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Anthelmintic activity of methanol extract of Dennettia tripetala G. Baker (Annonaceae) fruits against Haemonchus contortus in red Sokoto goats

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Abstract

Haemonchosis is a gastrointestinal parasitic disease of economic importance in ruminants especially sheep and goats. In view of the rising costs of conventional veterinary anthelmintics and the development of resistance by Haemonchus contortus, there is a need to develop alternative ethnoveterinary therapies for the treatment and management of Haemonchosis. This study investigated the anthelmintic activity of Dennettia tripetala G. Baker (Annonaceae) fruits against Haemonchus contortus in red Sokoto goats. The maximum tolerated dose was determined in adult albino rats administered, 3000, 4000, and 5000 mg/kg body weight of the crude methanol extract (CME) fraction, and observed over a period of 48 h for signs of toxicity and mortality. The in vivo anthelmintic activity was evaluated using 20 kids infected with H. contortus and randomly allocated into 5 groups (1, 2, 3, 4, and 5). Kids in groups 1, 2, and 3 were treated with CME at doses of 1250, 2500, and 5000 mg/kg respectively for 3 consecutive days per os. Groups 4 and 5 were treated with albendazole (7.5 mg/kg) once and distilled water (5 ml) respectively and served as treated and untreated controls. Blood samples were collected for haematology. The maximum tolerated dose of the CME of D. tripetala did not produce observable signs of toxicity or death in all the rats given up to 5000 mg/kg. There was significant (P < 0.05) reduction in faecal egg count by CME at doses of 1250 mg/kg (91.6%), 2500 mg/kg (98.5%), and 5000 mg/kg (100%) at day 14 post-treatment. The total plasma protein (TPP) and FAMACHA© scoring values were significantly improved (P < 0.05) in the treated groups. These results indicate that methanol extract and fractions of D. tripetala fruits possess beneficial anthelmintic activity against H. contortus and may be a suitable alternative anthelmintic candidate for the control of haemonchosis in goats.

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© score 5), (III) emaciation and lethargy, and (IV) diarrhoea

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Acknowledgements

The authors are grateful for the technical assistance and facilities provided by the Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, in support of this research.

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Authors and Affiliations

  1. Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria

    Rachel A. Nwosu, Mohammed M. Suleiman, Matthew P. Ameh & Manji A. Shetshak

  2. Small Ruminant Research Programme, National Animal Production Research Institute, Ahmadu Bello University, Zaria, Nigeria

    Hussaina J. Makun

  3. Department of Physiology, Biochemistry, and Pharmacology, Faculty of Veterinary Medicine, University of Jos, Jos, Nigeria

    Isaac O. Akefe

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  1. Rachel A. Nwosu
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  2. Mohammed M. Suleiman
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  6. Isaac O. Akefe
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Contributions

All authors contributed to the study conception and design. RAN, MPA, MAS, and IOA conducted experiments. MMH and HJM contributed new reagents or analytical tools. RAN and IOA analyzed the data. RAN wrote the original manuscript draft. MMH, HJM, and IOA reviewed and edited the manuscript. All authors read and approved the manuscript.

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Correspondence to Isaac O. Akefe.

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Ethical approval for this study with approval number of ABUCAUC/2017/030 was obtained from the Ahmadu Bello University Committee on Animal Use and Care (ABUCAUC), Ahmadu Bello University, Zaria, and has therefore been performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.

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Nwosu, R.A., Suleiman, M.M., Makun, H.J. et al. Anthelmintic activity of methanol extract of Dennettia tripetala G. Baker (Annonaceae) fruits against Haemonchus contortus in red Sokoto goats. Trop Anim Health Prod 54, 2 (2022). https://doi.org/10.1007/s11250-021-03005-6

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