Evaluating the effectiveness of two bovine mastitis vaccines and their influences on oxidant and antioxidant capacities of milk

Abstract

The aim of the current study was to investigate the efficacy of two commercial mastitis vaccines (Startvac® and Mastivac®) and their influence on oxidant and antioxidant capacities of milk samples in a dairy farm. A total of 165 Holstein dairy cows were recruited into the study and were divided into Startvac®, Mastivac®, and control groups. The effects on the incidence and severity of clinical mastitis cases, duration of treatment, infection status, bacteriologic culture, somatic cell count, 5-thio-2-nitrobenzoic acid assay and ferric reducing antioxidant power assay, incidence of metritis and endometritis, and milk yield were evaluated within the first 90 days of lactation. The incidence of clinical mastitis was not significantly different among groups. The mean SCCs during the first, second, and third months of lactation did not differ significantly. The percentage of cured cows did not differ significantly. TNB and FRAP assays were used to know whether or not vaccination against mastitis has an effect on oxidant and antioxidant capacity of milk samples, which did not differ significantly. In conclusion, we observed no significant difference in the abovementioned variables. However, it is possible that applying mastitis vaccines within the specific conditions may have positive effects considering the results of previous studies.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Abbreviations

TNB:

Thio-nitrobenzoate

FRAP

Ferric reducing antioxidant power

SCC

Somatic cell count

TOC

Total oxidant capacity

TAC

Total antioxidant capacity

S. aureus

Staphylococcus aureus

E. coliEscherichia coli

CNS

Coagulase-negative staphylococci

References

  1. Atakisi, O., Oral, H., Atakisi, E., Merhan, O., Pancarci, S. M., Ozcan, A., Marasli, S., Polat, B., Colak, A. & Kaya, S. 2010. Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Research in Veterinary Science, 89, 10-13.

    CAS  Article  Google Scholar 

  2. Barlund, C., Carruthers, T., Waldner, C. & PALMER, C. 2008. A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology, 69, 714-723.

    CAS  Article  Google Scholar 

  3. Bradley, A. J., Breen, J., Payne, B., White, V. & Green, M. J. 2015. An investigation of the efficacy of a polyvalent mastitis vaccine using different vaccination regimens under field conditions in the United Kingdom. Journal of Dairy Science, 98, 1706-1720.

    CAS  Article  Google Scholar 

  4. Council, N. M. 1999. Laboratory handbook on bovine mastitis. NMC Inc., Madison.

    Google Scholar 

  5. Erskine, R. 2012. Vaccination strategies for mastitis. Veterinary Clinics: Food Animal Practice, 28, 257-270.

    CAS  Google Scholar 

  6. Freick, M., Frank, Y., Steinert, K., Hamedy, A., Passarge, O. & Sobiraj, A. 2016a. Mastitis vaccination using a commercial polyvalent vaccine or a herd-specific Staphylococcus aureus vaccine. Tierärztliche Praxis Ausgabe G: Großtiere/Nutztiere, 44, 219-229.

    Article  Google Scholar 

  7. Freick, M., Frank, Y., Steinert, K., Hamedy, A., Passarge, O. & Sobiraj, A. 2016b. Mastitis vaccination using a commercial polyvalent vaccine or a herd-specific Staphylococcus aureus vaccine. Tierärztliche Praxis G: Großtiere/Nutztiere, 44, 219-229.

    Google Scholar 

  8. Hernández-Castellano, L. E., Wall, S., Stephan, R., Corti, S. & Bruckmaier, R. 2017. Milk somatic cell count, lactate dehydrogenase activity, and immunoglobulin G concentration associated with mastitis caused by different pathogens: A field study. Schweiz. Arch. Tierheilkd, 159, 283-290.

    Article  Google Scholar 

  9. Kusebauch, U., Hernández-Castellano, L. E., Bislev, S. L., Moritz, R. L., Røntved, C. M. & Bendixen, E. 2018. Selected reaction monitoring mass spectrometry of mastitis milk reveals pathogen-specific regulation of bovine host response proteins. Journal of Dairy Science, 101, 6532-6541.

    CAS  Article  Google Scholar 

  10. Landin, H., Mörk, M. J., Larsson, M. & Waller, K. P. 2015. Vaccination against Staphylococcus aureus mastitis in two Swedish dairy herds. Acta Veterinaria Scandinavica, 57, 81.

    Article  Google Scholar 

  11. Leitner, G., Lubashevsky, E., Glickman, A., Winkler, M., Saran, A. & Trainin, Z. 2003. Development of a Staphylococcus aureus vaccine against mastitis in dairy cows: I Challenge trials. Veterinary Immunology and Immunopathology, 93, 31-38.

    CAS  Article  Google Scholar 

  12. Piepers, S., Prenafeta, A., Verbeke, J., DE Visscher, A., March, R. & DE Vliegher, S. 2017. Immune response after an experimental intramammary challenge with killed Staphylococcus aureus in cows and heifers vaccinated and not vaccinated with Startvac, a polyvalent mastitis vaccine. Journal of Dairy Science, 100, 769-782.

    CAS  Article  Google Scholar 

  13. Ruegg, P. 2001. Evaluating the effectiveness of mastitis vaccines. UW Milk Quality.

  14. Schukken, Y., Bronzo, V., Locatelli, C., Pollera, C., Rota, N., Casula, A., Testa, F., Scaccabarozzi, L., March, R. & Zalduendo, D. 2014. Efficacy of vaccination on Staphylococcus aureus and coagulase-negative staphylococci intramammary infection dynamics in 2 dairy herds. Journal of Dairy Science, 97, 5250-5264.

    CAS  Article  Google Scholar 

  15. Silanikove, N., Merin, U., Shapiro, F. & Leitner, G. 2014. Subclinical mastitis in goats is associated with upregulation of nitric oxide-derived oxidative stress that causes reduction of milk antioxidative properties and impairment of its quality. Journal of dairy science, 97, 3449-3455.

    CAS  Article  Google Scholar 

  16. Talbot, B. G. & Lacasse, P. 2005. Progress in the development of mastitis vaccines. Livestock production science, 98, 101-113.

    Article  Google Scholar 

  17. Wall, S. K., Hernández-Castellano, L. E., Ahmadpour, A., Bruckmaier, R. M. & Wellnitz, O. 2016. Differential glucocorticoid-induced closure of the blood-milk barrier during lipopolysaccharide-and lipoteichoic acid-induced mastitis in dairy cows. Journal of Dairy Science, 99, 7544-7553.

    CAS  Article  Google Scholar 

  18. Wilson, D. J., Grohn, Y., Bennett, G., González, R., Schukken, Y. & Spatz, J. 2007. Comparison of J5 vaccinates and controls for incidence, etiologic agent, clinical severity, and survival in the herd following naturally occurring cases of clinical mastitis. Journal of Dairy Science, 90, 4282-4288.

    CAS  Article  Google Scholar 

Download references

Acknowledgments

The authors would like to gratefully acknowledge Mr. A. Kargar for his assistance in this project; also, Startvac® and Mastivac® vaccines were kind gifts of Sorur Fajr Co. and Nikan Pakhsh Behparvar companies (Tehran, Iran), respectively.

Funding

This work was supported by the Ferdowsi University of Mashhad (Grant number 3/39422, December/20/2015). Funding was allocated to collection, analysis and interpretation of data.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Babak Khoramian.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Tashakkori, N., Khoramian, B., Farhoodi Moghadam, M. et al. Evaluating the effectiveness of two bovine mastitis vaccines and their influences on oxidant and antioxidant capacities of milk. Trop Anim Health Prod 52, 1493–1501 (2020). https://doi.org/10.1007/s11250-019-02156-x

Download citation

Keywords

  • Mastitis
  • Vaccine
  • Dairy cow
  • Oxidant and antioxidant capacities