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Digital subtraction angiography and computer assisted image analysis for the evaluation of the antiangiogenetic effect of ionizing radiation on tumor angiogenesis

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Abstract

Purpose:

The aim of the present study was to evaluate and quantify the antiangiogenetic effect of ionizing radiation on tumor angiogenesis using digital subtraction angiography (DSA) in conjunction with computer assisted image analysis (CAIA).

Methods:

Walker 256 carcinosarcoma was inoculated in both glutei of 12 Wistar rats. When the tumors reached a diameter of 1.5 cm, local irradiation of the right gluteus was performed. The left gluteus of each animal served as a control. After 24 hours of irradiation, angiography was performed, and images where digitized and subsequently processed. The effect of irradiation was observed both in big and small vessels (smaller or greater than 200 μm).

Results:

Irradiated areas of both small and big vessels showed a statistically significant reduction in both total vessel area and length. Small vessels showed a greater trend toward suppression by irradiation (not statistically significant).

Conclusion:

Irradiation had a deleterious effect in both macro- and micro-blood supply of a tumor. The use of CAIA enhanced the efficacy of DSA and enabled the in vivo identification of the effect of irradiation on various caliber vessels as well as the ratios of total length and total area of small and big vessels.

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Authors and Affiliations

  1. Department of Urology, School of Medicine, University of Patras Medical School, University Hospital, Rio, Patras, 26-500, Greece

    Evangelos N. Liatsikos, Anastasios Athanassopoulos, Petros Perimenis & George A. Barbalias

  2. Department of Radiology, School of Medicine, University of Patras, Patras, Greece

    Dimitrios Siablis, Dimitrios Karnabatidis & Dimitrios Kardamakis

  3. Department of Medical Physics, School of Medicine, University of Patras, Patras, Greece

    George C. Kagadis, George C. Sakelaropoulos & George Nikiforidis

  4. Department of Surgery, University of Patras, School of Medicine, Patras, Greece

    John Maroulis

Authors
  1. Dimitrios Siablis
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  2. Evangelos N. Liatsikos
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  3. Dimitrios Karnabatidis
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  4. George C. Kagadis
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  5. George C. Sakelaropoulos
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  6. John Maroulis
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  7. Dimitrios Kardamakis
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  8. Anastasios Athanassopoulos
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  9. Petros Perimenis
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  10. George Nikiforidis
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  11. George A. Barbalias
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Corresponding author

Correspondence to George A. Barbalias.

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Siablis, D., Liatsikos, E.N., Karnabatidis, D. et al. Digital subtraction angiography and computer assisted image analysis for the evaluation of the antiangiogenetic effect of ionizing radiation on tumor angiogenesis. Int Urol Nephrol 38, 407–411 (2006). https://doi.org/10.1007/s11255-005-3617-3

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  • DOI: https://doi.org/10.1007/s11255-005-3617-3

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