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Extraction and purification of β-glucanase from bovine rumen fungus Trichoderma reesei and its effect on performance, carcass characteristics, microbial flora, plasma biochemical parameters, and immunity in a local broiler hybrid Golpayegan-Ross

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Abstract

The enzyme β-glucanase was extracted from Trichoderma reesei in bovine rumen fluid samples collected from a slaughterhouse and its effect was investigated in broilers. Data collected was broiler performance, carcass characteristics, duodenum microbial flora, hematological, and immunological parameters. β-glucanase activity was assayed through spectrometry and was approximately 0.434 IU per gram culture medium. In the current study, endoglucanase enzymes were extracted from Trichoderma reesei. A total of 160 local broilers (Golpayegan-Ross hybrid) were allocated to 4 treatments with 4 replicates per treatment. Over a 49-day experimental period, broilers were fed a basal diet (T1), basal diet plus 20% barley (T2), basal diet with 10 IU extracted β-glucanase and 20% barley (T3), and basal diet with 10 IU commercial β-glucanase and 20% barley (T4). The T3 treatment resulted in the greatest body weight gain at the end of experiment (P < 0.01). No significant differences were for feed conversion (FCR; P > 0.05). The highest cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), and LDL cholesterol ratio was observed in the T3 treatment. The highest concentrations of immunoglobulin G1 (IgG1), immunoglobulin G2 (IgG2), and immunoglobulin M1 (IgM1) were observed in the T4 treatment. The T3 treatment resulted in the best response for all measured carcass characteristics. The highest levels of aerobic bacteria, lactobacilli, anaerobic bacteria, and E. coli were associated with the T4, T3, T4, and T1 treatments, respectively. It is concluded that β-glucanase supplementation can be used to overcome the anti-nutritive effects of water soluble barley non-starch polysaccharides (NSPs) and consequently enhance broiler performance without any adverse effects on humoral immunity parameters.

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References

  • Campbell, GL, HL Classen, PA Thacker, BG Rossnagel, JWD Grootwassink, and RE Salmon. 1986. “Effect of Enzyme Supplementation on the Nutritive Value of Feedstuffs.” Proc. 7th Western Nutrition Conf. at Saskatoon, Sask, Canada 227–50.

  • Classen, HL, GL Campbell, and JWD Grootwassink. 1988. “Improved Feeding Value of Saskatchewan-Grown Barley for Broiler Chickens with Dietary Enzyme Supplementation.” Canadian Journal of Animimal Sciecnce 68:1253–59.

    Article  Google Scholar 

  • Colombatto, D., FL Mould, MK Bhat, and E. Owen. 2003. “Use of Fibrolytic Enzymes to Improve the Nutritive Value of Ruminant Diets a Bichemical and in Vitro Rumen Degradation Assessment.” Animal Feed Science and Technology (107):201–9.

    Article  CAS  Google Scholar 

  • Dalólio, FS, DP Vaz, J. Moreira, LFT Albino, and LR Valadares. 2015. “Carcass Characteristics of Broilers Fed Enzyme Complex.” Biotechnology in Animal Husbandry 31(2):153–62.

    Article  Google Scholar 

  • Dehority, BA and CG Orpin. 1997. “Development of, and Natural Fluctuations in, Rumen Microbial Populations.” The Rumen Microbial Ecosystem 196–245.

  • Elaine Turner, R. 2002. Nutrition. American Dietetic Association.730 p.

  • Ganjali, H, AR, Raji, and H. Zarghi. 2015. “Effect of post hatch delayed access to feed on performance, GIT physical and histological development and yolk absorption in young broiler chicks.” Biomedical Pharmacology Journal, 8(2):945–955.

    Article  Google Scholar 

  • Garipoglu, AV, BZ Saricicek, and U. Klic. 2006. “Effects of Commercial Enzyme Supplementation to the Rations on Broiler Performance.” Asian Journal of Animimal Veterinary Advances 1(1):23–29.

    Article  Google Scholar 

  • Ghosh, A., GP Mandal, A. Roy, and AK Patra. 2016. “Effects of Supplementation of Manganese with or without Phytase on Growth Performance, Carcass Traits, Muscle and Tibia Composition, and Immunity in Broiler Chickens.” Livestock Science 80–85.

  • Gordon, GL and MW Phillips. 2007. “The Role of Anaerobic Gut Fungi in Ruminants.” Nutrition Research Review 11(1):133–68.

    Article  Google Scholar 

  • Gryshyna, A., L. Kautto, R. Peterson, H. Nevalainen. 2016. “On the Safety of Filamentous Fungi with Special Emphasis on Trichoderma Reesei and Products Made by Recombinant Means.” Gene Expression Systems in Fungi: Advancements and Applications, Fungal Biology.

  • Hadorn, R and H Wiedmer. 2001. “Effect of an Enzyme Complex in a Wheat-Based Diet on Performance of Male and Female Broilers.” Journal Applied Poultry Research 10: 340–346

  • Hajati, H., M. Rezaie, and H. Sayyahzadeh. 2009. “The Effects of Enzyme Supplementation on Performance, Carcass Characrteristics and Some Blood Parameters of Broilers Fed on Corn-Soybean Meal-Wheat Diets.” International Journal of Poultry Science 8(12):1199–1205.

    Article  CAS  Google Scholar 

  • Jian-yi, S., L. Wei-fen, X. Zi-rong, and G. Sai-hong. 2002. “Purification and Some Properties of a β-Glucanase from a Strain Trichoderma Reesei GXC.” Of Zhejiang University Science 3(1):106–12.

    Article  Google Scholar 

  • Lakay, FM. 2001. “Fungal Enzymes as Animal Feed Additives.” University of Stellenbosch.

  • Lawal, TE, EA Lyayi, BA Adeniyi, and OA Adaramoye. 2010. “Extraction of Enzymes from Four Fungi and Their Use to Improve the Nutritive Value of Groundnut Pod for Broiler Feeding.” International Journal of Poultry Science 9(4):340–46.

    Article  CAS  Google Scholar 

  • Mathlouthi, N., N. Ballet, and M. Larbier. 2011. “Influence of Beta-Glucanase Supplementation on Growth Performances and Digestive Organs Weights of Broiler Chickens Fed Corn, Wheat and Barley-Based Diet.” International Journal of Poultry Science 10(2):157–59.

    Article  CAS  Google Scholar 

  • Miettinen-Oinonen, A. 2004. “Trichoderma Reesei Strains for Production of Cellulases for the Textile Industry.” Epsoo 2004. VVT Publications 550 53p.

  • Morgan, A, M Bedford, A Tervila-Wilo, M Hopeakoski-Nurminen, K Autio, K Poutanen, and T. Parkkonen. 1995. “How enzymes improve the nutritional value of wheat.” Zootechnica International. 4:44–48.

  • Munyaka PM, Nandha NK, Kiarie E, Nyachoti CM, and Khafipour E. 2015. “Impact of Combined β-Glucanase and Xylanase Enzymes on Growth Performance, Nutrients Utiliztion and Gut Microbiota in Broiler Chickens Fed Corn or Wheat-Based Diets.” Poultry Sci 95:528–540.

  • Ozek, K, O Yazgan, and Y. Bahtiyarca. 2003. Effects of dietary protein and energy concentrations on performance and carcass characteristics of chukar partridge (Alectoris chukar) raised in captivity.” British Poultry Science 44:419–426.

    Article  CAS  Google Scholar 

  • Paloheimo, M., T. Haarmann, S. Mäkinen, and J. Vehmaanperä. 2016. “Production of Industrial Enzymes in Trichoderma Reesei.” Gene Expression Systems in Fungi: Advancements and Applications, Fungal Biology.

  • Poorghasemi, M., M. Chamani, S.Z. Mirhosseini, A.A. Sadeghi, and A. Seidavi. 2017. “Effect of probiotic and different sources of fat on performance, carcass characteristics, intestinal morphology and ghrelin gene expression on broiler chickens.” Kafkas Universitesi Veteriner Fakultesi Dergisi 24(2):169–178.

    Google Scholar 

  • Rosana, Yeva, Tetsuhiro Matsuzawa, Tohru Gonoi, and Anis Karuniawati. 2014. “Modified Slide Culture Method for Faster and Easier Identification of Dermatophytes.” 8(3):135–39.

  • Saloheimo, M. and TM Pakula. 2012. “The Cargo and Transport System: Secreted Proteins and Protein Secretion in Trichoderma Reesei (Hypocera Jecorina).” Microbiology (158):46–57.

    Article  CAS  Google Scholar 

  • Sarangi, NR, LK Babu, A. Kumar, CR Pradhan, PK Pati, and P. Mishra. 2016. “Effect of Dietary Supplementation of Prebiotic, Probiotic, and Synbiotic on Growth Performance and Carcass Characteristics of Broiler Chickens.” Veterinary World.

  • Trinci, APJ, DR Davies, K. Gull, MI Lawrence, BB Nielsen, A. Rickers, and MK Theodorou. 1994. “Aaerobic Fungi in Herbivorous Animals.” Mycolology Research 98(2):129–52.

    Article  Google Scholar 

  • Wilson, DB. 2011. “Microbial Diversity of Cellulose Hydrolysis.” Current Opinion Microbiology 14:1–5.

    Article  Google Scholar 

  • Yu, B., JC Hsu, and PWS Chiou. 1998. “Effects of β-Glucanase Supplementation of Barley Diets on Growth Performance of Broilers.” Animal Feed Science Technology (70):353–61.

    Article  CAS  Google Scholar 

  • Zhang, Z., J. Liu, J. Lan, C. Duan, Q. Ma, and J. Feng. 2014. “Predominance of Trichoderma and Penicillium in Cellulolytic Aerobic Filamentous Fungi from Subtropical and Tropical Forests in China, and Their Use in Finding Highly Efficient β-Glucosidase.” Biotechnology for Biofuels 7:107.

    Article  Google Scholar 

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Acknowledgments

This manuscript is prepared based on PhD thesis of first author at Varamin-Pishva Branch, Islamic Azad University, Tehran, Iran. We are grateful to the Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran.

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Authors and Affiliations

  1. Department of Animal Science, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran

    Mohammadebrahim Tasirnafas, Kazem Karimi & Ghobad Asgari Jafarabadi

  2. Department of Animal Science, Rasht Branch, Islamic Azad University, Rasht, Iran

    Alireza Seidavi

  3. Department of Microbiology, Biological Science College, Varamin-Pishva Branch, Islamic Azad University, Varamin-Pishva, Iran

    Fatemeh Noorbakhsh

Authors
  1. Mohammadebrahim Tasirnafas
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  2. Kazem Karimi
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  3. Ghobad Asgari Jafarabadi
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  4. Alireza Seidavi
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  5. Fatemeh Noorbakhsh
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Correspondence to Kazem Karimi.

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The use of chicks in this experiment was approved by the local institutional animal ethical committee.

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Tasirnafas, M., Karimi, K., Asgari Jafarabadi, G. et al. Extraction and purification of β-glucanase from bovine rumen fungus Trichoderma reesei and its effect on performance, carcass characteristics, microbial flora, plasma biochemical parameters, and immunity in a local broiler hybrid Golpayegan-Ross. Trop Anim Health Prod 52, 1833–1843 (2020). https://doi.org/10.1007/s11250-019-02186-5

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