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Effects of different extenders, storage temperatures, and antioxidant supplementation on chilled semen quality: a review

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Abstract

The successful preservation of ram semen is essential to promote genetic variability, ensure semen transportation, and inseminate multiple ewes. Currently, either animal or plant-based lipoprotein-based extenders are used for semen preservation. Animal product-based extenders include milk and egg yolk, while soybean lecithin is a plant-based extender. Although extenders containing products of animal origin better preserve the quality of chilled semen, the in vivo efficacy after 24 h of storage is still of great concern. Storage temperature is another important and effective factor in preserving sperm quality, whereby different storage temperatures are adopted to enhance the storage life of sperm. Low temperatures (4–5 °C) better preserve sperm quality for a longer duration than high temperatures (15, 20, and 25 °C). Moreover, antioxidant supplementation has a positive impact on sperm quality during liquid storage. The current review summarizes the outcomes of various extenders, different storage temperatures, and antioxidant supplementation on the liquid storage of ram sperm.

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References

  • Abed Bresm, M.H., and Hassan Habeeb, H.M., 2023. Effect of Vitamin D3 on some Antioxidant Parameters in Chilled Semen in Awassi Ram, Institut Razi. Archives, 78, 721–727

    Google Scholar 

  • Acharya, M., Burke, J., Hansen, C., and Rorie, R.W., 2017. 21 Evaluation of semen extenders for short-term storage of ram semen at 4 C, Reproduction, Fertility and Development, 29, 118–118

    Article  Google Scholar 

  • Aitken, R.J., and Nixon, B., 2013. Sperm capacitation: a distant landscape glimpsed but unexplored, Molecular human reproduction, 19, 785–793

    Article  CAS  PubMed  Google Scholar 

  • Akalin, P.P., Bucak, M.N., Gungor, Ş., Baspinar, N., Coyan, K., Dursun, Ş., Pınar, İ., Aksoy, A., Karasor, Ö.F., and Bilgili, A., 2016. Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5 C, Small ruminant research, 137, 117–123

    Article  Google Scholar 

  • Akporhuaho, P., Isidahomen, C., and Otukpu, E., 2017. Effect of storage temperature on the motility of west African dwarf ram semen, Journal of Agriculture and Food Sciences, 15, 13–20

    Article  Google Scholar 

  • Allai, L., Druart, X., Contell, J., Louanjli, N., Moula, A.B., Badi, A., Essamadi, A., Nasser, B., and El Amiri, B., 2015. Effect of argan oil on liquid storage of ram semen in Tris or skim milk based extenders, Animal reproduction science, 160, 57–67

    Article  CAS  PubMed  Google Scholar 

  • Allai, L., Druart, X., Öztürk, M., BenMoula, A., Nasser, B., and El Amiri, B., 2016. Protective effects of Opuntia ficus-indica extract on ram sperm quality, lipid peroxidation and DNA fragmentation during liquid storage, Animal reproduction science, 175, 1–9

    Article  CAS  PubMed  Google Scholar 

  • Allai, L., Druart, X., Louanjli, N., Contell, J., Nasser, B., and El Amiri, B., 2017. Improvements of ram semen quality using cactus seed oil during liquid preservation in Tris egg yolk and skim milk based extenders, Small ruminant research, 151, 16–21

    Article  Google Scholar 

  • Anel, L., Alvarez, M., Martinez-Pastor, F., Garcia‐Macias, V., Anel, E., and De Paz, P., 2006. Improvement strategies in ovine artificial insemination, Reproduction in domestic animals, 41, 30–42

    Article  PubMed  Google Scholar 

  • Arando, A., Delgado, J.V., León, J.M., Nogales, S., Navas-González, F.J., Pizarro, M.G., and Pérez-Marín, C.C., 2019. Effect of three commercial extenders on sperm motility and fertility in liquid ram semen stored at 15 C or 5 C, Acta Veterinaria Hungarica, 67, 430–444

    Article  CAS  PubMed  Google Scholar 

  • Ashrafi, I., Kohram, H., Naijan, H., Bahreini, M., and Mirzakhani, H., 2012. Retraction: Effect of controlled and uncontrolled cooling rate on motility parameters of cryopreserved ram spermatozoa, BMC Research Notes, 5, 319

    Article  PubMed  PubMed Central  Google Scholar 

  • Azizunnesa, A., Zohara, B.F., Bari, F.Y., and Alam, M.G.S., 2013. Effects of concentrate supplementation on reproductive performances and semen quality of indigenous rams in Bangladesh, Journal of Embryo Transfer, 28, 325–335

    Article  Google Scholar 

  • Badi, A., Benmoula, A., El Khalil, K., Nasser, B., and El Amiri, B., 2018. Effect of age on Boujaâd ram semen quality extended in skim milk and tris egg yolk at 5° C, Revue Marocaine des Sciences Agronomiques et Vétérinaires, 6, 220–225

  • Bajia, N.P., Burdak, S., Yadav, S.P., and Kumar, A., 2022. Effect of giloy (Tinospora cordifolia) ethanolic extract supplementation to ram semen extender on sperm abnormality in chilled semen, Journal of Pharmaceutical Innovation, 7, 3361–3365.

    Google Scholar 

  • Banana, H.J.H., Alkhazreji, H.T.A., and Mahdi, Z.A., 2020. Adding different concentrations of pomegranate peels alcoholic extract and its effect on characteristics of Awassi ram semen preserved at cooling, Systematic Reviews in Pharmacy, 11, 1821–1825

    CAS  Google Scholar 

  • Barbas, J., and Mascarenhas, R., 2009. Cryopreservation of domestic animal sperm cells, Cell and tissue banking, 10, 49–62

    Article  CAS  PubMed  Google Scholar 

  • Batellier, F., Vidament, M., Fauquant, J., Duchamp, G., Arnaud, G., Yvon, J., and Magistrini, M., 2001. Advances in cooled semen technology, Animal reproduction science, 68, 181–190

    Article  CAS  PubMed  Google Scholar 

  • Benmoula, A., Allai, L., Badi, A., El Khalil, K., and El Amiri, B., 2018. Effect of extender and storage temperature on sperm motility parameters of liquid ram semen, Rev. Mar. Sci. Agron. Vét, 6, 211–219

    Google Scholar 

  • Bergeron, A., and Manjunath, P., 2006. New insights towards understanding the mechanisms of sperm protection by egg yolk and milk, Molecular reproduction and development, 73, 1338–1344

    Article  CAS  PubMed  Google Scholar 

  • Bergeron, A., Crête, M.-H., Brindle, Y., and Manjunath, P., 2004. Low-density lipoprotein fraction from hen’s egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane, Biology of Reproduction, 70, 708–717

    Article  CAS  PubMed  Google Scholar 

  • Bucak, M.N., Bodu, M., Başpınar, N., Güngör, Ş., İli, P., Acibaeva, B., Topraggaleh, T.R., and Dursun, Ş., 2019. Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen, Cell Journal (Yakhteh), 21, 7–13

    Google Scholar 

  • Bustani, G.S., and Baiee, F.H., 2021. Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders, Veterinary World, 14, 1220–1233

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • De, K., Kumar, D., Sethi, D., Gulyani, R., and Naqvi, S.M.K., 2015. Estrus synchronization and fixed-time artificial insemination in sheep under field conditions of a semi-arid tropical region, Tropical Animal Health and Production, 47, 469–472

    Article  PubMed  Google Scholar 

  • Ducha, N., Budijastuti, W., and Kuswanti, N., 2020. Study of Soya Addition in Tris Base Extender on the Quality of Senduro Goat Spermatozoa and Membrane Integrity on Storage Temperature 4–5° C. Proceedings of the 7th Mathematics, Science, and Computer Science Education International Seminar, MSCEIS 2019, 12 October 2019, Bandung, West Java, Indonesia, 2020, DOI https://doi.org/10.4108/eai.12-10-2019.2296357

  • El-Harairy, M., Abdel-Khalek, A., Khalil, W., Khalifa, E., El-Khateeb, A., and Abdulrhmn, A., 2016. Effect of Aqueous Extracts of Moringa oleifera leaves or Arctium lappa Roots on Lipid Peroxidation and Membrane Integrity of Ram Sperm Preserved at Cool Temperature, Journal of Animal and Poultry Production, 7, 467–473

    Article  Google Scholar 

  • El-Harairy, M., Khalil, W., Khalifa, E., and Saber, A.A., 2018. Effect of propolis ethanolic extract supplementation to ram semen extenders on sperm characteristics, lipid peroxidation and some enzymatic activities in seminal plasma in chilled semen, Journal of Animal and Poultry Production, 9, 235–243

    Article  Google Scholar 

  • Emamverdi, M., Zhandi, M., Zare Shahneh, A., Sharafi, M., and Akbari-Sharif, A., 2013. Optimization of Ram semen cryopreservation using a chemically defined soybean lecithin‐based extender, Reproduction in domestic animals, 48, 899–904

    Article  CAS  PubMed  Google Scholar 

  • Fadl, A.M., Abdelnaby, E.A., and El-Sherbiny, H.R., 2022. INRA82 extender enhances semen quality in ram under cooled and cryopreserved stages, Asian Pacific Journal of Reproduction, 11, 100–104

    Article  CAS  Google Scholar 

  • Foote, R., 2010. The history of artificial insemination: Selected notes and notables, J. Anim. Sci, 80, 1–10

    Article  Google Scholar 

  • Forouzanfar, M., Sharafi, M., Hosseini, S., Ostadhosseini, S., Hajian, M., Hosseini, L., Abedi, P., Nili, N., Rahmani, H., and Nasr-Esfahani, M., 2010. In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen, Theriogenology, 73, 480–487

    Article  CAS  PubMed  Google Scholar 

  • Gheller, S.M.M., Corcini, C.D., Pradieé, J., Rizzoto, G., Junior, T.L., Moreira, F., and Junior, A.S.V., 2018. Gelatin protects ram semen stored for 72 h at 5 C, Small ruminant research, 158, 54–56

    Article  Google Scholar 

  • Gil, J., Rodriguez-Irazoqui, M., Lundeheim, N., Söderquist, L., and Rodrı́guez-Martı́nez, H., 2003. Fertility of ram semen frozen in Bioexcell® and used for cervical artificial insemination, Theriogenology, 59, 1157–1170

    Article  CAS  PubMed  Google Scholar 

  • Gogol, P., Bryla, M., Trzcinska, M., and Bochenek, M., 2019. Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders, Polish Journal of Veterinary Sciences, 22, 177–179

    Article  CAS  PubMed  Google Scholar 

  • Gundogan, M., Avdatek, F., and Yen, D., 2011. Effect of extenders on motility, morphology and osmotic resistance parameters of ram sperm during liquid storage, Rev. Méd. Vét, 162, 546–551

    CAS  Google Scholar 

  • Gündoğan, M., 2009. Short term preservation of ram semen with different extenders, Kafkas Univ Vet Fak Derg, 15, 429–435

    Google Scholar 

  • Hameed, N., Zubair, M., Ahmad, N., Durrani, A.Z., and Khan, M.I.-u.-R., 2023. Effects of extender type and storage time on sperm quality parameters of Kail ram semen stored at 5° C, Tropical Animal Health and Production, 55, 171

    Article  PubMed  Google Scholar 

  • Hegedusova, Z., Štolc, L., Louda, F., Čunát, L., and Vejnar, J., 2012. Effect of different extenders on ram sperm traits during storage, Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 6, 111–116

    Google Scholar 

  • Kasimanickam, R., Kasimanickam, V., Pelzer, K.D., and Dascanio, J.J., 2007. Effect of breed and sperm concentration on the changes in structural, functional and motility parameters of ram-lamb spermatozoa during storage at 4 C, Animal reproduction science, 101, 60–73

  • Kasimanickam, R., Kasimanickam, V., Tibary, A., and Pelzer, K., 2011. Effect of semen extenders on sperm parameters of ram semen during liquid storage at 4 C, Small ruminant research, 99, 208–213

  • Khalifa, M.A., 2017. International Journal of Animal Research (ISSN: 2575–7822) Effect of supplementing ram semen extender with melatonin on oxidative stress indices and physical properties of chilled spermatozoa, Journal of Animal Research, 1, 14

  • Kulaksiz, R., Çebi, Ç., and AKÇAY, E., 2012. The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 C, Turkish Journal of Veterinary & Animal Sciences, 36, 177–182

    Google Scholar 

  • Kumar Patel, G., Haque, N., Madhavatar, M., kumar Chaudhari, A., kumar Patel, D., Bhalakiya, N., Jamnesha, N., Patel, P., and Kumar, R., 2017. Artificial insemination: A tool to improve livestock productivity, Journal of Pharmacognosy and Phytochemistry, 6, 307–313

    Google Scholar 

  • Lopez, A., Söderquist, L., and Rodriguez-Martinez, H., 1999. Sperm viability in ram semen diluted and stored in three different extenders, Acta Veterinaria Scandinavica, 40, 1–9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lopez Saez, A., Ortiz, N., Gallego, L., and Garde, J., 2000. Liquid storage (50 C) of ram semen in different diluents, Archives of andrology, 44, 155–164

    Article  CAS  PubMed  Google Scholar 

  • López-Pérez, A., and Pérez-Clariget, R., 2012. Ram seminal plasma improves pregnancy rates in ewes cervically inseminated with ram semen stored at 5 C for 24 hours, Theriogenology, 77, 395–399

    Article  PubMed  Google Scholar 

  • Madrigali, A., Rota, A., Panzani, D., Castellani, S., Shawahina, M., Hassan, A., Di Iacovo, F., Rossignoli, C., and Camillo, F., 2021. Artificial Insemination in Sheep with Fresh Diluted Semen: Comparison Between Two Different Semen Extenders and Management Protocols, Tropical Animal Science Journal, 44, 255–260

    Article  Google Scholar 

  • Mahdi, A., 2017. Effects of Licorice Extract on Sperm Motility of Chilled Stored Ram Semen, Ibn AL-Haitham Journal For Pure and Applied Science, 23, 34–40

    Google Scholar 

  • Maksimovic, N., Milovanović, A., Barna, T., Petrović, V.C., Pantelić, V., Lazarević, M., and Stojanov, I., 2018. Short-term liquid storage of ram semen in various extenders, South African Journal of Animal Science, 48, 717–723

    Article  CAS  Google Scholar 

  • Mara, L., Accardo, C., Pilichi, S., Dattena, M., Chessa, F., Chessa, B., Branca, A., and Cappai, P., 2005. Benefits of TEMPOL on ram semen motility and in vitro fertility: a preliminary study, Theriogenology, 63, 2243–2253

    Article  CAS  PubMed  Google Scholar 

  • Martı, J., Martı, E., Cebrian-Perez, J., and Muino-Blanco, T., 2003. Survival rate and antioxidant enzyme activity of ram spermatozoa after dilution with different extenders or selection by a dextran swim-up procedure, Theriogenology, 60, 1025–1037

    Article  PubMed  Google Scholar 

  • Martorana, K., Klooster, K., and Meyers, S., 2014. Suprazero cooling rate, rather than freezing rate, determines post thaw quality of rhesus macaque sperm, Theriogenology, 81, 381–388

    Article  PubMed  Google Scholar 

  • Menchaca, A., Pinczak, A., and Queirolo, D., 2018. Storage of ram semen at 5 C: effects of preservation period and timed artificial insemination on pregnancy rate in ewes, Animal Reproduction (AR), 2, 195–198

    Google Scholar 

  • O’Hara, L., Hanrahan, J., Richardson, L., Donovan, A., Fair, S., Evans, A., and Lonergan, P., 2010. Effect of storage duration, storage temperature, and diluent on the viability and fertility of fresh ram sperm, Theriogenology, 73, 541–549

    Article  PubMed  Google Scholar 

  • Palacín, I., Yániz, J., Fantova, E., Blasco, M., Quintín-Casorrán, F., Sevilla-Mur, E., and Santolaria, P., 2012. Factors affecting fertility after cervical insemination with cooled semen in meat sheep, Animal reproduction science, 132, 139–144

    Article  PubMed  Google Scholar 

  • Paulenz, H., Söderquist, L., Pérez-Pé, R., and Berg, K.A., 2002. Effect of different extenders and storage temperatures on sperm viability of liquid ram semen, Theriogenology, 57, 823–836

    Article  PubMed  Google Scholar 

  • Paulenz, H., Ådnøy, T., Fossen, O., and Söderquist, L., 2010. Effect on field fertility of addition of gelatine, different dilution rates and storage times of cooled ram semen after vaginal insemination, Reproduction in domestic animals, 45, 706–710

    CAS  PubMed  Google Scholar 

  • Purdy, P.H., Mocé, E., Stobart, R., Murdoch, W.J., Moss, G.E., Larson, B., Ramsey, S., Graham, J.K., and Blackburn, H.D., 2010. The fertility of ram sperm held for 24 h at 5 C prior to cryopreservation, Animal reproduction science, 118, 231–235

    Article  PubMed  Google Scholar 

  • Quan, G.B., Wu, G.Q., Wang, Y.J., Li, D.J., Ma, Y., and Hong, Q.H., 2016. Effects of the Tris, Tes, or skim milk based extender on in vitro parameters of ram spermatozoa during liquid storage, Small ruminant research, 134, 14–21

    Article  Google Scholar 

  • Rather, H.A., Islam, R., Malik, A.A., and Lone, F.A., 2016. Addition of antioxidants improves quality of ram spermatozoa during preservation at 4 C, Small ruminant research, 141, 24–28

  • Rather, H.A., Islam, R., Malik, A.A., Lone, F.A., and Banday, M.N., 2017. Effect of different extenders for preservation of ram semen at 4 C, Indian Journal of Animal Research, 51, 982–987

    Google Scholar 

  • Rehman, F., Zhao, C., Shah, M., Qureshi, M., and Wang, X., 2013. Semen extenders and artificial insemination in ruminants, Veterinaria, 1, 1–8

    Google Scholar 

  • Rekha, A., Zohara, B., Bari, F., and Alam, M., 2018. Comparisons of commercial Triladyl and locally manufactured extenders for the chilling of semen and their effects on pregnancy rates after transcervical AI in Bangladeshi Indigenous (Ovis aries) sheep, Animal Reproduction (AR), 13, 735–742

    Article  Google Scholar 

  • Salamon, S., and Maxwell, W., 2000. Storage of ram semen, Animal reproduction science, 62, 77–111

    Article  CAS  PubMed  Google Scholar 

  • Santymire, R., Marinari, P., Kreeger, J., Wildt, D., and Howard, J., 2007. Slow cooling prevents cold-induced damage to sperm motility and acrosomal integrity in the black-footed ferret (Mustela nigripes), Reproduction, Fertility and Development, 19, 652–663

  • Sharafi, M., Forouzanfar, M., Hosseini, S.M., Hajian, M., Ostad, H.S., Hosseini, L., Abedi, P., Nili, N., Rahmani, H.R., and Javaheri, A.R., 2009. In vitro comparison of soybean lecithin based-extender with commercially available extender for ram semen cryopreservation, International Journal of Fertility and Sterility, 3, 149–152

    Google Scholar 

  • Souza-Fabjan, J., Oliveira, M., Guimarães, M., Brandão, F., Bartlewski, P., and Fonseca, J., 2023. Non-surgical artificial insemination and embryo recovery as safe tools for genetic preservation in small ruminants, Animal, 17, 100787

    Article  PubMed  Google Scholar 

  • Špaleková, E., Makarevich, A., and Pivko, J., 2011. Effect of caffeine on parameters of ram sperm motility, Slovak Journal of Animal Science, 44, 78–83

    Google Scholar 

  • Sukirman, I., Sukmawati, E., Rasad, S.D., and Solihati, N., 2020. The Influence of Breed and Type of Extender on the Quality of Bull Semen, Animal Production, 21, 64–70

    Article  Google Scholar 

  • Suranagi, M., and Kulkarni, S., 2022. CASA assessment of sperm motility parameters of NARI-Suwarna rams semen after supplementation of semen extenders with almond and olive oil, The Pharma Innovation Journa, 11, 4270–4277

    Google Scholar 

  • Tămâianu, B., Anghel, A.H., Nadolu, D., Ilișiu, E., and Nacu, G., 2021. Monitoring the qualitative parameters of the refrigerated ram semen during non-breeding season, Scientific Papers-Animal Science Series: Lucrări Ştiinţifice - Seria Zootehnie, 75, 104–108

  • Varisli, Ö., Taskin, A., and Akyol, N., 2018. Effects of different extenders and additives on liquid storage of Awassi ram semen, Turkish Journal of Veterinary & Animal Sciences, 42, 230–242

    Article  CAS  Google Scholar 

  • Warr, S., Pini, T., de Graaf, S.P., and Rickard, J.P., 2023. Molecular insights to the sperm–cervix interaction and the consequences for cryopreserved sperm, Biology of Reproduction, 108, 183–196

    Article  PubMed  Google Scholar 

  • Yániz, J., Marti, J., Silvestre, M., Folch, J., Santolaria, P., Alabart, J., and López-Gatius, F., 2005. Effects of solid storage of sheep spermatozoa at 15 C on their survival and penetrating capacity, Theriogenology, 64, 1844–1851

    Article  PubMed  Google Scholar 

  • Yániz, J., Mateos, J., and Santolaria, P., 2011. Zwitterionic buffers preserve ram semen quality more efficiently than TRIS during storage at 15 C, Small ruminant research, 95, 54–60

    Article  Google Scholar 

  • Zarei, M., Rostami, B., Masoumi, R., Sharafi, M., Shahir, M.H., Stear, M., and Catt, S., 2018. Egg yolk enriched with polyunsaturated fatty acids (PUFAs) improves the shelf life of ram semen in liquid storage, Small ruminant research, 166, 87–92

    Article  Google Scholar 

  • Zhang, S., Hu, J., Li, Q., Jiang, Z., and Zhang, X., 2009. The cryoprotective effects of soybean lecithin on boar spermatozoa quality, African Journal of Biotechnology, 8, 6476–6480

    Article  CAS  Google Scholar 

  • Zhang, L., Sohail, T., Yanhu, W., Yan, K., Xuyang, W., Xiaomei, S., and Yongjun, L., 2022. The Effect of Different Storage Temperature on Hu Ram Sperm Parameters, Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, 201–209

    Google Scholar 

  • Zhang, L., Wang, Y., Sun, X., Kang, Y., Sohail, T., Wang, J., and Li, Y., 2023. Effects of Different Diluents on Semen Quality of Hu Ram Stored at 4° C, Animals, 13, 2823

  • Zhao, J., Xiao, G., Zhu, W., Fang, D., Li, N., Han, C., and Gao, Q., 2020. Trehalose addition to a Tris-fructose egg yolk extender on quality of ram sperm preserved at 0° C, Revista Brasileira de Zootecnia, 49, e20200061

  • Zhao, J.-q., Xiao, G.-l., Zhu, W.-l., Fang, D., Li, N., Han, C.-m., and Gao, Q.-h., 2021. Ram semen preserved at 0 C with soybean lecithin Tris-based extender substituted for egg yolk, Animal Bioscience, 34, 192–194

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan

    Nasir Hameed & Muhammad Irfan-ur-Rehman Khan

  2. Department of Veterinary Clinical Sciences, University of Poonch Rawalakot, Azad Kashmir, Pakistan

    Nasir Hameed & Muhammad Zubair

  3. Department of Zoology, University of Poonch Rawalakot, Azad Kashmir, Pakistan

    Shereen Akhter

  4. Faculdade de Veterinária, Universidade Federal Fluminense, Rua Vital Brazil Filho, 64, Niterói, RJ, CEP 24230-340, Brazil

    Joanna Maria Gonçalves Souza-Fabjan

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N.H., M.I.R.K., J.M.G.S.F., and S.A. conceived, designed, and wrote the manuscript. M.Z. and all other authors critically reviewed the manuscript.

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Hameed, N., Akhter, S., Souza-Fabjan, J.M.G. et al. Effects of different extenders, storage temperatures, and antioxidant supplementation on chilled semen quality: a review. Trop Anim Health Prod 56, 85 (2024). https://doi.org/10.1007/s11250-024-03930-2

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