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Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil

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Abstract

Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.

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Acknowledgements

The authors thank the National Agricultural Laboratory (LANAGRO-MG) in Pedro Leopoldo-MG, Brazil and Instituto de Agrobiotecnología y Biología Molecular (IABiMo), Instituto Nacional de Tecnología Agropecuaria (INTA).

Funding

The authors thank the financial support of the National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel Brazil (CAPES)-Financing Code 001.

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Authors and Affiliations

  1. Laboratório de Doenças Infectocontagiosas da Faculdade de Medicina Veterinária da, Universidade Federal de Uberlândia (UFU), Av. Ceará, s/n. 38.4000.902, Uberlândia, MG, Brasil

    Mariana Assunção de Souza, Nadia Grandi Bombonato & Anna Monteiro Correia Lima

  2. Faculdade de Medicina Veterinária do Centro, Universitário de Patos de Minas (UNIPAM), Patos de Minas, MG, Brasil

    Thaís Aline Carolino Lopes & Brendhal Almeida Silva

  3. Laboratório de Tuberculose, Instituto Biológico de São Paulo, São Paulo, Brasil

    Cristina Corsi Dib

  4. Instituto de Agrobiotecnología y Biología Molecular (IABiMo), Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    María Jimena Marfil & Martín José Zumárraga

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  1. Mariana Assunção de Souza
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All listed authors have made substantial contributions to the research design, or the acquisition, analysis, or interpretation of data, and drafting the manuscript or revising it critically. All authors have approved the submitted version.

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de Souza, M.A., Lopes, T.A.C., Silva, B.A. et al. Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil. Trop Anim Health Prod 54, 238 (2022). https://doi.org/10.1007/s11250-022-03243-2

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