Abstract
Urban wildlife faces a novel set of challenges resulting in selective pressure that can lead to population-level changes. We studied Australian water dragons (Intellagama lesueurii) from urban and natural populations to test if urban populations differed in body size, shape, and performance capacity. If urban-derived morphology has arisen through selection, we predicted distinct morphological differences between wild dragons from urban and natural areas in both adult and hatchling life-stages. Urban hatchlings were morphologically distinct (shorter body lengths and longer limbs) from natural populations, while urban adult males continued this trend but only for body size (shorter body lengths). We then experimentally reared hatchlings originating from urban and natural populations within urban- and natural-style enclosures (2 × 2 factorial design) for a year to determine if differences in morphology and performance capacity (sprint speed, endurance, and clinging ability) were related to either the individual’s origin population or developmental environment. Yearlings reared in urban-style enclosures, irrespective of population origin, had smaller body sizes compared to those from natural-style enclosures, suggesting developmental environment was affecting their morphology. Despite this difference in body size, yearling dragon performance capacity was not significantly different between treatments. Overall, this study provides evidence of a complex relationship driving urban-divergent morphology – whereby urban dragons emerge as smaller hatchlings with longer limbs (innate traits) and are then further influenced by the urban environments that they develop in (phenotypic plasticity); however, and potentially owing to behavioral, ecological, and demographical differences, these changes appear to be sex-specific.
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Data Availability
Data and R code are available from the Open Science Framework at https://osf.io/gk6jh/.
Change history
21 December 2020
Springer Nature’s version of this paper was updated to present the correct information in the Code availability section.
References
Alberti M, Correa C, Marzluff JM, Hendry AP, Palkovacs EP, Gotanda KM, Hunt VM, Apgar TM, Zhou Y (2017) Global urban signatures of phenotypic change in animal and plant populations. PNAS 114:8951–8956. https://doi.org/10.1073/pnas.1606034114
Allen V, Elsey RM, Jones N, Wright J, Hutchinson JR (2010) Functional specialization and ontogenetic scaling of limb anatomy in Alligator mississippiensis. J Anat 216:423–445. https://doi.org/10.1111/j.1469-7580.2009.01202.x
Ammanna VH, Saidapur SK, Shanbhag BA (2018) Perch associated expression of phenotypic plasticity in limb development and sprint speed in Agamid lizard, Calotes versicolor: A laboratory study. Asian Herpetol Res 9:175–181. https://doi.org/10.16373/j.cnki.ahr.180005
Angilletta MJ Jr, Wilson RS, Niehaus AC, Sears MW, Navas CA, Ribeiro PL (2007) Urban physiology: city ants possess high heat tolerance. PLoS One 2:e258. https://doi.org/10.1371/journal.pone.0000258
Arnfield AJ (2003) Two decades of urban climate research: a review of turbulence, exchanges of energy and water, and the urban heat island. Int J Climatol 23:1–26. https://doi.org/10.1002/joc.859
Arnold SJ (1983) Morphology, performance and fitness. Am Zool 23:347–361. https://doi.org/10.1093/icb/23.2.347
Arthur KE, Boyle MC, Limpus CJ (2008) Ontogenetic changes in diet and habitat use in green sea turtle (Chelonia mydas) life history. Mar Ecol Prog Ser 362:303–311. https://doi.org/10.3354/meps07440
Atwell JW, Cardoso C, Whittaker DJ, Campbell-Nelson S, Robertson KW, Ketterson ED (2012) Boldness behavior and stress physiology in a novel urban environment suggest rapid correlated evolutionary adaptation. Behav Ecol 5:960–969. https://doi.org/10.1093/beheco/ars059
Baird TA, Baird TD, Shine R (2012) Aggressive transition between alternative male social tactics in a long-lived Australian dragon (Physignathus lesueurii) living at high density. PLoS One 7:e41819. https://doi.org/10.1371/journal.pone.0041819
Baird TA, Lovern MB, Shine R (2014) Heightened aggression and winning contests increase corticosterone but decrease testosterone in male Australian water dragons. Horm Behav 66:393–400. https://doi.org/10.1016/j.yhbeh.2014.05.008
Barnett CA. 2015. Urban hymns how urban environments affect bird populations and avian singing behavior. In: Mahala G (ed) Seabirds and songbirds: habitat preferences, conservation and migratory behavior. Nova Science Publisher, Inc. Hauppauge, USA, pp 115–134
Bates D, Mächler M, Bolker B, Walker S (2015) Fitting linear mixed-effects models using lme4. J Stat Softw 67:1–48. https://doi.org/10.18637/jss.v067.i01
Baxter-Gilbert J, Mühlenhaupt M, Whiting MJ (2018a) Comparability and repeatability of three commonly used methods for measuring endurance capacity. J Exp Zool 327:583–591. https://doi.org/10.1002/jez.2145
Baxter-Gilbert J, Riley JL, Whiting MJ (2018b) Runners and fighters: clutch effects and body size drive innate antipredator behaviour in hatchling lizards. Behav Ecol Sociobiol 72:97. https://doi.org/10.1007/s00265-018-2505-7
Baxter-Gilbert J, Riley JL, Whiting MJ (2019) Bold new world: urbanization promotes an innate behavioral trait in a lizard. Behav Ecol Sociobiol 73:105. https://doi.org/10.1007/s00265-019-2713-9
Baxter-Gilbert JH, Whiting MJ (2018) Street fighters: bite force, injury rates, and density of urban Australian water dragons (Intellagama lesueurii). Austral Ecol 44:255–264. https://doi.org/10.1111/aec.12670
Bonier F (2012) Hormones in the city: endocrine ecology of urban birds. Horm Behav 61:763–772. https://doi.org/10.1016/j.yhbeh.2012.03.016
Bouchard SS, Bjorndal KA (2005) Ontogenetic Diet Shifts and Digestive Constraints in the Omnivorous Freshwater Turtle Trachemys scripta. Physiol Biochem Zool 79:150–158 https://www.jstor.org/stable/10.1086/498190
Brown CR, Bomberger Brown M (2013) Where has all the road kill gone? Current Biol 23:R233–R234. https://doi.org/10.1016/j.cub.2013.02.023
Brown RM, Gist DH, Taylor DH (1995) Home range ecology of an introduced population of the European wall lizard Podarcis muralis (Lacertilia; Lacertidae) in Cincinnati, Ohio. Am Midl Nat 133:344–359. https://doi.org/10.2307/2426399
Campbell-Staton SC, Winchell KM, Rochette NC, Fredette J, Maayan I, Schweizer RM, Catchen J (2020) Parallel selection on thermal physiology facilitates repeated adaptation of city lizards to urban heat islands. Nat Ecol Evol 4:652–658. https://doi.org/10.1038/s41559-020-1131-8
Careau V, Wilson RS (2017) Of uberfleas and krakens: detecting trade-offs using mixed models. Integr Comp Biol 57:362–371. https://doi.org/10.1093/icb/icx015
Chevin LM, Lande R (2011) Adaptation to marginal habitats by evolution of increased phenotypic plasticity. J Evol Biol 24:1462–1476. https://doi.org/10.1111/j.1420-9101.2011.02279.x
Cogger HG (2014) Reptiles and amphibians of Australia, 7th edn. Reed Books, Chatswood
Collette BB (1961) Correlations between ecology and morphology in anoline lizards from Havana, Cuba, and southern. Bull Mus Comp Zool 125:135–162
Dale AG, Frank SD (2014) Urban warming trumps natural enemy regulation of herbivorous pests. Ecol Appl 24:1596–1607. https://doi.org/10.1890/13-1961.1
de Solla SR, Bishop CA, Brooks RJ (2002) Sexually dimorphic morphology of hatchling snapping turtles (Chelydra serpentina) from contaminated and reference sites in the Great Lakes and St. Lawrence River basin, North America. Environ Toxicol Chem 21:922–929. https://doi.org/10.1002/etc.5620210506
Delaney KS, Riley SP, Fisher RN (2010) A rapid, strong, and convergent genetic response to urban habitat fragmentation in four divergent and widespread vertebrates. PloS One 5:e12767. https://doi.org/10.1371/journal.pone.0012767
Diamond SE, Chick L, Perez A, Strickler SA, Martin RA (2017) Rapid evolution of ant thermal tolerance across an urban-rural temperature cline. Biol J Linn Soc 121:248–257. https://doi.org/10.1093/biolinnean/blw047
Dill AK, Sanger TJ, Battles AC, Johnson MA (2013) Sexual dimorphisms in habitat-specific morphology and behavior in the green anole lizard. J Zool 290:135–142. https://doi.org/10.1111/jzo.12020
Donihue CM, Herrel A, Fabre AC, Kamath A, Geneva AJ, Schoener TW, Kolbe JJ, Losos JB (2018) Hurricane-induced selection on the morphology of an island lizard. Nature 560:88–91. https://doi.org/10.1038/s41586-018-0352-3
Doody JS, Guarino E, Georges A, Corey B, Murray G, Ewert M (2006) Nest site choice compensates for climate effects on sex ratios in a lizard with environmental sex determination. Evol Ecol 20:307–330. https://doi.org/10.1007/s10682-006-0003-2
Doody JS, Harlow P, Douglass D, Thiem JD, Broadhurst B, Trembath D, Olsen J, Fuentes E, Rose T (2014) Patterns of predation and antipredator behavior in the Australian water dragon, Physignathus lesueurii. Herpetol Conserv Biol 9:48–56
Evans KL, Gaston KJ, Sharp SP, McGowan A, Hatchwell BJ (2009) The effect of urbanisation on avian morphology and latitudinal gradients in body size. Oikos 118:251–259. https://doi.org/10.1111/j.1600-0706.2008.17092.x
French SS, Fokidis HB, Moore MC (2008) Variation in stress and innate immunity in the tree lizard (Urosaurus ornatus) across an urban–rural gradient. J Comp Physiol B 178:997–1005. https://doi.org/10.1007/s00360-008-0290-8
French SS, Webb AC, Hudson SB, Virgin EE (2018) Town and country reptiles: A review of reptilian responses to urbanization. Integr Comp Biol 58:948–966. https://doi.org/10.1093/icb/icy052
Gardiner RZ, Doran E, Strickland K, Carpenter-Bundhoo L, Frère C (2014) A face in the crowd: a non-invasive and cost effective photo-identification methodology to understand the fine scale movement of eastern water dragons. PloS One 9:e96992. https://doi.org/10.1371/journal.pone.0096992
Garland T, Bennett AF, Daniels CB (1990) Heritability of locomotor performance and its correlates in a natural population. Experientia 46:530–533. https://doi.org/10.1007/BF01954257
Garland T, Else PL (1987) Seasonal, sexual, and individual variation in endurance and activity metabolism in lizards. Am J Physiol 252:R439–R449. https://doi.org/10.1152/ajpregu.1987.252.3.R439
Garland T Jr, Losos JB (1994) Ecological morphology of locomotor performance in squamate reptiles. In: Wainwright PC, Reilly SM (eds) Ecological morphology: integrative organismal biology. University of Chicago Press, Chicago, pp 240–302
Griffiths K (2006) Frogs and reptiles of the Sydney Region. Reed New Holland. Sydney
Gunderson AR, Siegel J, Leal M (2011) Tests of the contribution of acclimation to geographic variation in water loss rates of the West Indian lizard Anolis cristatellus. J Comp Physiol B 181:965–972. https://doi.org/10.1007/s00360-011-0576-0
Harlow PS, Harlow MF (1997) Captive reproduction and longevity in the eastern water dragon (Physignathus lesueurii). Herpetofauna 27:14–19
Harris SE, Munshi-South J, Obergfell C, O’Neill R (2013) Signatures of rapid evolution in urban and rural transcriptomes of white-footed mice (Peromyscus leucopus) in the New York metropolitan area. PLoS One 8:e74938. https://doi.org/10.1371/journal.pone.0074938
Horváth G, Rodríguez-Ruiz G, Martín J, López P, Herczeg G (2019) Maternal diet affects juvenile Carpetan rock lizard performance and personality. Ecol Evol 9:14476–14488. https://doi.org/10.1002/ece3.5882
Hosking C (2010) Husbandry guidelines for Australian water dragon, Physignathus lesueurii (Reptilia: Agamidae). Sydney, NSW, AU: Australian Museum. Available online: https://media.australianmuseum.net.au/media/dd/Uploads/Documents/25515/Water%20Dragon%20Husbandry%20Guidelines%20Chris%20Hosking.387ca78.pdf
Huey RB, Bennett AF, John-Alder H, Nagy KA (1984) Locomotor capacity and foraging behaviour of Kalahari lacertid lizards. Anim Behav 32:41–50. https://doi.org/10.1016/S0003-3472(84)80322-X
Hutton P, McGraw K (2016) Urban–rural differences in eye, bill, and skull allometry in house finches (Haemorhous mexicanus). Integr Comp Biol 56:1215–1224. https://doi.org/10.1093/icb/icw077
Iglesias S, Tracy C, Bedford G, Christian K (2012) Habitat differences in body size and shape of the Australian agamid lizard, Lophognathus temporalis. J Herpetol 46:297–303. https://doi.org/10.1670/11-084
Irschick DJ, Carlisle E, Elstrott J, Ramos M, Buckley C, Vanhooydonck B, Meyers J, Herrel A (2005) A comparison of habitat use, morphology, clinging performance and escape behaviour among two divergent green anole lizard (Anolis carolinensis) populations. Biol J Linn Soc 85:223–234. https://doi.org/10.1111/j.1095-8312.2005.00487.x
Irschick DJ, Macrini TE, Koruba S, Forman J (2000) Ontogenetic differences in morphology, habitat use, behavior, and sprinting capacity in two West Indian Anolis lizards. J Herpetol 34:444–451. https://doi.org/10.2307/1565368
Irschick DJ, Meyers JJ, Husak JF, Le Galliard JF (2008) How does selection operate on whole-organism functional performance capacities? A review and synthesis. Evol Ecol Res 10:177–196 http://www.evolutionary-ecology.com/abstracts/v10/2270.html
Johnson MT, Munshi-South J (2017) Evolution of life in urban environments. Science 358:eaam8327. https://doi.org/10.1126/science.aam8327
Kohlsdorf T, Garland T Jr, Navas CA (2001) Limb and tail lengths in relation to substrate usage in Tropidurus lizards. J Morphol 248:151–164. https://doi.org/10.1002/jmor.1026
Koenig J, Shine R, Shea G (2002) The dangers of life in the city: patterns of activity, injury and mortality in suburban lizards (Tiliqua scincoides). J Herpetol 36:62–68. https://doi.org/10.2307/1565803
Kolbe JJ, Losos JB (2005) Hind-limb length plasticity in Anolis carolinensis. J Herpetol 39:674–678. https://doi.org/10.1670/87-05N.1
Kralj-Fišer S, Schneider JM (2012) Individual behavioural consistency and plasticity in an urban spider. Anim Behav 84:197–204. https://doi.org/10.1016/j.anbehav.2012.04.032
Lande R (2009) Adaptation to an extraordinary environment by evolution of phenotypic plasticity and genetic assimilation. J Evol Biol 22:1435–1446. https://doi.org/10.1111/j.1420-9101.2009.01754.x
Lailvaux SP, Husak JF (2014) The life history of whole-organism performance. Q Rev Biol 89:285–318. https://doi.org/10.1086/678567
Le Galliard JF, Clobert J, Ferrière R (2004) Physical performance and Darwinian fitness in lizards. Nature 432:502. https://doi.org/10.1038/nature03057
Lleonart J, Salat J, Torres GJ (2000) Removing allometric effects of body size in morphological analysis. J Theor Biol 205:85–93. https://doi.org/10.1006/jtbi.2000.2043
Lenth RV (2016) Least-squares means: the R package lsmeans. J Stat Sofw 69:1–33. https://doi.org/10.18637/jss.v069.i01
Littleford-Colquhoun BL, Clemente C, Thompson G, Cristescu RH, Peterson N, Strickland K, Stuart-Fox D, Frère CH (2019b) How sexual and natural selection shape sexual size dimorphism: evidence from multiple evolutionary scales. Funct Ecol 33:1446–1458. https://doi.org/10.1111/1365-2435.13337
Littleford-Colquhoun BL, Clemente C, Whiting MJ, Ortiz-Barrientos D, Frére CH (2017) Archipelagos of the Anthropocene: rapid and extensive differentiation of native terrestrial vertebrates in a single metropolis. Mol Ecol 26:2466–2481. https://doi.org/10.1111/mec.14042
Littleford-Colquhoun BL, Weyrich LS, Kent N, Frere CH (2019a) City life alters the gut microbiome and stable isotope profiling of the eastern water dragon (Intellagama lesueurii). Mol Ecol 28:4592–4607. https://doi.org/10.1111/mec.15240
Lorioux S, DeNardo DF, Gorelick R, Lourdais O (2012) Maternal influences on early development: preferred temperature prior to oviposition hastens embryogenesis and enhances offspring traits in the Children’s python, Antaresia childreni. J Exp Biol 215:1346–1353. https://doi.org/10.1242/jeb.059113
Losos JB (1990) Ecomorphology, performance capability, and scaling of west Indian Anolis lizards: an evolutionary analysis. Ecol Monogr 60:369–388. https://doi.org/10.2307/1943062
Losos JB, Creer DA, Glossip D, Goellner R, Hampton A, Roberts G, Haskell N, Taylor P, Ettling J (2000) Evolutionary implications of phenotypic plasticity in the hindlimb of the lizard Anolis sagrei. Evolution 54:301–305. https://doi.org/10.1111/j.0014-3820.2000.tb00032.x
Losos JB, Sinervo B (1989) The effects of morphology and perch diameter on sprint performance of Anolis lizards. J Exp Biol 145:23–30
Lowe EC, Wilder SM, Hochuli DF (2014) Urbanisation at multiple scales is associated with larger size and higher fecundity of an orb-weaving spider. PLoS One 9:e105480. https://doi.org/10.1371/journal.pone.0105480
Marnocha E, Pollinger J, Smith TB (2011) Human-induced morphological shifts in an island lizard. Evol Appl 4:388–396. https://doi.org/10.1111/j.1752-4571.2010.00170.x
Mushinsky HR, Hebrard JJ, Vodopich DS (1982) Ontogeny of Water Snake Foraging Ecology. Ecology 63:1624–1629. https://doi.org/10.2307/1940102
Noble DW, Fanson KV, Whiting MJ (2014) Sex, androgens, and whole-organism performance in an Australian lizard. Biol J Linn Soc 111:834–849. https://doi.org/10.1111/bij.12252
Ouyang JQ, Isaksson C, Schmidt C, Hutton P, Bonier F, Dominoni D (2018) A new framework for urban ecology: an integration of proximate and ultimate responses to anthropogenic change. Integr Comp Biol 58:915–928. https://doi.org/10.1093/icb/icy110
Piza-Roca C, Kent N, Frère CH, Strickland K (2019) Presence of kin-biased social associations in a lizard with no parental care: the eastern water dragon (Intellagama lesueurii). Behav Ecol 30:1406–1415. https://doi.org/10.1093/beheco/arz093
Piza-Roca C, Strickland K, Schoeman D, Frère CH (2018) Eastern water dragons modify their social tactics with respect to the location within their home range. Anim Behav 144:27–36. https://doi.org/10.1016/j.anbehav.2018.08.001
Price TD, Qvarnström A, Irwin DE (2003) The role of phenotypic plasticity in driving genetic evolution. Proc Roy Soc B 270:1433–1440. https://doi.org/10.1098/rspb.2003.2372
Purwandana D, Ariefiandy A, Imansyah MJ, Seno A, Ciofi C, Letnic M, Jessop TS (2016) Ecological allometries and niche use dynamics across Komodo dragon ontogeny. Sci Nat 103:27. https://doi.org/10.1007/s00114-016-1351-6
Putman BJ, Gasca M, Blumstein DT, Pauly GB (2019) Downsizing for downtown: limb lengths, toe lengths, and scale counts decrease with urbanization in western fence lizards (Sceloporus occidentalis). Urban Ecosyst:1–11. https://doi.org/10.1007/s11252-019-00889-z
R Core Team. 2016. R: A Language and Environment for Statistical Computing; Available at: https://www.r-project.org
Shine R, Downes S (1999) Can pregnant lizards adjust their offspring phenotypes to environmental conditions? Oecologia 119:1–8. https://doi.org/10.1007/s004420050754
Sinervo B, Adolph SC (1994) Growth plasticity and thermal opportunity in Sceloporus lizards. Ecology 75:776–790. https://doi.org/10.2307/1941734
Slábová M, Frynta D (2007) Morphometric variation in nearly unstudied populations of the most studied mammal: the non-commensal house mouse (Mus musculus domesticus) in the Near East and Northern Africa. Zool Anz 246:91–101. https://doi.org/10.1016/j.jcz.2007.02.003
Sorci G, Clobert J (1997) Environmental maternal effects on locomotor performance in the common lizard (Lacerta vivipara). Evol Ecol 11:531–541. https://doi.org/10.1007/s10682-997-1509-y
Sorci G, Clobert J, Belichon S (1996) Phenotypic plasticity of growth and survival in the common lizard Lacerta vivipara. J Anim Ecology 65:781–790
Strickland K, Gardiner R, Schultz AJ, Frère CH (2014) The social life of eastern water dragons: sex differences, spatial overlap and genetic relatedness. Anim Behav 97:53–61. https://doi.org/10.1016/j.anbehav.2014.08.009
Stuart YE, Campbell TS, Hohenlohe PA, Reynolds RG, Revell LJ, Losos JB (2014) Rapid evolution of a native species following invasion by a congener. Science 346:463–466. https://doi.org/10.1126/science.1257008
Thompson MB (1993) Estimate of the population structure of the eastern water dragon, Physignathus lesueurii (Reptilia: Agamidae), along riverside habitat. Wildlife Res 20:613–619. https://doi.org/10.1071/WR9930613
Vanhooydonck B, James RS, Tallis J, Aerts P, Tadic Z, Tolley KA, Measey GJ, Herrel A (2014) Is the whole more than the sum of its parts? Evolutionary trade-offs between burst and sustained locomotion in lacertid lizards. Proc R Soc B 281:20132677. https://doi.org/10.1098/rspb.2013.2677
Vanhooydonck B, Van Damme R, Aerts P (2001) Speed and stamina trade-off in lacertid lizards. Evolution 55:1040–1048. https://doi.org/10.1111/j.0014-3820.2001.tb00620.x
Wang Y, Li SR, Zeng ZG, Liang L, Du WG (2017) Maternal food availability affects offspring performance and survival in a viviparous lizard. Funct Ecol 31:950–1956. https://doi.org/10.1111/1365-2435.12903
Warner DA, Lovern MB (2014) The maternal environment affects offspring viability via an indirect effect of yolk investment on offspring size. Physiol Biochem Zool 87:276–287. https://doi.org/10.1086/674454
Wild KH, Gienger CM (2018) Fire-disturbed landscapes induce phenotypic plasticity in lizard locomotor performance. J Zool 305:96–105. https://doi.org/10.1111/jzo.12545
Winchell KM, Maayan I, Fredette J, Revell L (2018) Linking locomotor performance to morphological shifts in urban lizards. Proc R Soc Biol Sci 285:20180229. https://doi.org/10.1098/rspb.2018.0229
Winchell KM, Reynolds RG, Prado-Irwin SR, Puente-Rolón AR, Revell LJ (2016) Phenotypic shifts in urban areas in the tropical lizard Anolis cristatellus. Evolution 70:1009–1022. https://doi.org/10.1111/evo.12925
Zuur AF, Leno EN, Elphick CS (2010) A protocol for data exploration to avoid common statistical problems. Methods Ecol Evol 1:3–14. https://doi.org/10.1111/j.2041-210X.2009.00001.x
Acknowledgments
We would like to thank the many individuals who assisted in fieldwork: P. Bolton, T. Damasio, C. Fryns, G. Hughes, F. Kar, S. Klopper, L. Monk-Whipp, M. Mühlenhaupt, D. Noble, and C. Wilson, as well as Taronga Zoo and the Lizard Lab animal care volunteers. We would also like to thank a host of anonymous reviewers for their comments that have improved this manuscript.
Funding
This research and JBG was supported by scholarship from Macquarie University and the Natural Sciences and Engineering Research Council (NSERC) during data collection, and a DSI-NRF Centre of Excellence for Invasion Biology postdoctoral fellowship. During this research JLR was supported by an Endeavour, Claude Leon Foundation, and a NSERC postdoctoral fellowship.
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JBG, CHF, and MJW conceived the idea for this study, JBG led the data collection, JLR led the statistical analysis, and JBG, JLR, CHF, and MJW contributed to the writing of the manuscript.
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Experimental protocols were approved by the Macquarie University Animal Ethics Committee (ARA # 2015/023), the Taronga Zoo Animal Ethics Committee (ARA # 3b/08/15), and New South Wales National Parks and Wildlife Services (scientific license # SL100570).
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Baxter-Gilbert, J., Riley, J.L., Frère, C.H. et al. Shrinking into the big city: influence of genetic and environmental factors on urban dragon lizard morphology and performance capacity. Urban Ecosyst 24, 661–674 (2021). https://doi.org/10.1007/s11252-020-01065-4
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DOI: https://doi.org/10.1007/s11252-020-01065-4