Skip to main content
Springer Nature Link
Log in

Nutritional and physiological responses of broiler chicken to the dietary supplementation of Moringa oleifera aqueous leaf extract and ascorbic acid in tropics

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

This study was intended to determine the comparative effects of Moringa oleifera aqueous leaf extract (MOALE) and ascorbic acid supplementation in the drinking water on growth performance, haemato-biochemical profile, antioxidant status and immune response of broiler chickens under tropical climate. All 135-day-old broiler chicks were divided into 3 different treatment groups. T0 served as control, T1 were fed basal ration with MOALE (90 ml/L drinking water), and T2 offered basal ration with ascorbic acid (15 mg/L drinking water) for 35-day experiment. All the standard managemental practices were followed during the experimental period. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of MOALE was calculated with reference to different ascorbic acid concentration as standard. The antioxidant activity percentage of MOALE (90 µl) was 93.89%, and ascorbic acid (15 µg) was 98.76%, respectively, which was nearer to cent percent. Therefore, 90 ml MOALE and 15 mg ascorbic acid per litre drinking water, respectively, were used for supplementation in broiler chicken. The growth performance and feed efficiency were better in MOALE group followed by ascorbic acid supplemented birds without affecting the metabolism of nutrients. However, 10.71% better FCR was noted in MOALE supplemented group followed by ascorbic acid group (6.28%) in comparison with control. Most of the haemato-biochemical profiles were unaffected by the treatment except creatinine, while antioxidant profile was improved in the treatment group. Immunity status of broiler chicken against NDV was enhanced in both treatment groups; however, maximum profit is obtained in MOALE group followed by ascorbic acid supplemented birds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

Data Availability

Not applicable.

Code availability

Not applicable.

References

  • Abousekken MSM. 2015. Performance, immune response and carcass quality of broilers fed low protein diets contained either Moringa oleifera leaves meal or its extract. Journal of American Science, 11, 6, 153-164.

    Google Scholar 

  • Abu Hafsa SH, Ibrahim SA, Eid YZ, Hassan AA. 2020. Effect of dietary Moringa oleifera leaves on the performance, ileal microbiota and antioxidative status of broiler chickens. Journal of Animal Physiology and Animal Nutrition, 104, 2, 529-538.

    Article  CAS  PubMed  Google Scholar 

  • Aderinola OA, Rafiu TA, Akinwumi AO, Alabi TA, Adeagbo OA. 2013. Utilization of Moringa oleifera leaf as feed supplement in broiler diet. International Journal of Food, Agriculture and Veterinary Sciences, 3, 94-102.

    Google Scholar 

  • Agashe JL, Manwar SJ, Khose KK, Wade MR. 2017. Effect of supplementation of Moringa oleifera leaf powder on growth performance of broilers. Journal of Poultry Science and Technology 3, 5, 28-34.

    Google Scholar 

  • Ahmed D, Khan MM, Saeed R. 2015. Comparative analysis of phenolics, flavonoids, and antioxidant and antibacterial potential of methanolic, hexanic and aqueous extracts from Adiantum caudatum leaves. Antioxidants, 4, 394-409.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Alabi OJ, Malik AD, Ngambi JW, Obaje P, Ojo BK. 2017. Effect of aqueous Moringa oleifera (Lam) leaf extracts on growth performance and carcass characteristics of Hubbard broiler chicken Brazilian Journal of Poultry Science, 19, 2, 273-280.

  • Allam H, Abdelazem AM, Farag HS, Hamed A. 2016. Some haemato-biochemical, bacteriological and pathological effect of Moringa oleifera leaf extract in broiler chicken. International Journal of Basic and Applied Sciences 5, 2, 99-104.

    Article  CAS  Google Scholar 

  • Allen WH, Lancasta JE, Toch B. 1978. Newcastle disease vaccine, their production and uses. FAO. Rome. 57–62.

  • AOAC. 2005. Official methods of analysis. 18th ed. Association of Official Analytical Chemists, Washington, D.C.

    Google Scholar 

  • Bhatti N, Hussain Z, Mukhtar M, Ali A, Imran M. 2016. Effects of vitamins E and C supplementation on the immune response of broiler chicks. Journal of Antivirals and Antiretrovirals, 8, 4, 151-154.

    Article  Google Scholar 

  • BIS. 2007. Indian standard, poultry feeds specifications. Bureau of Indian Standards, 5th Review. Manak Bhawan, 9, Bahadur Shah Zafar Marg, New Delhi, India.

  • Campbell, TW. 1995. Avian haematology and cytology. Iowa State University Press, Amess Iowa.

    Google Scholar 

  • Cohen G, Dembiec D, Marcus J. 1970. Measurement of catalase activity in tissues extracts. Animal Biochemistry, 34, 30-38.

    Article  CAS  Google Scholar 

  • Cui YM, Wang JLW, Zhang HJ, Wu SG, Qi GH. 2018. Effect of dietary supplementation with Moringa oleifera leaf on performance, meat quality, and oxidative stability of meat in broilers. Poultry Science, 97, 8, 2836-2844. doi: https://doi.org/10.3382/ps/pey122.

    Article  CAS  PubMed  Google Scholar 

  • Divya, Mandal AB, Biswas A, Yadav AS, Biswas AK. 2014. Effect of dietary Moringa oleifera leaves powder on growth performance, blood chemistry, meat quality and gut microflora of broiler chicks. Animal Nutrition and Feed Technology, 14, 349-357.

    Article  Google Scholar 

  • Drabkin DL. 1932. Haemoglobin Drabkin colorimetric method. Journal of Biological Chemistry, 98, 719.

    Article  CAS  Google Scholar 

  • Esiegwu AC. 2019. The supplementary effect of Moringa oleifera leaf water on performance and blood indices of finisher broilers. Direct Research Journal of Veterinary Medicine and Animal Science, 4, 4, 27-32,

    Google Scholar 

  • Faluyi OB, Agbede JO. 2018. Immuno-modulatory activity of aqueous leaf extract of Moringa oleifera in broiler chickens. International Journal of Environment, Agriculture and Biotechnology, 3, 1, 49-54.

    Article  Google Scholar 

  • Gan L, Fan H, Nie W, Guo Y. 2018. Ascorbic acid synthesis and transportation capacity in old laying hens and the effects of dietary supplementation with ascorbic acid. Journal of Animal Science and Biotechnology, 9, 1, 71. doi: https://doi.org/10.1186/s40104-018-0284-7.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jain G, Neeraj, Pandey R. 2018. Effect of vitamin C on growth performance of caged broilers. Advances in Bioresearch, 9, 2, 178-181.

    Google Scholar 

  • Jung IL. 2014. Soluble extract from Moringa oleifera leaves with a new anticancer activity. PLoS One, 9, 4, e-95492. https://doi.org/10.1371/journal.pone.0095492.

  • Khan W, Parveen R, Chester K, Parveen S, Ahmad S. 2017. Hypoglycaemic potential of aqueous extract of Moringa oleifera leaf and in-vivo GC-MS metabolomics. Frontiers in Pharmacology, 8, 577.

    Article  PubMed  PubMed Central  Google Scholar 

  • Kumar A, Kumar K, Kumar S, Chandramoni, Sinha RRK, Paswan JK, Mandal GP. 2017. Effect of feeding different level of Moringa oleifera leaf meal on growth performance, lipid profile and meat fatty acid composition of Vanaraja chicken in tropics. Indian Journal of Animal Science, 87, 5, 644-648.

    CAS  Google Scholar 

  • Kurutas EB. 2016. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutrition Journal, 15, 1, 71. doi: https://doi.org/10.1186/s12937-016-0186-5.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Lin HM, Dillard CJ, Tappel AL. 1988. Plasma SH and GSH measurement. Methods in Enzymology, 233, 380-382.

    Google Scholar 

  • Madesh M, Balasubramanian KA. 1997. A microtiter plate assay for superoxide using MTT reduction method. Indian Journal of Biochemistry and Biophysics, 34, 6, 535-9.

    CAS  PubMed  Google Scholar 

  • Makkar HPS, Becker K. 1997. Nutrients and anti-quality factors in different morphological parts of the Moringa oleifera tree. Journal of Agricultural Science Cambridge, 128, 311-332.

    Article  Google Scholar 

  • Mishra B, Jha R. 2019. Oxidative stress in the poultry gut: Potential challenges and interventions. Frontiers in Veterinary Science, 6, 60.

    Article  PubMed  PubMed Central  Google Scholar 

  • Moyo BS, Oyedemi PJ, Masika V Mucheje. 2012. Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic of liver from goats supplemented with Moringa oleifera leaves/Sunflower seed cake. Meat Science, 91, 441-447.

    Article  CAS  PubMed  Google Scholar 

  • Natt MP, Herrick CA. 1952. A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Science, 31, 4, 735-738.

    Article  Google Scholar 

  • Paul TK, Sarker YA, Tarafder M, Rimon MA, Haque MA, Sikder MH. 2018. Water supplementation of Moringa oleifera as a substitute for antibiotics on performance and blood parameters of broiler chickens broiler chickens. Journal of Bangladesh Agricultural University, 16, 2, 266–270.

  • Reyer H, Hawken R, Murani E, Ponsuksili S, Wimmers K. 2015. The genetics of feed conversion efficiency traits in a commercial broiler line. Scientific Reports, 5, 16387. doi: https://doi.org/10.1038/srep16387.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Snedecor GW, Cochran WG. 1994. Statistical Methods. 9th ed. The Iowa, State University Press, Ames, Iowa.

    Google Scholar 

  • Suleiman SA, Ali ME, Zaki ZMS, Malik EMA, Nasr MA. 1996. Lipid peroxidation and human sperm motility: protective role of vitamin E. Journal of Andrology, 17, 530-537.

    CAS  PubMed  Google Scholar 

  • Surai PF, Kochish II, Fisinin VI, Kidd MT. 2019. Antioxidant defence systems and oxidative stress in poultry biology: An update. Antioxidants, 8, 7, 235.

    Article  CAS  PubMed Central  Google Scholar 

  • Talapatra SK, Roy SC, Sen KC. 1940. The analysis of mineral constituents in biological materials. I. Estimation of phosphorus, calcium, magnesium, sodium and potassium in food stuffs. Indian Journal of Veterinary Science and Animal Husbandry, 10, 243-258.

    CAS  Google Scholar 

  • Tijani L, Akanji A, Agbalaya K, Murisiku O. 2016. Effect of Moringa leaf meal supplementation on haematological and serum biochemical profile of broiler chicken. Indian Journal of Animal Nutrition, 33, 1, 75.

    Article  CAS  Google Scholar 

  • Younis EM, Elbestawy AR. 2017. Water supplementation of Moringa oleifera and its effect on performance, blood antioxidant and immune response of two broiler breeds. Journal of Biological Sciences, 17, 52-60.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors express their gratitude to the Vice Chancellor, Bihar Animal Sciences University, Patna, India, for providing the facilities and necessary funds for conducting this experiment (communication no. BASU/PUB/02/2021).

Funding

This work was supported by Bihar Animal Sciences University, Patna, India.

Author information

Authors and Affiliations

  1. Department of Animal Nutrition, Bihar Veterinary College, Patna, 800 014, India

    Rakesh Kumar, Kaushalendra Kumar, Sanjay Kumar, Pankaj Kumar Singh & Chandra Moni

  2. Department of Biochemistry, Bihar Veterinary College, Patna, 800 014, India

    Ajeet Kumar

  3. Department of Livestock Production Management, Bihar Veterinary College, Patna, 800 014, India

    Ravi Ranjan Kumar Sinha

Authors
  1. Rakesh Kumar
    View author publications

    Search author on:PubMed Google Scholar

  2. Kaushalendra Kumar
    View author publications

    Search author on:PubMed Google Scholar

  3. Ajeet Kumar
    View author publications

    Search author on:PubMed Google Scholar

  4. Sanjay Kumar
    View author publications

    Search author on:PubMed Google Scholar

  5. Pankaj Kumar Singh
    View author publications

    Search author on:PubMed Google Scholar

  6. Ravi Ranjan Kumar Sinha
    View author publications

    Search author on:PubMed Google Scholar

  7. Chandra Moni
    View author publications

    Search author on:PubMed Google Scholar

Contributions

KK, RK and AK conceived and designed research. RK and KK conducted experiments. SK and RRKS contributed new reagents or analytical tools. PKS and CM analysed data. KK and RK wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Kaushalendra Kumar.

Ethics declarations

Ethics approval

This animal study has been approved by the Institutional Animal Ethical Committee (IAEC) of Bihar Veterinary College, Bihar Animal Sciences University, Patna, India, vide proposal no. IAEC/BVC/19/01 and performed in accordance with the ethical standard laid down.

Consent to participate

Not applicable.

Consent for publication

Not applicable.

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kumar, R., Kumar, K., Kumar, A. et al. Nutritional and physiological responses of broiler chicken to the dietary supplementation of Moringa oleifera aqueous leaf extract and ascorbic acid in tropics. Trop Anim Health Prod 53, 428 (2021). https://doi.org/10.1007/s11250-021-02864-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11250-021-02864-3

Keywords

Profiles

  1. Ajeet Kumar View author profile