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Genetic survey of cystic echinococcosis in farm animals in Oman

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Abstract

Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis (CE) in animals and humans with a worldwide distribution affecting pastoral and agro-pastoral communities. Little is known about the genetic diversity and public health significance of E. granulosus s.l. among animals and human in Oman. This study was undertaken to investigate the circulating genotypes of E. granulosus s.l. in farm animals (camels, cattle, goats and sheep) by using multiplex PCR (mPCR) and sequence analysis of a fragment of the NADH dehydrogenase subunit 1 (NADH-1) gene. In this study, 39 hydatid cyst isolates from dromedary camels (n = 17), cattle (n = 12), goats (n = 9) and sheep (n = 1) from five governorates in Oman were collected. These isolates were analysed genetically to classify E. granulosus s.l. using a single-tube mPCR and further subjected to sequence analysis of mitochondrial NADH-1 gene. The results of mPCR revealed that most of the cyst isolates (71.8%) belonged to E. granulosus sensu stricto G1/G2/G3 genotypes, whereas 28.2% were linked to E. canadensis G6/G7 genotypes. However, sequencing of these isolates has confirmed the existence of the two genotypes E. canadensis G6 and E. granulosus sensu stricto G1 genotype. This study provides a molecular evidence of E. granulosus s.l. genotypes in Oman and confirms the predominance of the sheep and camel strains and their role in the transmission dynamics of CE in animals. The study will serve as a foundation for future planning and implementation for CE control program in Oman.

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Acknowledgements

We are thankful to Mr. Mahir AlMaawali, Director of Animal Health Research Centre, for his support during this research. The authors are very grateful to the Ministry of Municipality for the cooperation and support during this study.

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The Agriculture & Fisheries Development Fund, Ministry of Agriculture and Fisheries, Oman, funded this research work.

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  1. Animal Health Research Centre, Ministry of Agriculture and Fisheries, Muscat, Oman

    Fadya Abdullah AlKitani, Shumoos AlRiyami & Muhammad Hammad Hussain

  2. Departement of Biology, College of Science, Sultan Qaboos University, Muscat, Oman

    Senan Baqir & Derek Roberts

  3. Departement of Microbiology, Cholistan University of Veterinary and Animal Science, Bahawalpur, Pakistan

    Muhammad Khalid Mansoor

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  1. Fadya Abdullah AlKitani
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AlKitani, F.A., Baqir, S., Mansoor, M.K. et al. Genetic survey of cystic echinococcosis in farm animals in Oman. Trop Anim Health Prod 52, 331–337 (2020). https://doi.org/10.1007/s11250-019-02019-5

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