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Haematological profiles of Indonesian fat-tailed sheep under different physiological conditions

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Abstract

The aim of this research was to evaluate the effect of sex, age, feed and physiological conditions on the haematological profiles of Indonesian fat-tailed sheep. A total of 32 healthy Indonesian fat-tailed sheep in different physiological states (i.e. suckling lambs/weaners aged 1–5 months, postweaning lambs aged 6–8 months, lactating ewes, rams, and pregnant ewes) were used in this study. Blood samples were collected from the jugular vein and then transferred into tubes for haematological analysis. The results revealed the following: RBCs, 7.09 ± 0.52 million µL−1; HCT, 46.76 ± 4.30%; Hb, 9.83 ± 0.57 g dL−1; MCV, 69.88 ± 5.48 fL; MCH, 14.94 ± 0.71 pg; MCHC, 24.29 ± 1.36 g dL−1; WBCs, 5.60 ± 1.24 thousand µL−1; percentage of neutrophils, 58.30 ± 7.31%; percentage of lymphocytes, 41.63 ± 7.31%; and N/L ratio, 0.18 ± 0.05. The physiological status of the sheep affected RBCs, Hb, HCT and MCH (P < 0.05). The sex, age, feed and physiological status of the animals should be considered in the interpretation of haematological profiles in Indonesian fat-tailed sheep.

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References

  • Ahmadi-hamedani, M., Ghazvinian, K., Atyabi, N., Khanalizadeh, P., Masoum, M.A., Ghodrati, M.S., 2016. Hematological reference values of healthy adult Sangsari sheep (Iranian fat-tailed sheep) estimated by Reference Value Advisor. Comparative Clinical Pathologyl 25, 459–464. https://doi.org/10.1007/s00580-015-2211-z

    Article  Google Scholar 

  • Alonso, A.J., Teresa, R., García, M., González, J.R., Vallejo, M., 1997. The Effects of Age and Reproductive Status on Serum and Blood Parameters in Merino Breed Sheep. Journal of Veterinary Medicine Series A 44, 223–231. https://doi.org/10.1111/j.1439-0442.1997.tb01104.x

    Article  PubMed  CAS  Google Scholar 

  • AOAC, 2000. Association of Official Analytical Chemists: Official Methods of Analysis. Washington DC, USA.

  • Azimzadeh, K., Javadi, A., 2020. Serum Biochemistry and Haematology of Iranian Red Sheep (Ovis orientalis gmelini) in Sorkhabad Protected Area, Zanjan, Iran: Comparison with Age and Sex. Iranian Journal of Veterinary Medicine 14, 76–84. https://doi.org/10.22059/ijvm.2019.239317.1004840

  • Barsila, S.R., Bhatt, K., Devkota, B., Devkota, N.R., 2020. Haematological changes in transhumant Baruwal sheep (Ovis aries) grazing in the western Himalayan mountains in Nepal. Pastoralism 10, 1–10. https://doi.org/10.1186/s13570-019-0156-6

    Article  Google Scholar 

  • Bezerra, L.R., Oliveira, W.D.C., Silva, T.P.D., Torreão, J.N.C., Marques, C.A.T., Araújo, M.J., Oliveira, R.L., 2017. Comparative hematological analysis of Morada Nova and Santa Inês ewes in all reproductive stages. Pesquisa Veterinária Brasileira  37, 408–414. https://doi.org/10.1590/s0100-736x2017000400017

    Article  Google Scholar 

  • Borjesson, D.L., Christopher, M.M., Boyce, W.M., 2000. Biochemical and hematologic reference intervals for free-ranging desert bighorn sheep. Journal of Wildlife Diseases 36, 294–300. https://doi.org/10.7589/0090-3558-36.2.294

  • Bórnez, R., Linares, M.B., Vergara, H., 2009. Haematological, hormonal and biochemical blood parameters in lamb: Effect of age and blood sampling time. Livestock Science 121, 200–206. https://doi.org/10.1016/j.livsci.2008.06.009

    Article  Google Scholar 

  • Budisatria, I.G.S., Udo, H.M.J., Eilers, C.H.A.M., Baliarti, E., van der Zijpp, A.J., 2010. Preferences for sheep or goats in Indonesia. Small Ruminant Research 88, 16–22. https://doi.org/10.1016/j.smallrumres.2009.11.002

    Article  Google Scholar 

  • Byers, S.R., Kramer, W., 2010. Normal Hematology of Sheep and Goats, in: Schalm’s Veterinary Hematology Editors Douglas J. Weiss, K. Jane Wardrop. Wiley Blackwell, pp. 836–842.

  • Clinical and Laboratory Standards Institute:, 2008. Defining, Establishing, and Verifying Reference Response in the Clinical Laboratory; Approved Guideline. 3rd ed. CLSI document C28-A3c. Wayne, P. A., Clinical and Laboratory Standards Institute 28, pp. 3

  • Daulay, A.R., Ermansyah, L., 2020. Statistik Peternakan Dan Kesehatan Hewan 2020 (Livestock and Animal Health Statistics 2020). Direktorat Jenderal Peternakan dan Kesehatan Hewan. Kementerian Pertanian RI, Jakarta. http://ditjenpkh.pertanian.go.id/buku-statistik-peternakan-dan-kesehatan-hewan-tahun-2020

  • Dias, I.R., Viegas, C.A., Silva, A.M., Pereira, H.F., Sousa, C.P., Carvalho, P.P., Cabrita, A.S., Fontes, P.J., Silva, S.R., Azevedo, J.M.T., 2010. Haematological and biochemical parameters in Churra-da-Terra-Quente ewes from the northeast of Portugal. Arquivo Brasileiro de Medicina Veterináriae Zootecnia 62, 265–272. https://doi.org/10.1590/S0102-09352010000200004

    Article  CAS  Google Scholar 

  • El-Sherif, M.M.A., Assad, F., 2001. Changes in some blood constituents of Barki ewes during pregnancy and lactation under semi arid conditions. Small Ruminant Research 40, 269–277. https://doi.org/10.1016/S0921-4488(01)00174-2

    Article  PubMed  Google Scholar 

  • Hawkey, C.M., Hart, M.G., Fitzgerald, A.K., 1983. Age related haematological changes in captive Barbary sheep (Ammotragus lervia). Research in Veterinary Sciencei 35, 118–119.

    Article  CAS  Google Scholar 

  • Islam, S., Rahman, M., Ferdous, J., Hossain, M., Hassan, M., Islam, A., 2018. Hematological reference values for healthy fat-tailed sheep (Dhumba) in Bangladesh. Journal of Advanced Veterinary and Animal Research 5, 481–484. https://doi.org/10.5455/javar.2018.e302

    Article  PubMed  PubMed Central  Google Scholar 

  • Jackson, P., Cockcroft, P., 2002. Clinical Examination of Farm Animals. Blackwell Publishing.

  • Jain, N., 1993. Essentials of veterinary haematology. Lea and Febiger, Philadelphia, USA.

    Google Scholar 

  • Kiran, S., Bhutta, A.M., Khan, B.A., Durrani, S., Ali, M., Ali, M., Iqbal, F., 2012. Effect of age and gender on some blood biochemical parameters of apparently healthy small ruminants from Southern Punjab in Pakistan. Asian Pacific Journal of Tropical Biomedicine 2, 304–306. https://doi.org/10.1016/S2221-1691(12)60028-8

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Krajnicakova, M., Bekeova, E., Kacmarik, J., Valocky, I., Hendrichovsky, V., Maracek, I., 1997. Comparison of selected hematological parameters in September-and February-lambing of Slovak Merino sheep. Small Ruminant Research 26, 131–135. https://doi.org/10.1016/S0921-4488(96)00986-8

    Article  Google Scholar 

  • Kramer, J.W., 2000. Normal hematology of cattle, Sheep, and Goats In: Schalm’s Veterinary Hematology (Kramer, B. F., J. G, Zinkl, N. C. Jain, Eds.). Lippincot Williams & Wilkins, Baltimore, pp. 1057–1084.

  • Lepherd, M., Canfield, P., Hunt, G., Bosward, K., 2009. Haematological, biochemical and selected acute phase protein reference intervals for weaned female Merino lambs. Australian Veterinary Journal 87, 5–11. https://doi.org/10.1111/j.1751-0813.2008.00382.x

    Article  PubMed  CAS  Google Scholar 

  • Madureira, K.M., Gomes, V., Barcelos, B., Zani, B.H., Shecaira, C. de L., Costa, C.R., Benesi, F.J., 2013. Parâmetros hematológicos e bioquímicos de ovinos da raça Dorper. Semina: Ciências Agrárias 34, 811–816. https://doi.org/10.5433/1679-0359.2013v34n2p811

  • Mašek, T., Konjević, D., Severin, K., Janicki, Z., Grubešić, M., Krapinec, K., Bojanc, J., Mikulec, Ž., Slavica, A., 2009. Hematology and serum biochemistry of European mouflon (Ovis orientalis musimon) in Croatia. European Journal of Wildlife Research 55, 561–566. https://doi.org/10.1007/s10344-009-0276-1

    Article  Google Scholar 

  • Mitruka, B., Rawnsley, H., 1977. Clinical, Biochemical and Haematological refrence values in norma experimental animals. Masson Publishing USA Inc, New York.

  • Mohammed, A., Campbell, M., Youssef, F.G., 2014. Serum Copper and Haematological Values of Sheep of Different Physiological Stages in the Dry and Wet Seasons of Central Trinidad. Veterinary Medicine International 2014, 1–7. https://doi.org/10.1155/2014/972074

    Article  CAS  Google Scholar 

  • Mohapatra, A., Shinde, A.K., 2018. Fat-Tailed sheep-An important sheep genetic resource for meat production in tropical countries: An overview. Indian Journal of Small Ruminants 24, 1–17. https://doi.org/10.5958/0973-9718.2018.00020.X

    Article  Google Scholar 

  • Mostaghni, K., Badiei, K., Emadi, M., 2005. Haematology and serum biochemistry of captive wild sheep (Ovis orientalis esphahanica) in Iran. Comparative Clinical Pathology 13, 158–161. https://doi.org/10.1007/s00580-004-0526-2

    Article  Google Scholar 

  • Murphy, W.G., 2014. The sex difference in haemoglobin levels in adults — Mechanisms, causes, and consequences. Blood Reviews 28, 41–47. https://doi.org/10.1016/j.blre.2013.12.003

    Article  PubMed  CAS  Google Scholar 

  • Oliveira, W.D.C. de, Dias e Silva, T.P., Araújo, M.J. de, Edvan, R.L., Oliveira, R.L., Bezerra, L.R., 2019. Changes in hematological biomarkers of Nellore cows at different reproductive stages. Acta Scientiarum. Animal Sciences 41, 1–7. https://doi.org/10.4025/actascianimsci.v41i1.45725

  • Pascual-Alonso, M., Miranda-de la Lama, G.C., Aguayo-Ulloa, L., Villarroel, M., Mitchell, M., María, G.A., 2017. Thermophysiological, haematological, biochemical and behavioural stress responses of sheep transported on road. Journal of Animal Physiology and Animal Nutrition 101, 541–551. https://doi.org/10.1111/jpn.12455

    Article  PubMed  CAS  Google Scholar 

  • Radostits, O.M., Blood, D., C., Gay, C.C., 2002. Veterinary Medicine: A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses, 8th Ed. ed. Bailliere Tindall, Britain.

  • Rahman, Md. K., Islam, S., Ferdous, J., Uddin, Md. H., Hossain, M.B., Hassan, M.M., Islam, A., 2018. Determination of hematological and serum biochemical reference values for indigenous sheep (Ovies aries) in Dhaka and Chittagong Districts of Bangladesh. Veterinary World 11, 1089–1093. https://doi.org/10.14202/vetworld.2018.1089-1093

  • Roubies, N., Panousis, N., Fytianou, A., Katsoulos, P.D., Giadinis, N., Karatzias, H., 2006. Effects of Age and Reproductive Stage on Certain Serum Biochemical Parameters of Chios Sheep Under Greek Rearing Conditions. Journal of Veterinary Medicine Series A 53, 277–281. https://doi.org/10.1111/j.1439-0442.2006.00832.x

    Article  PubMed  CAS  Google Scholar 

  • Sharma, A., Kumar, P., Singh, M., Vasishta, N.K., 2015. Haemato-biochemical and endocrine profiling of north western Himalayan Gaddi sheep during various physiological/reproductive phases. Open Veterinary Journal 5, 103–107.

    PubMed  PubMed Central  CAS  Google Scholar 

  • Shek Vugrovečki, A., Vojta, A., Šimpraga, M., 2017. Establishing reference intervals for haematological and biochemical blood variables in Lika pramenka sheep. Veterinarski arhiv 87, 487–499. https://doi.org/10.24099/vet.arhiv.160314

  • Šimpraga, M., Šmuc, T., Matanović, K., Radin, L., Shek-Vugrovečki, A., Ljubičić, I., Vojta, A., 2013. Reference intervals for organically raised sheep: Effects of breed, location and season on hematological and biochemical parameters. Small Ruminant Research 112, 1–6. https://doi.org/10.1016/j.smallrumres.2012.11.032

    Article  Google Scholar 

  • Šoch, M., Brouček, J., Šrejberová, P., 2011. Hematology and blood microelements of sheep in south Bohemia. Biologia 66, 181–186. https://doi.org/10.2478/s11756-010-0150-3

    Article  CAS  Google Scholar 

  • Sodiq, A., Tawfik, E.S., 2004. Productivity and Breeding Strategies of Sheep in Indonesia: A Review. Journal of Agriculture and Rural Development in the Tropics and Subtropics 105, 71–82.

    Google Scholar 

  • Sousa, R., Chaves, D., Barrêto-Júnior, R., Sousa, I.K., Soares, H., Barros, I., Minervino, A.H., Ortolani, E., 2012. Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion. BMC Veterinary Researchs 8, 61. https://doi.org/10.1186/1746-6148-8-61

    Article  PubMed  PubMed Central  Google Scholar 

  • Tibbo, M., Aragaw, K., Abunna, F., Woldemeskel, M., Deressa, A., Dechassa, M.L., Rege, J.E.O., 2005. Factors affecting haematological profiles in three indigenous Ethiopian sheep breeds. Comparative Clinical Pathology 13, 119–127. https://doi.org/10.1007/s00580-004-0525-3

    Article  Google Scholar 

  • Udo, H.M.J., Budisatria, I.G.S., 2011. Fat-tailed sheep in Indonesia; an essential resource for smallholders. Tropical Animal Health and Production 43, 1411–1418. https://doi.org/10.1007/s11250-011-9872-7

    Article  PubMed  PubMed Central  Google Scholar 

  • Vengušt, G., Vengušt, A., Bidovec, A., Klinkon, M., 2002. Some Haematological Values of Farmed Fallow Deer (Dama dama). Comparative Clinical Pathology 11, 223–228. https://doi.org/10.1007/s005800200023

    Article  Google Scholar 

  • Waziri, M.A., Ribadu, A.Y., Sivachelvan, N., 2010. Changes in the serum proteins, hematological and some serum biochemical profifi les in the gestation period in the Sahel goats. Veterinarski arhiv 80, 215–224.

    CAS  Google Scholar 

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Acknowledgements

The authors also thank Akhmad Abror As Sidiqi for his technical assistance with the blood sampling.

Funding

This research was funded by the Faculty of Veterinary Medicine, Gadjah Mada University, with grant No. 1011/J01.1.22/HK4/2018, March 15, 2018.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

    Sarmin, Amelia Hana, Pudji Astuti & Claude Mona Airin

  2. Agency of Agriculture, Food and Fisheries of Sleman Regency of Yogyakarta, Yogyakarta, Indonesia

    Sugi Winarsih

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  1. Sarmin
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  2. Sugi Winarsih
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  3. Amelia Hana
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  4. Pudji Astuti
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  5. Claude Mona Airin
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Contributions

All authors contributed to this study. Preparation of the material, collection of the data and analysis were conducted by Sarmin, Amelia Hana, Pudji Astuti, Claude Mona Airin and Sugi Winarsih. Sarmin wrote the first draft, and all authors contributed to completing the manuscript and approved the final manuscript.

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Correspondence to Sarmin.

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The Ethics Committee of Ethical Clearance for Preclinical Research, Integrated Research and Laboratory Testing at Gadjah Mada University, Yogyakarta, Indonesia, reviewed and approved all of the protocols in this study under licence number 00031/04/LPPT/IV/2018.

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Sarmin, Winarsih, S., Hana, A. et al. Haematological profiles of Indonesian fat-tailed sheep under different physiological conditions. Trop Anim Health Prod 53, 523 (2021). https://doi.org/10.1007/s11250-021-02961-3

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