Tropical Animal Health and Production

, Volume 51, Issue 7, pp 2091–2094 | Cite as

Suspected resistance of Trypanosoma species to diminazene aceturate on a cattle farm in Nigeria

  • Paul Olalekan OdeniranEmail author
  • Ewan Thomas Macleod
  • Isaiah Oluwafemi Ademola
  • Susan Christina Welburn
Short Communications


African animal trypanosomiasis is a major cause of mortality and economic losses for the livestock industry in Nigeria. Chemotherapy has been the most reliable option for cattle herders, and the most commonly found drug on the market is diminazene aceturate. To ascertain the long-term efficacy of this compound, we sampled a cattle herd in Ogun State, Nigeria, 2 months after they were treated with diminazene aceturate. The ITS-PCR results revealed 19 positives for trypanosome DNA out of the 79 samples tested (24.1%, 95% CI 16.0–34.5). Seventeen out of the total 19 positives were Trypanosoma congolense (21.5%, 95% CI 13.9–31.8). Mixed infections were also observed. Therefore, the persistence of bovine trypanosomiasis at this Nigerian cattle farm despite treatment could be due to diminazene aceturate resistant trypanosomes being present in the herd.


Trypanosoma congolense Diminazene aceturate Nigeria 



is a chelating material used to purify other compounds via ion exchange. It has the ability to bind transition metal ions. It was used to elute DNA from the FTA cards.


deoxyribonucleic acid


deoxynucleotide triphosphates


internal transcribed spacer


Taq™ master-mix- is a trade name for a PCR buffer with “mango” coloration


polymerase chain reaction


ribosomal deoxyribonucleic acid


Thermus aquaticus used for polymerase chain reaction



We appreciate staff and management of CPV, Federal University of Agriculture, Abeokuta. Nigeria. Special gratitude to Professor Adejinmi J. O. for his financial and moral support.

Funding information

This study was financially supported by Commonwealth Scholarship Commission and The University of Edinburgh, UK. Paul O. Odeniran is a Commonwealth scholar, funded by the UK government with reference number NGCN-2016-196. The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical approval

The study was conducted with the permission of the University of Ibadan Animal Ethics Committee (UI-ACUREC/App/12/2016/05) and in line with the guidelines of the committee.


  1. Bengaly, Z., Vitouley, S.H., Somda, M.B., Zongo, A., Teko-Agbo, A., Cecchi, G., Adam, Y., Sidibe, I., Bayala, B., Belem, A.M.G., Van den Abbeele, J. and Delespaux, V. (2018). Drug quality analysis of isometamidium chloride hydrochloride and diminazene diaceturate used for the treatment of African animal trypanosomosis in West Africa. BMC Veterinary Research, 14, 361.CrossRefPubMedPubMedCentralGoogle Scholar
  2. D'Amico, F., Gouteux, J.P., Le Gall, F., Cuisance, D. (1995). Are stable flies (Dipter: Stomoxyinae) vectors of Trypanosoma vivax in the Central African Republic? Veterinary Research 27(2), 161.170.Google Scholar
  3. Delespaux, V. and de Koning, H.P. (2007). Drugs and drug resistance in African trypanosomiasis. Drug Research Updates, 10, 30–50.CrossRefGoogle Scholar
  4. Fox, K.R., Sansom, C.E. and Stevens, M.F. 1990. Footprinting studies on the sequence-selective binding of pentamidine to DNA. FEBS Letters, 266, 150–154.CrossRefPubMedGoogle Scholar
  5. Geerts, S. and Holmes, P.H. (1998). Drug management and parasite resistance in bovine trypanosomiasis in Africa, PAAT Technical Scientific Series 1, FAO.Google Scholar
  6. Kingsley, P. (2015). Inscrutable medicines and marginal markets: tackling substandard veterinary drugs in Nigeria. Pastoralism: Research, Policy and Practices, 5, 2.CrossRefGoogle Scholar
  7. Odeniran, P.O., Macleod, E.T., Ademola, I.O., Welburn, S.C. (2019a). Molecular identification of bovine trypanosomes in relation to cattle sources in southwest Nigeria. Parasitology International, 68, 1–8.CrossRefPubMedGoogle Scholar
  8. Odeniran, P.O., Macleod, E.T., Ademola, I.O., Welburn, S.C. (2019b). Molecular identification of bloodmeal sources and trypanosomes in Glossina spp., Tabanus spp. and Stomoxys spp. trapped on cattle farm settlements in southwest Nigeria. Medical and Veterinary Entomology, doi:
  9. Maser, P., Luscher, A. and Kaminsky, R. 2003. Drug transport and drug resistance in African trypanosomes, Drug Resistance Update, 6, 281–290.CrossRefGoogle Scholar
  10. Njiru, Z.K., Constantine, C.C., Guya, S., Crowther, J., Kiragu, J.M., Thompson, R.C.A. and Davila, A.M.R. (2005). The use of ITS1 rDNA PCR in detecting African trypanosomes. Parasitology Research, 95, 186–192.CrossRefPubMedGoogle Scholar
  11. Picozzi, K., Tilley, A., Fevre, E.M., Coleman, P.G., Magona, J.W., Oditt, M., Eisler, M.C., Welburn, S.C. (2002). The diagnosis of trypanosome infections: applications of novel technology for reducing disease risk. African Journal of Biotechnology, 1, 39–45.CrossRefGoogle Scholar
  12. Sonibare, A.O., Famuyide, I.M., Takeet, M.I., Oyewusi, I.K., Egbetade, A.O., Abapka, S.A.V., Adeniran, O.A., Solanke, A.O., 2016. Diminazene-resistant Trypanosoma vivax in West African dwarf lamb, South-West, Nigeria: a case report. Nigerian Journal of Parasitology 37, 62.CrossRefGoogle Scholar
  13. Sumba, A.L., Mihok, S., Oyieke, FA. (1998). Mechanical transmission of Trypanosoma evansi and T. congolense by Stomoxys niger and S. taeniatus in a laboratory mouse model. Medical Veterinary Entomology, 12(4), 417–422.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Paul Olalekan Odeniran
    • 1
    • 2
    Email author
  • Ewan Thomas Macleod
    • 2
  • Isaiah Oluwafemi Ademola
    • 1
  • Susan Christina Welburn
    • 2
    • 3
  1. 1.Department of Veterinary Parasitology and Entomology, Faculty of Veterinary MedicineUniversity of Ibadan, NigeriaIbadanNigeria
  2. 2.Division of Infection and Pathway MedicineUniversity of EdinburghEdinburghUK
  3. 3.Zhejiang University – University of Edinburgh Joint InstituteZhejiang UniversityHainingChina

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