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Effects of dietary supplementation with Ampelopsis grossedentata extract on production performance and body health of hens

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Abstract

To investigate the potential of Ampelopsis grossedentata extract used as a feed additive, laying performance, egg quality, yolk cholesterol, plasma biochemical parameters, intestinal histology, and gut microbiota of hens (n = 60) were determined between basal diet (CK) and dietary supplementation with A. grossedentata extract (RT) for 11 weeks. The laying rate in RT group was 6.3 percentage points higher than in CK group together with feed conversion rate decreasing. Significant upregulation of immunoglobulin indexes and downregulation of lipid-related indexes in RT group were also found in comparison with CK group, suggesting that dietary supplementation with A. grossedentata extract benefited in immunity enhancing and blood-fat depressing. Meanwhile, the villus height in duodenum and villus height to crypt depth ratio in duodenum and jejunum of RT group were significantly higher than that of CK group, indicating that dietary supplementation with A. grossedentata extract facilitated nutrient adsorption via intestinal histology changing. Moreover, the richness, diversity, and composition of gut microbiota in RT group significantly altered with a comparison of CK group, including beneficial bacterium and pathogenic bacterium, revealing that dietary supplementation with A. grossedentata extract could modify gut microbiota communities to affect intestinal adsorption and pathogen invasion. In addition, the lipid metabolism-related insulin signing pathway was significantly enriched by gut microbiota in RT group, which were conducive to egg production elevation via facilitating blood lipid amelioration and insulin resistance alleviation. These results provided a basis for A. grossedentata extract served as a feed additive in the hen industry.

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Data availability

The datasets used or analyzed in this study are available from the corresponding author upon request.

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Funding

This study was supported by the National Natural Science Foundation of China (Grant No. 42107020), the GDAS’ Project of Science and Technology Development (2019GDASYL-0502003), the Science and Technology Project of Hunan Province (2020SK2028), and the Traditional Chinese Medicine of Hunan Province (2021238).

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  1. Tao Huang and Xuesong Wang contribute equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangdong Provincial Engineering Research Center for Ambient Mass Spectrometry, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), 100 Xianlie Middle Road, Guangzhou, 510070, China

    Tao Huang, Xuesong Wang, Qiuling Yang & Mijun Peng

  2. National & Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides, Jishou University, Jishou, 427000, China

    Sheng Peng

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  1. Tao Huang
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  4. Sheng Peng
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  5. Mijun Peng
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Contributions

Conceptualization: TH, MP. Methodology: TH. Software: XW. Validation: XW, QY, SP. Formal analysis: TH. Investigation: XW. Resources: SP. Data curation: XW. Writing-original draft preparation: TH. Writing-review and editing: XW. Supervision: MP. Project administration: MP. Funding acquisition: MP. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Xuesong Wang or Mijun Peng.

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Huang, T., Wang, X., Yang, Q. et al. Effects of dietary supplementation with Ampelopsis grossedentata extract on production performance and body health of hens. Trop Anim Health Prod 54, 45 (2022). https://doi.org/10.1007/s11250-022-03044-7

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