Genetic polymorphisms in part of intron 7 and exon 8 of HSP90AA1 gene and its association with heat tolerance traits in two exotic layer chicken strains

Abstract

Chickens, like other vertebrates, react to stress conditions through their cultured cells by expressing heat shock proteins (HSPs). Genetic association of single-nucleotide polymorphisms (SNPs) in HSPs with desirable traits will reveal their importance as potential genetic markers. Blood samples were collected from 50 birds per strain for DNA extraction, polymerase chain reaction amplification and sequencing of the HSP90AA1 gene. SNPs were detected using Codoncode Aligner. Association of each SNP with heat tolerance traits was analysed using generalized linear model procedure of SAS. A total of seven SNPs were detected, four SNPs; A7T, A160T, T223A and C134T were detected in part of intron 7 to exon 8 of HSP90AA1 gene of BRD while three A160T, T223A and C134T were detected in HYL. SNPC134T, a synonymous variant, was detected in exon 8. Only SNPA7T was in Hardy-Weinberg equilibrium (X2 = 0.03) but had no association with the traits measured. Polymorphic information content calculated showed SNPA160T to be moderately polymorphic; other SNPs were lowly polymorphic. Heterozygosity for SNPs-A160T and T223A of BRD showed moderate genetic variation while the other SNPs and those in HYL recorded low genetic variation. The study concluded that the SNPs detected were majorly lowly polymorphic and also the SNP locus A7T in intron 7 of HSP90AA1 of BRD had no genetic association with heat tolerance traits.

This is a preview of subscription content, access via your institution.

Fig 1
Plate 1
Plate. 2

References

  1. Ayo, J.O. Obidi, J.A. and Rekwot, P.I. 2011. Effects of Heat Stress on theWell-Being, Fertility, and Hatchability of Chickens in the Northern Guinea Savannah Zone of Nigeria: A Review. International Scholarly Research Network ISRN Veterinary Science Volume 2011, Article ID 838606, 10 pages doi:10.5402/2011/838606.

  2. Botstein D., White R.L., Skolnick M.H., Davies R.W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphism. America Journal of Human Genetics. 32: 314–331.

    CAS  Google Scholar 

  3. Craig, E. 1985. The heat shock response. Critical Review of Biochemistry. 18:239–280.

    CAS  Article  Google Scholar 

  4. Dominant CZ. 2014. Parent Stock; Common Management Guide, Layers Programmes. Lazne Bohdanec. Czech Republic. 119: 533 41. www.dominant-cz.cz. Accessed 12 June 2015.

  5. EL-Gendy, E. and Washburn, K.W. 1995. Genetic variation in body temperature and its response to short-term acute heat stress in broilers. Poultry Science. 74:225–230.

    CAS  Article  Google Scholar 

  6. FAOSTAT. 2007. Food and Agricultural Organization statistical databases. CDROM.

  7. Finch, V.A., 1986. Body temperature in beef cattle, its control and relevance to production in Tropics. Journal of Animal Science. 62: 531–538

    Article  Google Scholar 

  8. GuhaThakurta, D., Xie, T., Anand, M., Edwards, S., Li, G., Wang, S. and Schadt, E. 2006. Cis-regulatory variations: a study of SNPs around genes showing cis-linkage in segregating mouse populations. BMC Genomics. 7(1):235.

    Article  Google Scholar 

  9. Gupta R.S. 1995. Phylogenetic analysis of the 90 kD heat shock family of protein sequences, an examination of the relationship among animals, plants, fungi species. Mol. Biol. Evol. 12(6):1062–1073. https://doi.org/10.1093/oxfordjournals.molbev.a040281

    Article  Google Scholar 

  10. Hagmann, M. 1999. A good SNP may be hard to find. Science. 285(5424): 21–22.

    CAS  Article  Google Scholar 

  11. Hall, T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nuclei Acids Symposium. 41: 95–98.

    CAS  Google Scholar 

  12. Hyline. 2014. Hyline Brown: Management guide, Commercial Layers. Hyline International. www.hyline.com

    Google Scholar 

  13. Kalmar, B. and Greensmith, L. 2009. Induction of heat shock proteins for protection against oxidative stress. Advanced Drug Delivery Review. 61, 310–318.

    CAS  Article  Google Scholar 

  14. Kumar, R., Gupta I. D, Verma, A, Verma, N, Vineeth, M.R. 2015. Genetic polymorphisms within exon 3 of heat shock protein 90AA1 gene and its association with heat tolerance traits in Sahiwal cows. Veterinary World 8(7):932–936.

    CAS  Article  Google Scholar 

  15. Kumar, R., Gupta, I. D., Verma, A., Verma, N. and Vineeth, M. R. 2017. Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows. Indian Journal of Animal Research. 51 (1) 64–69. https://doi.org/10.18805/ijar.7081.

    Article  Google Scholar 

  16. Leandro, N.S.M., Gonzales, E., Ferro, J.A., Ferro, M.I.T., Givisiez, P.E.N., and Macari, M., 2004. Expression of heat shock protein in broiler embryo tissues after acute cold or heat stress. Molecular Reproduction and Development. 67:172–177.

    CAS  Article  Google Scholar 

  17. Liu, Y., Li, D., Li, H., Zhou, X. and Wang, G. 2011. A novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows. Molecular Biology Reports. 38:83–88.

    CAS  Article  Google Scholar 

  18. Marcos-Carcavilla, A., Mutikainen, M., González, C., Jorge, H., Calvo, J., Kantanen, A., Nurbiy, S., Marzanov-María, D. and Magdalena Serrano, J.B. 2010. A SNP in the HSP90AA1 gene 5′ flanking region is associated with the adaptation to differential thermal conditions in the ovine species. Cell Stress Chaperones. 15: 67–81.

    CAS  Article  Google Scholar 

  19. Maurano, M.T., Humbert, R., Rynes, E., Thurman, R.E., Haugen, E., Wang, H., Reynolds, A.P., Sandstrom, R., Qu, H., Brody, J. 2012. Systematic localization of common disease-associated variation in regulatory DNA. Science. 337: 1190–1195.

    CAS  Article  Google Scholar 

  20. Millar, D.S., Horan, M., Chuzhanova, N.A. and Cooper, D.N. 2010. Characterization of a functional intronic polymorphism in the human growth hormone (GH1) gene. Human Genomics. 4: 289–301.

    CAS  Article  Google Scholar 

  21. Pearl, L.H. and Prodromou, C. 2006. Structure and mechanism of the HSP90 molecular chaperone machinery. In Annual Review of Biochemistry; Annual Reviews: Palo Alto. 75: 271–294.

  22. Pescatello, L.S. and Roth S.M. (eds.). 2011. Exercise Genomics, Molecular and Translational Medicine, Humana Press. 287. ISBN: 9781607613541.

  23. Reddacliff, L.A., Beh, K., Mc Gregor, H. and Whittington, R.J. 2005. A preliminary study of possible genetic influences on the susceptibility of sheep to Johne’s disease. Australian Veterinary Journal. 83:435–441.

    CAS  Article  Google Scholar 

  24. Sailo, L. and Gupta, L.D., Archana Verma, Ramendra Das and M. V. Chaudhar, 2015. Association of single nucleotide polymorphism of HSP90ab1 gene with thermotolerance and milk yield in Sahiwal cows. African Journal of Biochemistry Research. 9(8): 99–103, https://doi.org/10.5897/AJBR2015.0837

  25. SAS, 2009. Statistical Analysis System User’s Guide. Statistical Analysis Institute, Inc., Cary, North Carolina.

    Google Scholar 

  26. Sauna, Z.E., Kimchi-Sarfaty, C., Ambudkar, S.V. and Gottesman, M.M. 2007. Silent polymorphisms speak: how they affect pharmacogenomics and the treatment of cancer. Cancer Research. 67, 9609–9612.

    CAS  Article  Google Scholar 

  27. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research. 25, 4876–4882.

    CAS  Article  Google Scholar 

  28. Timperio, A.M.; Eqidi, M.G.; Zolla, L. 2008. Proteomics applied on plant abiotic stresses: Role of heat shock proteins (HSP). Journal of Proteomics. 71:391–411.

    CAS  Article  Google Scholar 

  29. Yalçin, S., Ozkan, S., Cabuk, M., Buyse, J., Ducuypere, E., and Siegel, P.B. 2005. Pre- and post-natal conditioning responses and relative asymmetry of broilers originating from young and old breeder flocks. Poultry Science. 84: 967–976.

    Article  Google Scholar 

Download references

Funding

The World Bank Africa Centre of Excellence in Agricultural Development and Sustainable Environment anchored in the Federal University of Agriculture, Abeokuta. Ogun State, Nigeria, sponsored this project (Sponsor ID No: ACE 023).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Young I. Irivboje.

Ethics declarations

Ethical approval

The experiment was conducted following the code of ethics for animal experimentation with prior approval by the University’s Animal Ethics Committee.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The author Prof. Olowofeso Olajide passed away on the 29th January 2019.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Irivboje, Y.I., Sanni, M.T., Fafiolu, A.O. et al. Genetic polymorphisms in part of intron 7 and exon 8 of HSP90AA1 gene and its association with heat tolerance traits in two exotic layer chicken strains. Trop Anim Health Prod 52, 969–977 (2020). https://doi.org/10.1007/s11250-019-02079-7

Download citation

Keywords

  • HSP90AA1
  • Single-nucleotide polymorphism (SNP)
  • Exon
  • Intron
  • Heat tolerance traits
  • Layers