The aim of this study was to evaluate the effect of a co-treatment of follicle-stimulating hormone (FSH) plus equine chorionic gonadotrophin (eCG) on serum insulin and insulin-like growth factor 1 (IGF-1) concentrations, superovulatory response, ovulatory rate, and number and production of embryos in Katahdin breed ewes during the non-breeding season. Twenty Katahdin ewes were synchronized with progestagens (CIDR) and assigned to two superovulation treatments (n = 10): (1): ewes treated with 200 mg ewe−1 of FSH from day 5 to 8 after CIDR insertion at decreasing doses every 12 h (FSH group) and (2) ewes treated as FSH group plus 300 IU of eCG on day 5 after CIDR insertion (FSH + eCG group). Estrous behavior was monitored and direct mating was performed. On days − 7 (CIDR insertion), 0 (CIDR withdrawal), and 7 (embryo recovery), blood samples were collected to determine serum hormone concentrations. Co-treatment with eCG (FSH group) did not affect (P > 0.05) serum hormone levels. Superovulation response, ovulation rate, recovery rate, fertilization, and number of embryos were also similar (P > 0.05) between treatments. Compared with FSH group, FSH + eCG ewes had lower (P < 0.05) number of transferable embryos and higher (P < 0.05) number of oocyte and a tendency to increase the number of degenerated embryos (P = 0.07). Overall results suggest that the administration of eCG is not beneficial either to improve the ovulatory response or the amount of transferable embryos in Katahdin ewes superovulated with a protocol using progesterone and FSH at decreasing doses.
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The authors would like to thank the Faculty Improvement Program (PROMEP) for financing project UJAT-CA-221, as well as Mr. H. Pérez for granting the ewes for this experiment.
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The authors declare that they have no conflicts of interest. They also confirm that this manuscript has been read and that all of them have approved the order of authors listed in the manuscript.
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Animal handling procedures were performed in accordance with the Mexican Official Standard (NOM-051-ZOO-1995: Humanitarian care of animals during its mobilization, and NOM-062-ZOO-1999: Technique specifications to production, care, and use of laboratory animals), and procedures of Ethics Committee of the University Juarez Autonoma of Tabasco.
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Luna-Palomera, C., Macías-Cruz, U. & Sánchez-Dávila, F. Superovulatory response and embryo quality in Katahdin ewes treated with FSH or FSH plus eCG during non-breeding season. Trop Anim Health Prod 51, 1283–1288 (2019). https://doi.org/10.1007/s11250-019-01801-9
- Ovarian response
- Katahdin ewes