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Polymorphism and association study of lactoferrin (LF) gene with milk yield, milk composition, and somatic cell count in Beetal goats

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Abstract

The sequence analysis of PCR product exhibited four novel SNPs in the promoter region of the LF gene at loci g.98T>C, g.143T>A, g.189AC>A, and g.346A>G. Each SNP yielded three genotypes; the genotypes TT (SNP1), AA (SNP3), and GG (SNP4) decreased SCC and increase milk quality traits such as density, protein, and milk yield (P < 0.01). The genotype CC (SNP2) and CA (SNP4) significantly (P < 0.01) decreased the milk quality parameters, while genotypes TC (SNP2) and GG (SNP4) showed significantly (P < 0.01) less SCC and increase lactose % in milk. Furthermore, screening of the LF promoter sequence explored the gain of four TF binding sites at locus g.98T˃C and three TF binding sites at g.346A˃G. However, the loss of four and two TF binding sites was seen at locus g.143T˃A and g.189C˃A, respectively. We can conclude from the present study that the GG, TT, and AA genotype might be utilized as genetic markers in marker-assisted selection for the breed improvement program of Beetal goats.

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Acknowledgements

The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University, Saudi Arabia for supporting this work through the Large Groups Project under grant number R.G.P2/186/44.

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Authors and Affiliations

  1. Department of Livestock Management, Breeding and Genetics, The University of Agriculture, Peshawar, 25130, Pakistan

    Lionel Kinkpe, Rajwali Khan, Syed Muhammad Suhail & Ijaz Ahmad

  2. College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan

    Farhan Anwar Khan

  3. Biology Department, College of Science, King Khalid University, Abha, P.O Box 960, Saudi Arabia

    Amel Ayari-Akkari & Sazada Siddiqui

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  1. Lionel Kinkpe
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Contributions

R.K. and K.L. conceived the idea, performed experiment, and wrote the manuscript. S.M.S., I.A., and F.A.K. assisted in analysis, editing, and revision of the draft manuscript. R.K. supervised overall research and analyzed the data.

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Correspondence to Rajwali Khan.

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Kinkpe, L., Khan, R., Suhail, S.M. et al. Polymorphism and association study of lactoferrin (LF) gene with milk yield, milk composition, and somatic cell count in Beetal goats. Trop Anim Health Prod 55, 415 (2023). https://doi.org/10.1007/s11250-023-03834-7

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