In this study, 600 1-day-old Japanese quail chicks (Coturnix coturnix japonica) were used to investigate the effects of bacteriocin and organic acids on performance and intestinal histomorphology and microbiology. Chicks were allocated to 6 groups, i.e., control, Bac150 (150 mg/kg bacteriocin), Bac300 (300 mg/kg bacteriocin), OA (3 g/kg organic acid blend), Bac150+OA (150 mg/kg bacteriocin + 3 g/kg organic acid blend), and Bac300+OA (300 mg/kg bacteriocin + 3 g/kg organic acid blend) group. The trial lasted 35 days. At the end of the trial, a statistical increase was not observed in the performance parameters of chicks with feed additives. However, 300 mg/kg bacteriocin + 3 g/kg organic acid supplementation given together has been found to have more positive effects on intestinal microbiology and histomorphology (P < 0.05). Consequently, it is understood that the use of these feed additives together will achieve better results.
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Açıkgöz, Z,, Bayraktar, H,, Altan, Ö, 2011. Effects of formic acid administration in the drinking water on performance, intestinal microflora and carcass contamination in male broilers under high ambient temperature, Asian-Australasian Journal of Animal Sciences, 24(1), 96–102
AOAC, 1997. Official Methods of Analysis. Association of Analytical Chemists, 16th ed. Washington D.C.
Bonos, E.M., Christaki, E.V., Florou-Paneri, P.C., 2010. Performance and carcass characteristics of Japanese quail as affected by sex or mannan oligosaccharides and calcium propionate, South African Journal of Animal Science, 40(3), 173–184
Cao, G.T., Zeng, X.F., Chen, A.G., Zhou, L., Zhang, L., Xiao, Y.P., Yang, C.M., 2013. Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response and cecal microflora in broiler chickens challenged with Escherichia coli K88, Poultry Science, 92(11), 2949–2955
Chen, C.Y., Yu, C., Chen, S.W., Chen, B.J., Wang, H.T., 2013. Effect of yeast with bacteriocin from rumen bacteria on growth performance, caecal flora, caecal fermentation and immunity function of broiler chicks, Journal of Agricultural Science, 151(2), 287–297
Chen, C.Y., Chen, S.W., Wang, H.T., 2017. Effect of supplementation of yeast with bacteriocin and Lactobacillus culture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens, Asian-Australasian Journal of Animal Sciences, 30(2), 211–220
Fazilat, H., Kheiri, F., Faghani, M., 2014. Effects of using commercial GLOBACID® acidifier supplementation on growth performance and some haematological parameters in Japanese quail (Coturnix japonica), Research Opinions in Animal & Veterinary Sciences, 4(11), 622–625
FEFANA, 2014. Organic Acids in Animal Nutrition. Fefana Publication, Brussel, Belgium. ISBN 978-2-9601289-2-5
Gabriel, I., Lessire, M., Mallet, S., Guillot, J.F., 2006. Microflora of the digestive tract: Critical factors and consequences for poultry, World’s Poultry Science Journal, 62, 499–511
Ghasemi, H.A., Salamat, H.A., Hajkhodadadi, I., Farahani, A.H.K., 2014. Effects of dietary organic acid blend supplementation on performance, intestinal morphology and antibody-mediated immunity in broiler chickens, Acta Advances in Agricultural Sciences, 2(10), 64–74
Ghazalah, A.A., Atta, A.M., Kout, E., Moustafa, M. EL., Shata, R.F.H., 2011. Effect of dietary supplementation of organic acids on performance, nutrients digestibility and health of broiler chicks, International Journal of Poultry Science, 10(3), 176–184
Giannenas, I., Papaneophytou, C.P., Tsalie, E., Pappas, I., Triantafillou, E., Tontis, D., Kontopidis, G.A., 2014. Dietary supplementation of benzoic acid and essential oil compounds affects buffering capacity of the feeds, performance of turkey poults and their antioxidant status, pH in the digestive tract, ıntestinal microbiota and morphology, Asian-Australasian Journal of Animal Sciences, 27(2) 225–236
Islam, M.Z., Khandaker, Z.H., Chowdhury, S.D., Islam, K.M.S., 2008. Effect of citric acid and acetic acid on the performance of broilers, Journal of the Bangladesh Agricultural University, 6(2), 315–320
Józefiak, D., Sip, A., Rawski, M., Steiner, T., Rutkowski, A., 2011. The dose response effects of liquid and lyophilized Carnobacterium divergens AS7 bacteriocin on the nutrient retention and performance of broiler chickens, Journal of Animal and Feed Sciences, 20(1), 401–411
Józefiak, D., Sip, A., Rutkowski, A., Rawski, M., Kaczmarek, S., Wołuń-Cholewa, M., Engberg, R.M., Højberg, O., 2012. Lyophilized Carnobacterium divergens AS7 bacteriocin preparation improves performance of broiler chickens challenged with Clostridium perfringens, Poultry Science, 91(8), 1899–1907
Józefiak, D., Kierończyk, B., Juśkiewicz, J., Zduńczyk, Z., Rawski, M., Długosz, J., Sip, A., Højberg, O., 2013. Dietary nisin modulates the gastrointestinal microbial ecology and enhances growth performance of the broiler chickens, Plos One, 8(12), 1–11
Kierończyk, B., Pruszyńska-Oszmałek, E., Świątkiewicz, S., Rawski, M., Długosz, J., Engberg, R.M., Józefiak, D., 2016. The nisin improves broiler chicken growth performance and interacts with salinomycin in terms of gastrointestinal tract microbiota composition, Journal of Animal and Feed Sciences, 25, 309–316
Král, M., Angelovičová, M., Alfaig, E., Bučko, O., Walczycka, M., 2014. Influence of Bacillus subtilis and acetic acid on Cobb500 intestinal microflora, Scientific Papers: Animal Science and Biotechnologies, 47(2), 22–25
Lauková, A., Strompfová, V., Plachá, I., Čobanová, K., Faix, S., Simonová, M.P., 2015. Beneficial effect of enterocin M-producing, probiotic strain Enterococcus faecium AL41 in model experiment with hens, Global Journal of Animal Scientific Research, 3(1), 206–213
Loh, T.C., Choe, D.W., Foo, H.L., Sazili, A.Q., Bejo, M.H., 2014. Effects of feding different postbiotic metabolite combinations produced by Lactobacillus plantarum strains on egg quality and production performance, faecal parameters and plasma cholesterol in laying hens, BMC Veterinary Research, 10, 149
National Research Council 1994. Nutrient Requirements of Poultry. 9th rev. ed. National Academy Press, Washington, D.C.
Pan, D. and Yu, Z., 2014. Intestinal microbiome of poultry and its interaction with host and diet, Gut Microbes, 5(1), 108–119
Peyman, F., Yahya, E., Habib, A.S., Naser, M.S., Alireza, A., 2014. Effects of organic acids supplement on performance and gut parameters in male Japanese quail (Coturnix coturnix), Biological Forum – An International Journal, 6(2), 127–134
Salim, H.M., Kang, H.K., Akter, N., Kim, D.W., Kim, J.H., Kim, M.J., Na, J.C., Jong, H.B., Choi, H.C., Suh, O. S., Kim, W.K., 2013. Supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens, Poultry Science, 92(8), 2084–2090
Salmanzadeh, M., Bostanabad, J.G., Arva, S., 2014. The effects of in ovo injection of butyric acid into quails breeder eggs on hatchability, growth performance, development of the gastrointestinal tract, and carcass traits of Japanese quails, Bulletin of Environment, Pharmacology and Life Sciences, 3(3), 126–130
SAS 1999. The SAS System SAS Institute Inc., Cary, NC, USA, Version 8 Copyright © 1999.
Schyns, G., Serra, C.R., Henriques, A.O., Arguelles-Arias, A., Joris, B., Fickers, P., 2013. Isolation of the antimicrobial cyclic peptide subtilosin a from a gut-associated Bacillus subtilis strain, American Journal of Biochemistry and Biotechnology, 9(3), 307–317
Seifi, S., Sayrafi, R., Khoshbakht, R., Gilani, A., 2015. Effects of dietary acetic acid on intestinal microbiota, serum components, internal organs and performance of broilers, Global Journal of Animal Scientific Research, 3(2), 536–543
Shen, X., Yi, D., Ni, X., Zeng, D., Jing, B., Lei, M., Bian, Z., Zeng, Y., Li, T. and Xin, J., 2014. Effects of Lactobacillus plantarum on production performance, immune characteristics, antioxidant status, and intestinal microflora of bursin-immunized broilers, Canadian Journal of Microbiology, 60, 193–202
Song, J., Xiao, K., Ke, Y.L., Jiao, L.F., Hu, C.H., Diao, Q.Y., Shi, B., Zou, X.T., 2014. Effect of probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress, Poultry Science, 93(3), 581–588
Stern, N.J., Svetoch, E.A., Eruslanov, B.V., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Pokhilenko, V.D., Levchuk, V.P., Svetoch, O.E., Seal, B.S., 2006. Isolation of a Lactobacillus salivarius strain and purification of its bacteriocin, which is inhibitory to Camphylobacter jejuni, Antimicrobial Agents and Chemotherapy, 50(9), 3111–3116
Sultan, A., Ullah, T., Khan, S., Khan, R.U., 2015. Effect of organic acid supplementation on the performance and ileal microflora of broiler during finishing period, Pakistan Journal of Zoology, 47(3), 635–639
Thanh, N.T., Loh, T.C., Foo, H.L., Hair-Bejo, M., Azhar, B.K., 2009. Effects of feeding metabolite combinations produced by Lactobacillus plantarum on growth performance, faecal microbial population, small intestine villus height and faecal volatile fatty acids in broilers, British Poultry Science, 50(3), 298–306
Thompson, K.L. and Applegate, T.J., 2006. Feed withdrawal alters small-intestinal morphology and mucus of broilers, Poultry Science, 85, 1535–1540
Wang, H.T., Shih, W.Y., Chen, S.W., Wang, S.Y., 2015. Effect of yeast with bacteriocin from rumen bacteria on laying performance, blood biochemistry, faecal microbiota and egg quality of laying hens, Journal of Animal Physiology and Animal Nutrition, 99(6), 1105–1115
This study was financed under a project supported by the Scientific Research Projects Fund of Adnan Menderes University (project no: ZRF-12016).
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Onder Ustundag, A., Ozdogan, M. Effects of bacteriocin and organic acid on growth performance, small intestine histomorphology, and microbiology in Japanese quails (Coturnix coturnix japonica). Trop Anim Health Prod 51, 2187–2192 (2019). https://doi.org/10.1007/s11250-019-01931-0
- Organic acid
- Growth performance
- Intestinal parameters