Abstract
The aim of this study was to evaluate the effects of quinoa seed–based diets with phytase and protease enzymes, individually or in combination, on the productive performance, internal organs, microbiota activity, and intestinal morphology of broiler chickens. A total of 250 1-day-old broiler chicks (ROSS 308) were randomly distributed into five treatments with each five replicate pens of 10 birds in a 42-day trial. Dietary treatments were a conventional diet (CC) based on a corn-soybean meal or an experimental diet (EC) which contained 150 g/kg of quinoa seed. Other treatments were EC diet supplemented with 500 FTU/kg phytase (EPH), 0.2 g/kg protease (EPR) enzymes, or their combination (EPPC). At the end of the trial, the feed intake of the EPH broilers was higher than those of the EC and EPPC birds (P < 0.05). Meanwhile, broiler chickens fed the EPH and EPPC diets had higher body weight gain compared with those of the birds fed the other diets (P < 0.05). The EC and EPPC broilers consumed less feed than the EPH birds (P < 0.05), while CC and EPR birds were intermediate. The EC group had the highest feed conversion ratio among all groups (P < 0.05). The EPPC group had the best feed conversion ratio compared with other groups except for the EPH group (P < 0.05). The highest relative weight of the bursa and spleen was observed in EPPC and CC groups, respectively. The pancreas had the lowest weight in the broilers fed protease-supplemented diet. An increase in villus length and villus width was observed in birds fed EPH and EC diets, respectively (P < 0.05). The ratio of VL to crypt depth was greater in the phytase-supplemented group (P < 0.05). Inclusion of phytase or phytase + protease to QS diet increased the cecal Lactobacillus population, while the count of Coliforms decreased in EPR and EPPC groups (P < 0.05). It is concluded that addition of phytase and protease enzymes to QS-based diet may have beneficial effects on the growth performance, gut bacteria ecology, and intestinal morphology of broiler chickens.
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All data generated and analyzed during this study are included in this published article.
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The study was conducted at a commercial poultry farm (Qaemshahr, Mazandaran Province, north of Iran). All broiler chickens’ care and use procedures were approved by the Department of Animal Science, Islamic Azad University (Qaemshar branch, Qaemshahr, Iran).
References
Abd El. Hack ME, Alagawany M, Arif M, Emam M, Saeed M, Arain MA, Siyal FA, Patra A, Elnesr SS, Khan RU (2018) The use of microbial phytase as a feed additive in poultry nutrition - A review Annals of Animal Science 18(3): 639–658
Angel C, Saylor W, Vieira S, Ward N (2011) Effects of a monocomponent protease on performance and protein utilization in 7-to 22-day-old broiler chickens Poultry science 90:2281–2286
Aydin A, Pekel A, Issa G, Demirel G, Patterson P (2010) Effects of dietary copper, citric acid, and microbial phytase on digesta pH and ileal and carcass microbiota of broiler chickens fed a low available phosphorus diet Journal of Applied Poultry Research 19:422–431
Borda-Molina D, Seifert J, Camarinha-Silva A (2018) Current perspectives of the chicken gastrointestinal tract and its microbiome Computational and structural biotechnology journal 16:131-139
Borda-Molina D, Vital M, Sommerfeld V, Rodehutscord M, Camarinha-Silva A (2016) Insights into broilers’ gut microbiota fed with phosphorus, calcium, and phytase supplemented diets Frontiers in microbiology 7:2033
Borda-Molina D, Zuber T, Siegert W, Camarinha-Silva A, Feuerstein D, Rodehutscord M (2019) Effects of protease and phytase supplements on small intestinal microbiota and amino acid digestibility in broiler chickens Poultry science 98:2906-2918
Cowieson A, Lu H, Ajuwon K, Knap I, Adeola O (2017) Interactive effects of dietary protein source and exogenous protease on growth performance, immune competence and jejunal health of broiler chickens Animal Production Science 57:252–261
Eftekhari A, Rezaeipour V, Abdullahpour R (2015) Effects of acidified drinking water on performance, carcass, immune response, jejunum morphology, and microbiota activity of broiler chickens fed diets containing graded levels of threonine Livestock Science 180:158–163 https://doi.org/10.1016/j.livsci.2015.07.010
Ghazi S, Rooke J, Galbraith H, Bedford M (2002) The potential for the improvement of the nutritive value of soya-bean meal by different proteases in broiler chicks and broiler cockerels British poultry science 43:70-77
Ghosh A, Mandal GP, Roy A, Patra AK (2016) Effects of supplementation of manganese with or without phytase on growth performance, carcass traits, muscle and tibia composition, and immunity in broiler chickens Livestock Science 91: 80–85
Hajiaghapour M, Rezaeipour V (2018) Comparison of two herbal essential oils, probiotic, and mannan-oligosaccharides on egg production, hatchability, serum metabolites, intestinal morphology, and microbiota activity of quail breeders Livestock Science 210:93–98 https://doi.org/10.1016/j.livsci.2018.02.007
Hazrati S, Rezaeipour V, Asadzadeh S (2019) Effects of phytogenic feed additives, probiotic and mannan-oligosaccharides on performance, blood metabolites, meat quality, intestinal morphology, and microbial population of Japanese quail British Poultry Science:1–8 doi:https://doi.org/10.1080/00071668.2019.1686122
Hezaveh MSS, Ghasemi HA, Hajkhodadadi I, Moradi MH (2020) Single and combined effects of phytase and citric acid on growth performance, nutrient digestibility, bone characteristics, intestinal morphology, and blood components in meat-type quails fed low-phosphorous diets Animal Feed Science and Technology 269:114677
Jacobsen E, Skadhauge B, Jacobsen S-E (1997) Effect of dietary inclusion of quinoa on broiler growth performance Animal Feed Science and Technology 65:5-14
Kamel N, Ragaa M, El-Banna R, Mohamed F (2015) Effects of a monocomponent protease on performance parameters and protein digestibility in broiler chickens Agriculture and Agricultural Science Procedia 6:216-225
Kolbadinejad A, Rezaeipour V (2020) Efficacy of ajwain (Trachyspermum ammi L.) seed at graded levels of dietary threonine on growth performance, serum metabolites, intestinal morphology and microbial population in broiler chickens Journal of animal physiology and animal nutrition 104:1333–1342
Law FL, Zulkifli I, Soleimani AF, Liang JB, Awad EA (2018) The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment Asian-Australasian journal of animal sciences 31:1291
Morvay AA, Decun M, Sala C, Morar A, Galbenu-Morvay PL (2011) The Ability of Bacillus licheniformis Protease to Remove Bacillus cereus and Pseudomonas aeruginosa biofilm Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca Veterinary Medicine 68
Nokandi A, Hosseini-Vashan SJ, Ghiasi SE, Naeimipour-Yonesi H (2021) Growth performance, blood indices, immune response, nutrient digestibility and intestinal morphology of broiler chickens fed processed quinoa seed (Chenopodium quinoa Willd) Iranian Journal of animal Science 51:285–297
Oboh G, Elusiyan C (2007) Changes in the nutrient and anti-nutrient content of micro-fungi fermented cassava flour produced from low-and medium-cyanide variety of cassava tubers African Journal of Biotechnology 6
Pacheco W, Stark C, Ferket P, Brake J (2014) Effects of trypsin inhibitor and particle size of expeller-extracted soybean meal on broiler live performance and weight of gizzard and pancreas Poultry Science 93:2245-2252
Ptak A, Bedford MR, Świątkiewicz S, Żyła K, Józefiak D (2015) Phytase modulates ileal microbiota and enhances growth performance of the broiler chickens PloS one 10:e0119770
Rezaeipour V, Barsalani A, Abdullahpour R (2016) Effects of phytase supplementation on growth performance, jejunum morphology, liver health, and serum metabolites of Japanese quails fed sesame (Sesamum indicum) meal-based diets containing graded levels of protein Tropical Animal Health and Production 48:1141–1146 https://doi.org/10.1007/s11250-016-1066-x
Roofchaei A, Rezaeipour V, Vatandour S, Zaefarian F (2019) Influence of dietary carbohydrases, individually or in combination with phytase or an acidifier, on performance, gut morphology and microbial population in broiler chickens fed a wheat-based diet Animal Nutrition 5:63–67
SAS (1999) SAS Statistics User’s Guide. Statistical Analytical System, fifth revised ed. SAS Institute Inc, Carry, NC.
Selle PH, Cowieson AJ, Ravindran V (2009a) Consequences of calcium interactions with phytate and phytase for poultry and pigs Livestock Science 124:126-141
Selle PH, Ravindran V, Partridge GG (2009b) Beneficial effects of xylanase and/or phytase inclusions on ileal amino acid digestibility, energy utilisation, mineral retention and growth performance in wheat-based broiler diets Animal Feed Science and Technology 153:303–313 https://doi.org/10.1016/j.anifeedsci.2009.06.011
Smulikowska S, Czerwiński J, Mieczkowska A (2010) Effect of an organic acid blend and phytase added to a rapeseed cake‐containing diet on performance, intestinal morphology, caecal microflora activity and thyroid status of broiler chickens Journal of Animal Physiology and Animal Nutrition 94:15–23
Walk C, Bedford M, McElroy A (2012) Influence of limestone and phytase on broiler performance, gastrointestinal pH, and apparent ileal nutrient digestibility Poultry Science 91:1371–1378
Walters H, Coelho M, Coufal C, Lee J (2019) Effects of increasing phytase inclusion levels on broiler performance, nutrient digestibility, and bone mineralization in low-phosphorus diets Journal of Applied Poultry Research 28:1210–1225
Wang X, Farnell Y, Peebles E, Kiess A, Wamsley K, Zhai W (2016) Effects of prebiotics, probiotics, and their combination on growth performance, small intestine morphology, and resident Lactobacillus of male broilers Poultry science 95:1332–1340
Wu G (2015) Nutritional properties of quinoa. Quinoa: Improvement and sustainable production:193–210
Wu Y, Ravindran V, Thomas D, Birtles M, Hendriks W (2004) Influence of phytase and xylanase, individually or in combination, on performance, apparent metabolisable energy, digestive tract measurements and gut morphology in broilers fed wheat-based diets containing adequate level of phosphorus British poultry science 45:76–84
Zaefarian F, Romero L, Ravindran V (2013) Influence of a microbial phytase on the performance and the utilisation of energy, crude protein and fatty acids of young broilers fed on phosphorus-adequate maize-and wheat-based diets British poultry science 54:653–660
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All authors contributed to the study conception and design. Data collection was done by MY Asnaashari. Data analysis and manuscript preparation were performed by MA Jafari and M Irani. All authors edited, read, and approved the final manuscript.
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Amiri, M.Y.A., Jafari, M.A. & Irani, M. Growth performance, internal organ traits, intestinal morphology, and microbial population of broiler chickens fed quinoa seed–based diets with phytase or protease supplements and their combination. Trop Anim Health Prod 53, 535 (2021). https://doi.org/10.1007/s11250-021-02980-0
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DOI: https://doi.org/10.1007/s11250-021-02980-0