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Antimicrobial resistance and presence of virulence factor genes in Trueperella pyogenes isolated from pig lungs with pneumonia

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Abstract

Trueperella pyogenes (T. pyogenes) is a worldwide known pathogen of domestic ruminants and pigs causing a wide variety of infections. The objective of this study was to report the presence of major virulence genes in T. pyogenes isolated from pigs with respiratory clinical signs and determine their resistance to antibiotics at the same time. A total of 27 T. pyogenes strains were obtained from Jilin Province, and the nanH, nanP, cbpA, fimC, and fimE virulence genes were detected in 7 (25.9%), 14 (51.9%), 18 (66.7%), 8 (29.6%), and 16 (59.3%) isolates, respectively. All isolates were observed to harbor plo and fimA genes. However, 27 T. pyogenes strains tested negative for fimG gene. Antibiotic susceptibility tests revealed that the isolated strains had extensive drug resistance, all isolates were sensitive to fluoroquinolones and penicillins antibiotics, and high levels of resistance were found to gentamicin (77.8%), amikacin (74.1%), erythromycin (85.2%), and azithromycin (85.2%). These results highlights the need for prudent use of specific antimicrobial agents in veterinary clinical treatment.

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Acknowledgements

We thank Zhi-chao ZHAI and Jie LIU for their laboratory contributions.

Funding

This research was funded by National Key Research and Development Plan (Grant number 2016YFD0501301), National Natural Science Foundation of China (Grant number 31702293), the Science and Technology Development Plan of Jilin Province (Grant number 20170520074JH), Jilin Provincial Education Department “the 13th five-year” Science and Technology Project (Grant number JJKH20180696KJ), and Jilin Agricultural University Research Foundation (Grant number 201702).

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Correspondence to Ling-Cong Kong or Hong-Xia Ma.

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The authors declare that they have no conflicts of interest.

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All procedures performed with the animals of the present experiment were in accordance with the ethical standards established by the committee of Jilin Agriculture University.

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Dong, WL., Liu, L., Odah, K.A. et al. Antimicrobial resistance and presence of virulence factor genes in Trueperella pyogenes isolated from pig lungs with pneumonia. Trop Anim Health Prod 51, 2099–2103 (2019). https://doi.org/10.1007/s11250-019-01916-z

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