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Effect of dietary inclusion of small quantities of Mucuna pruriens seed meal on sexual behavior, semen characteristics, and biochemical parameters in rabbit bucks (Oryctolagus cuniculus)

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Abstract

The aim of this work was to investigate the effects of Mucuna pruriens seed meal (MSM) on sexual behavior, semen, and biochemical parameters in rabbit bucks. Twenty-four 12-week-old rabbit bucks weighing 1002 to 1156 g were randomly allocated to three experimental diets containing 0, 1.5, and 3% of MSM in a 3-month trial. Sexual behavior parameters such as mounting latency, mounting frequency, successful mounting frequency, intromission latency, and post ejaculatory interval were monitored at the end of the experiment by mating with receptive females. Thereafter, rabbits were weighed, stunned, and humanely sacrificed and testes, epididymis, and vas deferens were harvested for evaluation of organ weights and semen characteristics. Results indicate that supplementing rabbit diet with MSM induced a significant decrease (P < 0.05) in mounting latency (69.7%) and intromission latency (19.7%), while it significantly (P < 0.05) increased successful mounting frequency (60%) as well as relative weight of testis (33.3%) and vas deferens (54.5%). There was a dose-dependent increase (P < 0.05) in sperm motility (35.7%) and concentration (65.9%), serum albumin (19.1%) and protein concentration (9.9%), and a decrease in sperm morphological alterations (68.3%), serum cholesterol (13.4%), and urea (11.6%) in treatment groups where MSM was supplemented at 3% compared to controls. From the findings, it appears MSM is a potential enhancer of male reproductive performance that can be recommended to rabbit farmers for improving reproductive performance and quality of semen, hence a boon to reproduction and production in rabbit farming industry.

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Acknowledgements

The authors would like to acknowledge the Université Evangélique en Afrique, Bukavu, DR Congo, for providing technical and laboratory supports. Dr. B. L. Maass, Germany, is thanked for reviewing an earlier draft of this article.

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Authors and Affiliations

  1. Department of Animal Production, Faculty of Agriculture and Environmental Studies, Université Evangélique en Afrique (UEA), Bukavu, Democratic Republic of the Congo

    V. B. Mutwedu, R. B. B. Ayagirwe, L. M. Mwema & S. Butseme

  2. International Livestock Research Institute (ILRI), Bukavu, Democratic Republic of the Congo

    V. B. Mutwedu

  3. International Institute of Tropical Agriculture (IITA), Bukavu, Democratic Republic of the Congo

    S. B. Bacigale

  4. Bio-Medical Laboratory, Université Evangélique en Afrique (UEA), Bukavu, Democratic Republic of the Congo

    T. Kashosi & B. Mitima

  5. International Livestock Research Institute (ILRI), Bujumbura, Burundi

    G. J. Manyawu

  6. Department of Veterinary Anatomy and Physiology, University of Nairobi, Nairobi, Kenya

    A. W. Nyongesa

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  1. V. B. Mutwedu
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  2. R. B. B. Ayagirwe
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Correspondence to V. B. Mutwedu.

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The experimental protocol was approved by the Ethical Committee of the Université Evangélique en Afrique, and the experiments were performed in accordance with the internationally accepted standard ethical guidelines for laboratory animal use and care as described in the European Community guidelines; EEC Directive 86/609/EEC, of the 24th November 1986.

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Mutwedu, V.B., Ayagirwe, R.B.B., Bacigale, S.B. et al. Effect of dietary inclusion of small quantities of Mucuna pruriens seed meal on sexual behavior, semen characteristics, and biochemical parameters in rabbit bucks (Oryctolagus cuniculus). Trop Anim Health Prod 51, 1195–1202 (2019). https://doi.org/10.1007/s11250-019-01808-2

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