Abstract
The objective was to estimate allelic and genotypic frequencies for loci associated with meat quality in a Mexican population of Braunvieh cattle. Information was obtained from 300 animals genotyped with the Genomic Profile Bovine LD chip of 30K and 50K SNPs. After the final edition, including quality control, the data contained information for 12 loci of the CAPN1, CAPN3, CAPN5, CAPN14, DGAT1, DGAT2, TG, ANK1, and MADH3 genes. Allelic and genotypic frequencies and Hardy-Weinberg equilibrium were estimated with the Cervus 3.0.7 software. The studied population markers were in Hardy-Weinberg equilibrium, except for those associated with CAPN1, DGAT1, and MADH3. Frequencies higher than those reported for other breeds were found for genotypes associated with meat softness, higher marbling score, lower quantity of saturated fatty acids, and lower shear force (CAPN1 and DGAT2). There were similarities with frequencies reported for Bos taurus breeds for the CAPN3 and TG genes. For the DGAT1 and ANK1 genes, the frequencies of the desired genotypes were low. A marker for DGAT1 and another for MADH3 were monomorphic. The results of this study are encouraging in terms of the potential of the Braunvieh population studied for breeding programs aiming to increase meat quality. The breed has strengths that could be used either by crossbreeding to generate heterozygous animals or by selection to increase frequencies of valuable alleles.
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References
Aslan, O., Sweeny, T., Mullen, A.M. and Hamill, R.M., 2010. Regulatory polymorphisms in the bovine Ankyrin 1 gene promoter are associated with tenderness and intramuscular fat content, BMC Genetics, 11, 111-1124.
Barendse, W., Harrison, B.E., Bunch, R.J. and Thomas, M.B., 2008. Variation at the Calpain 3 gene is associated with meat tenderness in zebu and composite breeds of cattle, BMC Genetics, 9(41), 1-8.
Bonilla, C.A., Rubio, M.S., Sifuentes, A.M., Parra-Bracamonte, G.M., Arellano, V.W., Méndez, M.R.D., Berruecos, J.M. and Ortiz, R., 2010. Association of CAPN1 316, CAPN1 4751 and TG5 markers with bovine meat quality traits in Mexico, Genetics and Molecular Research 9(4), 2395-2405.
Corva, P., Soria, L., Schor, A., Villarreal, E., Pérez, C.M., Motter, M., Mezzadra, C., Melucci, L., Miquel, C., Paván, E., Depetris, G., Santini, F. and Naón, J.G., 2007. Association of CAPN1 and CAST gene polymorphisms with meat tenderness in Bos taurus beef cattle from Argentina, Genetics and Molecular Biology, 30(4), 1064-1069.
Curi, R.A., Chardulo, L.A.L., Giusti, J., Silveira, A.C., Martins, C.L. and de Oliveira, H.N., 2010. Assessment of GH1, CAPN1, and CAST polymorphisms as markers of carcass and meat traits in Bos indicus and Bos taurus-Bos indicus cross beef cattle, Meat Science, 86, 915-920.
De Oliveira, S.R.M., Bonvino, S.N., de Oliveira, B.F., Ferreira, M.G.C., Matos T.C., Goncalves, J.N.S.C., Baldi, F., Boligon, A.A., Zerlotti Mercadante, M.E., Lourenco, D.L., Misztal, I. and de Albuquerque, L.G., 2017. Genome-wide association study for carcass traits in an experimental Nelore cattle population, Plos One, 12(1), e0169860.
Dunner, S., Sevane, N., García, D., Cortés, O., Valentini, A., Williams, J.L., Mangin, B., Cañón, J., Levéziel, H. and the GeMQual Consortium, 2013. Association of genes involved in carcass and meat quality traits in 15 European bovine breeds, Livestock Science, 154, 34-44.
Falconer, D.S. and Mackay, T.F.C., 1996. Introduction to Quantitative Genetics. (Pearson, Prentice Hall, New Jersey).
Fortes, M.R.S., Curi, R.A., Chardulo, L.A.L., Silveira, A.C., Assumpção, M.E.O.D., Visintin, J.A. and de Oliveira, H.N., 2009. Bovine gene polymorphisms related to fat deposition and meat tenderness, Genetics and Molecular Biology, 32(1), 75-82.
Gan, Q.F., Zhang, L.P., Li, J.Y., Hou, G.Y., Li, H.D., Gao, X., Ren, H.Y., Chen, J.B. and, Xu, S.Z., 2008. Association analysis of thyroglobulin gene variants with carcass and meat quality traits in beef cattle, Journal of Applied Genetics, 49, 251-255.
Gene., 2020. National Library of Medicine (US). National Center for Biotechnology Information (NCBI). https://www.ncbi.nlm.nih.gov/gene. Accessed 19 March, 2020.
Grisart, B., Coppieters, W., Farnir, F., Karim, L., Ford, C., Berzi, P., Cambisano N., Mni, M., Reid, S., Simon, P., Spelman, R., Georges, M. and Snell, R., 2002. Positional candidate cloning of a QTL in dairy cattle: identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition, Genome Research, 12, 222–231.
Horodyska, J., Sweeney, T., Ryan, M. and Hamill, R.M., 2015. Novel SNPs in the Ankiryn 1 gene and their association with beef quality traits, Meat Science, 108, 88-96.
Jiang, Z., Michal, J.J., Chen, J., Daniels, T.F., Kunej, T., Garcia, M.D., Gaskins, C.T., Busboom, J.R., Alexander, L.J., Wright Jr., R.W. and MacNeil, M.D., 2009. Discovery of novel genetic networks associated with 19 economically important traits in beef cattle, International Journal of Biological Sciences, 5(6), 528-542.
Kalinowski, S.T., Taper, M.L. and Marshall, T.C., 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment, Molecular Ecology, 16, 1099-1106.
Li, J., Xu, X., Zhang, Q., Wang, X., Deng, G., Fang, X., Gao, X., Ren, H. and Xu, S., 2009. Association between single nucleotide polymorphisms in the dgat2 gene and beef carcass and quality traits in commercial feedlot steers, Asian-Australasian Journal of Animal Sciences 7, 943-954.
Li, X., Ekerljung, M., Lundström, K. and Lundén, A., 2013. Association of polymorphisms at DGAT1, leptin, SCD1, CAPN1 and CAST genes with color, marbling and water holding capacity in meat from beef cattle populations in Sweden, Meat Science, 94, 153-158.
Long, A., 2001. Candidate Gene, In: Encyclopedia of Genetics, S. Brenner, and J. H. Miller, Editor(s). (Academic Press, Cambridge, MA).
Mazzucco, J.P., Melucci, L.M., Villareal, E.L., Mezzadra, C., Soria, L., Corva, P., Motter, M.M., Schor, A. and Miquel, M.C., 2010. Effect of ageing and μ-calpain markers on meat quality from Brangus steers finished on pasture, Meat Science, 86(3), 878-882.
Oliveira, B.B., Abdallah, C.R., Baldi, F., Braga, F.F.L., Fernandes, A.W.B., Galvão, A.L., Nunes, O.H. and Loyola, C.L.A., 2014. Polymorphisms in candidate genes and their association with carcass traits and meat quality in Nelore cattle, Pesquisa Agropecuária Brasileira, 49(5), 364-371.
Pannier, L., Mullen, A.M., Hamill, R.M., Stapleton, P.C. and Sweeney T., 2010. Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle, Meat Science, 85, 515-518.
Ramayo-Caldas, Y., Renand, G., Ballester, M., Saintilan, R. and Rocha D., 2016. Multi-breed and multi-trait co-association analysis of meat tenderness and other meat quality traits in three French beef cattle breeds, Genetics Selection Evolution, 48(37), 1-9.
Rubtsov, A.M. and Lopina, O.D., 2000. Ankyrins, FEBS Letters 482, 1-5.
Ruiz de H, F., Miguel, E., Onega, E. and Blázquez B., 2003. Changes in meat quality characteristics of bovine meat during the first 6 days postmortem, Meat Science, 65, 1439-1446.
Sanders, K., Bennewitz, J., Reinsch, N., Thaller, G., Prinzenberg, E.-M., Kühn, C. and Kalm, E., 2006. Characterization of the DGAT1 mutations and the CSN1S1 promoter in the German Angeln dairy cattle population, Journal of Dairy Science, 89, 3164-3174.
Souza, F.R.P., Mercadante, M.E.Z., Fonseca, L.F.S., Ferreira, M.L.S., Regatieri, I.C., Ayres, D.R., Tonhati, H., Silva, S.L., Razook, A.G. and Albuquerque, L.G., 2010. Assessment of DGAT1 and LEP gene polymorphisms in three Nelore (Bos indicus) lines selected for growth and their relationship with growth and carcass traits, Journal of Animal Science, 88, 435-441.
Tizioto, P.C., Decker, J.E., Taylor, J.F., Schnabel, R.D., Mudadu, M.A., Silva, F.L., Mourão, G.B., Coutinho, L.L., Tholon, P., Sonstegard, T.S., Rosa, A.N., Alencar, M.M., Tullio, R.R., Medeiros, S.R., Nassu, R.T., Feijó, G.L.D., Silva, L.O.C., Torres, R.A., Siqueira, F., Higa, R.H. and Regitano, L.C.A., 2013. Genome scan for meat quality traits in Nelore beef cattle, Physiological Genomics 45, 1012-1020.
Tizioto, P.C., Gromboni, C.F., de Araujo, N.A.R., de Souza, M.M., Mudadu, M.A., Tholon, P., Rosa, A.N., Tullio, R.R., Medeiros, S.R., Nassu, R.T., and Regitano, L.C.A., 2014. Calcium and potassium content in beef: Influences on tenderness and associations with molecular markers in Nelore cattle, Meat Science, 96(1), 436-440.
Yuan, Z., Li, J., Li, J., Gao, X., Gao, H. and Xu, S., 2013. Effects of DGAT1 gene on meat and carcass fatness quality in Chinese commercial cattle, Molecular Biology Reports, 40, 1947-1954.
Zhang, L.P., Gan, Q.F., Hou, G.Y., Gao, H.J., Li, J.Y. and Xu, S.Z., 2015. Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers, Genetics and Molecular Research, 14(2), 5320-5326.
Acknowledgements
The authors thank the Asociación Mexicana de Criadores de Ganado Suizo de Registro for allowing the use of their databases and the cooperation of the breeders for this study. Thanks also are extended to the Consejo Nacional de Ciencia y Tecnología, Mexico, for the financial support of the first author during her Master of Science studies.
Funding
This work was supported by a grant from the Universidad Autónoma Chapingo, Mexico (DGIP-166701012).
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MZTC wrote the first version of the manuscript, JEVH and PPR assisted MZTC during the analysis, RLO and ARF conceived the project, all the authors contributed to obtain the final version, ARF served as the correspondence author.
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Trujano-Chavez, M.Z., Valerio-Hernández, J.E., López-Ordaz, R. et al. Allelic and genotypic frequencies for loci associated with meat quality in Mexican Braunvieh cattle. Trop Anim Health Prod 53, 307 (2021). https://doi.org/10.1007/s11250-021-02757-5
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DOI: https://doi.org/10.1007/s11250-021-02757-5