Vertical transmission of Neospora caninum in bovine fetuses from a slaughterhouse in Brazil

Abstract

Neospora caninum is considered as one of the main causes of reproductive failure in cattle. Vertical transmission is the main route of infection in the bovine host and plays an important role in maintaining the parasite in the herd. Molecular detection of N. caninum is important to determine the occurrence of the disease and to evaluate the genetic diversity of the parasite. The present study aimed at assessing the vertical transmission of N. caninum using molecular techniques to detect the parasite in tissue samples from bovine fetuses collected in a slaughterhouse in the state of São Paulo, Brazil. Seventy fetuses and 70 blood samples from pregnant cows were collected in a slaughtering line. Fresh samples of heart and brain tissue from fetuses were analyzed using molecular assays. Serum samples from fetuses and cows were subjected to an indirect fluorescent antibody test (IFAT) to detect antibodies against N. caninum. Nested PCR targeting the internal transcriber 1 (ITS1) region of the protozoan organism was used in the molecular testing. From the total of fetuses examined, 71.42% were positive for N. caninum by PCR. A higher number of heart samples (47.1%) were positive for the parasite using this technique. Antibodies against the protozoa were detected in 12.9% of serum samples of cows; 2.8% of fetuses were seropositive for this pathogen. Our results show that vertical transmission of N. caninum occurs in cattle from this region of Brazil, and that the use of different diagnostic techniques contributes to successful diagnosis of congenital transmission of the parasite in cattle.

This is a preview of subscription content, log in to check access.

References

  1. Almería, S., Serrano-Pérez, B. and López-Gatius, F., 2017. Immune response in bovine neosporosis: protection or contribution to the pathogenesis of abortion. Microbial Pathogenesis, 109: 177–182.

    Article  CAS  PubMed  Google Scholar 

  2. Al-Qassab, S., Reichel, M.P., Ivens, A. and Ellis, J.T., 2009. Genetic diversity amongst isolates of Neospora caninum, and the development of a multiplex assay for the detection of distinct strains. Molecular and Cellular Probes, 23(3–4): 132–139

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Álvarez-García, G., Pereira-Bueno, J., Gómez-Bautista, M. and Ortega-Mora, L.M., 2002. Pattern of recognition of Neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. Veterinary Parasitology, 107(1–2): 15–27.

    Article  PubMed  Google Scholar 

  4. Brom, P.R.F., Regidor-Cerrillo, J., Collantes-Fernández, E., Ortega-Mora, L.M., Guimarães, M.S. and da Silva, A.C., 2014. Genetic characterisation of Neospora caninum strains from clinical samples of zebuine foetuses obtained in abattoirs in Goiás, Brazil. Veterinary Parasitology, 204(3–4): 381–387.

    Article  PubMed  Google Scholar 

  5. Buxton, D., Maley, S.W., Wright, S., Thomson, K.M., Rae, A.G. and Innes, E.A., 1998. The pathogenesis of experimental neosporosis in pregnant sheep. Journal of Comparative Pathology, 118(4): 267–279.

    Article  CAS  PubMed  Google Scholar 

  6. Campero, L.M., Venturini, M.C., Moore, D.P., Massola, L., Lagomarsino, H., Garcia, B., Bacigalupe, D., Rambeaud, M., Pardini, L., Leunda, M.R., Schares, G. and Campero, C.M., 2015. Isolation and molecular characterization of a new Neospora caninum isolate from cattle in Argentina. Experimental parasitology, 155: 8–12.

    Article  CAS  PubMed  Google Scholar 

  7. Cerqueira-Cézar, C.K., Calero-Bernal, R., Dubey, J.P. and Gennari, S.M., 2017. All about neosporosis in Brazil. Revista Brasileira de Parasitologia Veterinária, 26(3): 253–279.

    Article  CAS  PubMed  Google Scholar 

  8. Conrad, P.A., Sverlow, K., Anderson, M., Rowe, J., Bondurant, R., Tuter, G., Breitmeyer, R., Palmer, C., Thurmond, M., Ardans, A., Dubey, J.P., Duhamel, G. and Barr, B., 1993. Detection of serum antibody responses in cattle with natural or experimental Neospora infections. Journal of Veterinary Diagnostic Investigation, 5(4): 572–578.

    Article  CAS  PubMed  Google Scholar 

  9. de Barros, L.D., Miura, A.C., Minutti, A.F., Vidotto, O. and Garcia, J.L., 2018. Neospora caninum in birds: A review. Parasitology International, 67(4): 397–402.

    Article  PubMed  Google Scholar 

  10. Dubey, J.P. and Schares, G., 2011. Neosporosis in animals-The last five years. Veterinary Parasitology, 180(1–2): 90–108.

    Article  CAS  PubMed  Google Scholar 

  11. Dubey, J.P., Schares, G. and Ortega-Mora, L.M., 2007. Epidemiology and Control of Neosporosis and Neospora caninum. Clinical Microbiology Reviews, 20(2): 323–367.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Dubey, J.P., Jenkins, M.C., Rajendran, C., Miska, K., Ferreira, L.R., Martins, J., Kwok, O.C.H. and Choudhary, S., 2011. Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum. Veterinary Parasitology, 181(2–4): 382–387.

    Article  CAS  PubMed  Google Scholar 

  13. Dubey, J.P., Jenkins, M.C., Ferreira, L.R., Choudhary, S., Verma, S.K., Kwok, O.C.H., Fetterer, R., Butler, E. and Carstensen, M., 2014. Isolation of viable Neospora caninum from brains of wild gray wolves (Canis lupus). Veterinary parasitology, 201(1–2): 150–153.

    Article  CAS  PubMed  Google Scholar 

  14. Garcia, J.L., Gennari, S.M., Machado, R.Z. and Navarro, I.T., 2006. Toxoplasma gondii: Detection by mouse bioassay, histopathology, and polymerase chain reaction in tissues from experimentally infected pigs. Experimental Parasitology, 113(4): 267–271.

    Article  CAS  PubMed  Google Scholar 

  15. Gondim, L.F.P., Mcallister, M.M., Pitt, W.C. and Zemlicka, D.E., 2004. Coyotes (Canis latrans) are definitive hosts of Neospora caninum. International Journal for Parasitology, 34(2): 159–161.

    Article  PubMed  Google Scholar 

  16. González-Warleta, M., Castro-Hermida, J.A., Calvo, C., Pérez, V., Gutiérrez-Expósito, D., Regidor-Cerrillo, J., Ortega-Mora, L.M. and Mezo, M., 2018. Endogenous transplacental transmission of Neospora caninum during successive pregnancies across three generations of naturally infected sheep. Veterinary Research, 49(1): 106.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Guedes, M.H.P., Guimarães, A.M., Rocha, C.M.B.M. and Hirsch, C., 2008. Frequência de anticorpos anti-Neospora caninum em vacas e fetos provenientes de municípios do sul de Minas Gerais. Revista Brasileira de Parasitologia Veterinaria, 17(4): 189–194.

    Article  PubMed  Google Scholar 

  18. Guido, S., Katzer, F., Nanjiani, I., Milne, E. and Innes, E.A., 2016. Serology-Based Diagnostics for the Control of Bovine Neosporosis. Trends in Parasitology, 32(2): 131–143.

    Article  PubMed  Google Scholar 

  19. King, J.S., Šlapeta, J., Jenkins, D.J., Al-Qassab, S.E., Ellis, J.T. and Windsor, P.A., 2010. Australian dingoes are definitive hosts of Neospora caninum. International Journal for Parasitology, 40(8): 945–950.

    Article  PubMed  Google Scholar 

  20. Marques, F.A.C., Headley, A.S., Figueredo-Pereira, V., Taroda, A., Barros, L.D., Cunha, I.A.L., Munhoz, K., Bugni, F.M., Zulpo, D.L., Igarashi, M., Vidotto, O., Guimarães, J.S. and Garcia, J.L., 2011. Neospora caninum: Evaluation of vertical transmission in slaughtered beef cows (Bos indicus). Parasitology Research, 108(4): 1015–1019.

    Article  CAS  PubMed  Google Scholar 

  21. McAllister, M.M., Dubey, J.P., Lindsay, D.S., Jolley, W.R., Wills, R. a. and McGuire, A.M., 1998. Dogs are definitive hosts of Neospora caninum. International Journal for Parasitology, 28(9): 1473–1478.

    Article  CAS  PubMed  Google Scholar 

  22. Pereira-Bueno, J., Quintanilla-Gozalo, A., Pérez-Pérez, V., Espi-Felgueroso, A., Álvarez-Garcı́a, G., Collantes-Fernández, E. and Ortega-Mora, L.M., 2003. Evaluation by different diagnostic techniques of bovine abortion associated with Neospora caninum in Spain. Veterinary Parasitology, 111(2–3): 143–152.

    Article  CAS  PubMed  Google Scholar 

  23. Reichel, M.P., Alejandra Ayanegui-Alcérreca, M., Gondim, L.F.P. and Ellis, J.T., 2013. What is the global economic impact of Neospora caninum in cattle - the billion dollar question. International journal for parasitology, 43(3–2): 133–142.

    Article  PubMed  Google Scholar 

  24. Venturini, M.C., Venturini, L., Bacigalupe, D., Machuca, M., Echaide, I., Basso, W., Unzaga, J.M., Di Lorenzo, C., Guglielmone, A., Jenkins, M.C. and Dubey, J.P., 1999. Neospora caninum infections in bovine foetuses and dairy cows with abortions in Argentina International Journal for Parasitology, 29(10):1705–1708

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

We would like to thank the Fundação de Amparo à Pesquisa do Estado de São Paulo for financial support (FAPESP-IC no. 2016/10938-4).

Author information

Affiliations

Authors

Corresponding author

Correspondence to Luiz Daniel de Barros.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

de Aquino Diniz, L.V., Minutti, A.F., de Souza Lima Nino, B. et al. Vertical transmission of Neospora caninum in bovine fetuses from a slaughterhouse in Brazil. Trop Anim Health Prod 51, 1751–1755 (2019). https://doi.org/10.1007/s11250-019-01828-y

Download citation

Keywords

  • Neosporosis
  • IFAT
  • PCR
  • Congenital transmission