verleg, Basel, pp 221–226Google Scholar
  8. 8.
    Wallin BR, Rosengren B, Shertzer HG (1993) Lipoprotein oxidation and measurement of thibarbituric acid reacting substances formation in a single microtiter plate: its use for evaluation of antioxidants. Anal Biochem 208:10–15PubMedCrossRefGoogle Scholar
  9. 9.
    Kasahara Y, Ashihara Y (1981) Colorimetry of angiotensin-I converting enzyme activity in serum. Clin Chem 27:1922–1925PubMedGoogle Scholar
  10. 10.
    Weibel ER (1979) Stereological methods. Practical methods for biological morphometry, vol 1. Academic Press, London, pp 1–415Google Scholar
  11. 11.
    Fujitsuka N, Kurogi A, Hattori T, et al (1997) Effects of Onpi-to (TJ-8117) and Epicatechin-3-O-gallate on the proliferating changes in glomeruli of 5/6 nephrectomized rats. Jpn J Nephrol 39:693–700Google Scholar
  12. 12.
    Yokozawa T, Chung HY, He LQ, et al (1996) Effectiveness of green tea tannin on rats with chronic renal failure. Biosci Biotech Biochem 60:1000–1005CrossRefGoogle Scholar
  13. 13.
    Yokozawa T, Oura H, Hattori M, et al (1993) Inhibitory effect of tannin in green tea on the proliferation of mesangial cells. Nephron 65:596–600PubMedCrossRefGoogle Scholar
  14. 14.
    Ichikawa I, Kiyama S, Yoshioka T (1993) Renal antioxidant enzymes: their regulation and function. Kidney Int 45:1–9Google Scholar
  15. 15.
    Christ M, Bauersachs J, Liebetrau C, et al (2002) Glucose increases endothelial-dependent superoxide formation in coronary arteries by NAD(P)H oxidase activation. Attenuation by the 3-Hydroxy-3-Methylglutaryl coenzyme A reductase inhibitor atorvastatin. Diabetes 51:2648–2652PubMedGoogle Scholar
  16. 16.
    Ceriello A, Morocutti A, Mercuri F, et al (2000) Defective intracellular antioxidant enzyme production in type 1 diabetic patients with nephropathy. Diabetes 49:2170–2177PubMedGoogle Scholar
  17. 17.
    Ha H, Kim KH (1995) Role of oxidative stress in the development of diabetic nephropathy. Kidney Int 48(Suppl. 51):S18–S21Google Scholar
  18. 18.
    Kedziora-Kornatowska K, Szram S, Kornatowski T, et al (2002) The effect of verapamil on the antioxidant defence system in diabetic kidney. Clin Chim Acta 322:105–112PubMedCrossRefGoogle Scholar
  19. 19.
    de Cavanagh EM, Inserra F, Toblli J, et al (2001) Enalapril attenuates oxidative stress in diabetic rats. Hypertension 38:1130–1136PubMedGoogle Scholar
  20. 20.
    Packer L, Rimbach G, Virgili F (1999) Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, pycnogenol. Free Radic Biol Med 27:704–724PubMedCrossRefGoogle Scholar
  21. 21.
    Brenner BM, Cooper ME, de Zeeuw D, et al (2001) Effects of losartan on renal and cardiovascular outcomes in patients with type 2 diabetes and nephropathy. New Engl J Med 345:861–869PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Michiyo Hase
    • 1
    • 2
  • Tetsuya Babazono
    • 1
    • 2
    Email author
  • Sachiko Karibe
    • 2
  • Naohide Kinae
    • 3
  • Yasuhiko Iwamoto
    • 2
  1. 1.Division of Nephrology and Hypertension, Diabetes CenterTokyo Women’s Medial University School of MedicineTokyoJapan
  2. 2.Department of Medicine, Diabetes CenterTokyo Women’s Medial University School of MedicineTokyoJapan
  3. 3.Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental SciencesUniversity of ShizuokaShizuokaJapan

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