Abstract
In the current study, the role of the ovine IGF2 as a potential candidate gene was investigated as though marker-assisted selection in Chinese Tibetan sheep. The Sanger DNA sequencing method explored five single nucleotide polymorphisms (SNPs) in 5’UTR of the ovine IGF2 gene (C15640T, G15801A, G15870A, C15982G and G15991A) in Chinese Tibetan sheep. The frequencies of four SNPs were within the Hardy–Weinberg Equilibrium (chi-square test) except C15982G. The statistical analysis indicated that the C15640T and G15801A were significantly associated with body height, body length, chest circumference, and body weight (P < 0.05 or P < 0.01). Furthermore, C15982G variant exhibited significant correlation with the body weight (P < 0.01). These findings suggests that the promoter variants of IGF2 gene could be used as a candidate gene through marker-assisted selection for the body weight and body measurement traits in Tibetan sheep breeding program.
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Funding
We are thankful to the Shandong Provincial Natural Science Foundation, China. (ZR2019MC036) and Shaanxi Province, China. (2017JCYJ-4–3) for supporting this research.
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Xianlin Zhao, Jinyun Yan and Hanping Chu conceived the idea, conducted experiment and wrote initial draft of the manuscript; Zhenling Wu and Wendi Li analyzed the data and assisted in the writing of the manuscript; Qing Zhang and Yu Zhang edited and revised the manuscript and assisted in data analysis, Zhaobin Fan helped in revision, supervised the experiments and provided financial assistance.
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Zhao, X., Yan, J., Chu, H. et al. The polymorphism of the ovine insulin like growth factor-2 (IGF2) gene and their associations with growth related traits in Tibetan sheep. Trop Anim Health Prod 56, 19 (2024). https://doi.org/10.1007/s11250-023-03858-z
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DOI: https://doi.org/10.1007/s11250-023-03858-z