Abstract
The present study aimed to evaluate the degradation dynamics of high-quality tropical forage according to the levels of nitrogen supplementation. In this study, 4 rumen-fistulated cows with a body weight of 653 ± 47 kg were used. These animals were distributed in a 4 × 4 Latin square experimental design. Cows were kept under a grazing regime in an area formed by Panicum maximum Jacq. cv. Tanzania. These animals received 4 levels of crude protein (CP) in the supplements (122, 142, 162, and 180 g/kg of CP in DM). The degradability of dry matter (DM), crude protein (CP), and neutral detergent fiber (NDF) was determined by in situ incubation of forage samples at 0, 6, 12, 24, 48, 72, 96, and 120 h. Both the concentration of ammonia nitrogen (NH3-N) and the microbial protein synthesis were affected by CP levels of the supplements (P < 0.05). There was a significant difference (P < 0.05) for fraction b, potential degradability, and effective degradability of DM and NDF, and for the c degradation rate of CP of Tanzania grass which had the highest values at 122 g/kg of CP. Overall, the greatest degradation of nutrients from Tanzania grass occurred in the diet with 122 g/kg of CP and 604 g/kg of NFC in dairy cattle supplements that resulted in the highest rates of nitrogen utilization by rumen microbes.
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References
Agricultural Research Council (ARC), 1984. The nutrient requirements of ruminant livestock, Supplement No. 1, (Commonwealth Agricultural Bureaux, London).
Carvalho, I. P. C. D., Detmann, E., Mantovani, H. C., Paulino, M. F., Valadares Filho, S. D. C., Costa, V. A. C. and Gomes, D. I., 2011. Growth and antimicrobial activity of lactic acid bacteria from rumen fluid according to energy or nitrogen source. Revista Brasileira de Zootecnia, 40 (6), 1260–1265.
Casali, A. O., Detmann, E., Valadares Filho, S. C., Pereira, J. C., Henriques, L. T., Freitas, S. G. and Paulino, M. F., 2008. Influência do tempo de incubação e do tamanho de partículas sobre os teores de compostos indigestíveis em alimentos e fezes bovinas obtidos por procedimento in situ. Revista Brasileira de Zootecnia, 37 (2), 335–342.
Chaney, A. L. and Marbach, E. P., 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry, 8,130–132.
Chen, X. B. and Gomes, M. J., 1992. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives - An overview of technical details. International Feed Resources Unit, Rowett Research Institute, Aberdeen, UK.
Chizzotti, M. L., Valadares Filho, S. C., Valadares, R. F. D., Chizzotti, F. H. M. and Tedeschi, L. O., 2008. Determination of creatinine excretion and evaluation of spot urine sampling in Holstein cattle. Livestock Science, 113, 218–225.
Costa, V. A. C., Detmann, E., Valadares Filho, S. C., Paulino, M. F., Henriques, L. T. and Mantovani, H. C., 2009. Degradação in vitro da fibra em detergente neutro de forragem tropical de alta qualidade em função da suplementação com proteína e/ou carboidratos. Revista Brasileira de Zootecnia, 38(9), 1803–1811.
Costa, N. D. L., Monteiro, A. L. G., Silva, A. L. P., Moraes, A. D., Giostri, A. F., Stivari, T. S. S., Gilaverte, S., Baldissera, T. C. and Pina, E. A., 2015. Considerations on the fiber degradation of tropical forage associated with energy or nitrogenous supplements. Archivos de zootecnia, 64, 31–41.
Danes, M. A. C., Chagas, L. J., Pedroso, A. M. and Santos, F. A. P., 2013. Effect of protein supplementation on milk production and metabolism of dairy cows grazing tropical grass. Journal of Dairy Science, 96, 407–419.
Delevatti, L. M., Cardoso, A. S., Barbero, R. P., Leite, R. G., Romanzini, E. P., Ruggieri, A. C. and Reis, R. A., 2019. Effect of nitrogen application rate on yield, forage quality, and animal performance in a tropical pasture. Scientific reports, 9 (1), 7596.
Detmann, E., Souza, M.A., Valadares Filho, S.C., Queiroz, A.C., Berchielli, T.T., Saliba, E.O.S., Cabral, L. S., Pina, D.S., Ladeira, M. M. and Azevedo, J. A. G., 2012. Métodos para análises de alimentos, Instituto Nacional de Ciencia e Tecnologia – INCT (Suprema: Visconde do Rio Branco, Brazil). ISBN: 9788581790206.
Jin, D., Zhao, S., Zheng, N., Beckers, Y., and Wang, J., 2018. Urea metabolism and regulation by rumen bacterial urease in ruminants - a review. Annals of Animal Science, 18 (2), 303–318.
Licitra, G., Hernandez, T. M. and Van Soest, P. J., 1996. Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57, 347–358.
McNamara, J. P., Auldist, M. J., Marett, L. C., Moate, P. J and Wales, W. J., 2017. Analysis of pasture supplementation strategies by means of a mechanistic model of ruminal digestion and metabolism in the dairy cow. Journal of Dairy Science, 100 (2), 1095–1106.
National Research Council (NRC) 2001. Nutrient Requirements of Dairy Cattle, (National Academy Press, Washington).
Ørskov, E. R. and McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation weighted according to rate of passage. Journal of Agriculture Science, 92, 499–503.
Reed, K. F., Bonfa, H. C., Dijkstra, J., Casper, D. P. and Kebreab, E. 2017. Estimating the energetic cost of feeding excess dietary nitrogen to dairy cows. Journal of Dairy Science, 100 (9), 7116–7126.
Silva, J. A., Cabral, L. S., Costa, R. V., Macedo, B. G., Bianchi, I. E., Teobaldo, R. W., Neves, C. G., Carvalho, A. P. S., Plothow, A. F., Júnior, W. S. C. and Silva, C. G. M., 2015. Estratégias de suplementação de vacas de leite mantidas em pastagem de gramínea tropical durante o período das águas. PubVet, 9 (3), 150–157.
Sniffen, C. J., O'Connor, J. D., Van Soest, P. J., Fox, D. G. and Russell, J. B., 1992. A net carbohydrate and protein system for evaluating cattle diets. 2. Carbohydrate and protein availability. Journal Animal Science, 70, 3562–3577.
Uddin, M. J., Khandaker, Z. H., Khan, M. and Khan, M. M. H., 2015. Dynamics of microbial protein synthesis in the rumen - A review. Annals of Veterinary and Animal Science, 2 (5), 116–131.
Valadares, R. F. D., Broderick, G. A., Valadares Filho, S. C. and Clayton, M. K., 1999. Effect of replacing alfafa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives. Journal of Dairy Science, 82 (12), 2686–2696.
Acknowledgments
We are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Agência Paulista de Tecnologia dos Agronegócios (APTA) for supporting this study.
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All animal care and handling procedures were approved by the Animal Care and Use Committee of the Universidade Estadual Paulista “Júlio de Mesquita Filho,” Brazil (protocol CEUA-UNESP 026103/13).
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Pinheiro, J.K., Ferreira, R.R., Gonzaga Neto, S. et al. Dynamics of degradation of high-quality tropical forage according to levels of nitrogen supplementation. Trop Anim Health Prod 52, 1309–1314 (2020). https://doi.org/10.1007/s11250-019-02136-1
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DOI: https://doi.org/10.1007/s11250-019-02136-1