Tropical Animal Health and Production

, Volume 51, Issue 4, pp 905–910 | Cite as

Comparative effectiveness of Sumaq and Neem extract cream, Eniloconazole and glycerine iodine on dermatophytosis in Arabian horses: a randomized clinical trial

  • Mohamed Z. Sayed-AhmedEmail author
  • Ahmed M. Ahdy
  • Emad E. Younis
  • Sabry A. El-khodery
  • Hany N. Baraka
Regular Articles


Dermatophytosis is a contagious fungal disease among animal communities. The major concerns of dermatophytosis are cost of treatment, difficulty of control, and the public health consequences. The objective of the present study was to compare the clinical efficacy of Sumaq and Neem extract cream with that of traditional treatments, eniloconazole and glycerine iodine, on dermatophytosis in Arabian horses. For this purpose, 37 Arabian horses with dermatophytosis had been used. Fungal isolation and identification for each horse were carried out by standard microbiological procedures. Sumaq (Rhus coriaria Linn.) and Neem (Azadirachta indica) seed extracts were selected based on in vitro effectiveness. Horses were randomly allocated into four groups. The first group (n = 8) was treated with glycerine iodine 10% daily for 7 days. The second group (n = 10) was treated using eniloconazole 10% emulsified concentrate four times with 2 days interval. The third group (n = 11) was treated with Neem seeds extract (20% cream) daily for 10 days, and the fourth group (n = 8) was treated with Sumaq extract (5% cream) daily for 10 days. Clinical and mycological evaluations were assessed at 0, 7, 14, 21, and 28 days post-treatment. The Sumaq and eniloconazole were more effective on clinical index score after 7 days of treatment followed by Neem seed 20% cream. At 28 days post-treatment, a complete healing was obtained by Sumaq 5% cream and eniloconazole (median clinical sum score = zero) followed by Neem (median clinical sum score = 1.5). Moreover, Dermatophytes spp. was isolated only from two horses, one from Neem group and the other from glycerin iodine group. The present results indicate that Sumaq 5% and Neem seed extract are effective alternative treatment for dermatophytosis in Arabian horses.


Arabian horses Dermatophytosis Egypt Herbal extracts Treatment 


Compliance with ethical standards

Ethical approval

This study protocol was approved by the Committee of Animal Welfare and Ethics, Faculty of Veterinary Medicine, Mansoura University, Egypt.

Competing interests

The authors declare that they have no competing interests.


  1. Ahdy, M.M., Sayed-Ahmed, M.Z., Younis, E.E., Baraka, H.N., & El-khodery, S.A. (2016). Prevalence and Potential Risk Factors of Dermatophytosis in Arabian Horses in Egypt. Journal of Equine Veterinary Science, 37, 71–76.CrossRefGoogle Scholar
  2. Araujo, C.R., Miranda, K.C., Fernandes, O.F.L., Soares A.J., Silva M.R.R. 2009. In vitro susceptibility testing of dermatophytes isolated in Goiania, Brazil, against five antifungal agents by broth microdilution method. Revista do Instituto de Medicina Tropical de São Paulo., 51, 9–12.CrossRefPubMedGoogle Scholar
  3. Awad, W.S.A, 1995. Some studies on some infectious skin diseases in animals. M.V.Sc Thesis (Infectious diseases) Faculty of Veterinary Medicine, Cairo University.Google Scholar
  4. Biswas, K., Chattopadhyay I., Banerjee, R.K. & Bandyopadhyay, U. 2002. Biological activities and medicinal properties of neem (Azadirachta indica). Review article. Current Science, 82, 10.Google Scholar
  5. Consalvi, P.J. 1983. L’énilconazole: une nouvelle molecule utilisable dans le traitement par voie externe des dermatomycoses des chiens, des chevaux et des bovins. Pratique Médicale et Chirurgicale de l'Animal de, 18, 16.Google Scholar
  6. Fardos, M.B., 2009. Antifungal activity of some medicinal plants used in Jeddah, Saudi Arabia. Mycopath, 7, 51–57.Google Scholar
  7. Harding, R.B., 1981. Treatment of ringworm in the horse with griseofulvin. Veterinary. Dermatology News, 6, 40.Google Scholar
  8. Hiddleston, W.A., 1970. The use of griseofulvin mycelium in equine animals. Veterinary Record, 86, 75.CrossRefGoogle Scholar
  9. Kumar, S.M., Kumar, V.A., Natarajan, P., Sreenivasan, G., 2018. Antifungal Efficacy and the Mechanical Properties of Soft Liners against Candida albicans after the Incorporation of Garlic and Neem: An In vitro Study. Journal of International Society of Preventive Community Dentistry. 8,:212–217.CrossRefPubMedPubMedCentralGoogle Scholar
  10. Mahmoud, D,A, Hassanein, N.M., Youssef, K.A., Abou Zeid, M.A., 2011. Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens. Brazilian Journal of Microbiology, 42, 1007–1016Google Scholar
  11. Maurice, M.N., Kazeem, H.M., Kwanashie, C.N., Maurice, N.A., Ngbede, E.O., Adamu, H.N., Mshelia, W.P., Edeh, R.E. 2016. Equine Dermatophytosis: A Survey of Its Occurrence and Species Distribution among Horses in Kaduna State, Nigeria. Scientifica (Cairo). 2016;2016:6280646. doi: Scholar
  12. McCutcheon, A.R., Ellis, S.M., Hancock, R.E., Towers, G.H., 1994. Antifungal screening of medicinal plants of British Columbia native peoples. Journal of Ethnopharmacology, 44, 157–169.CrossRefPubMedGoogle Scholar
  13. Moriello, K. A., DeBoer, D.J., Semrad, S. D., 1998. Diseases of the skin. In: Reed SM, Bayly WM (eds): Equine internal medicine W.B. Saunders Co, Philadelphia, USA. p 513.Google Scholar
  14. Moretti, A., Agnetti, F., Mancianti, F., Nardoni, S., Righi, C., Moretta, I., Morganti, G., Papini, M. 2013. Dermatophytosis in animals: epidemiological, clinical and zoonotic aspects. Giornale italiano di dermatologia e venereologia, 148, 563–72.PubMedGoogle Scholar
  15. Natarajan, V., Venugopal, P., Menon, T., 2003. Effect of Azadirachta indica (neem) on the growth pattern of dermatophytes. Indian Journal of Medical Microbiology, 21, 98–101.PubMedGoogle Scholar
  16. Pascoe R.R., 1979. The epidemiology of ringworm in racehorses caused by Trichophyton equinum var autotrophicum. Australian Veterinary Journal, 55, 403–407.CrossRefPubMedGoogle Scholar
  17. Pascoe, R.R., 1984. Experimental medication of equine ringworm due to Trichophyton equinum var. autotrophycum. Australian Veterinary Journal, 61, 231–235.CrossRefPubMedGoogle Scholar
  18. Pascoe, R.R.R., Knottenbelt, D.C. 1999. Manual of Equine Dermatology. W.B. Saunders Co, Philadelphia.Google Scholar
  19. Paterson, S., 1997. Dermatophytosis in 25 horses-a protocol of treatment using topical therapy. Equine Veterinary Education, 9, 171.CrossRefGoogle Scholar
  20. Pisseri, F., Bertoli, A., Nardoni, S., Pinto, N., Pistelli, L., Guidi, G., Mancianti, F., 2009. Antifungal activity of tea tree oil from Melalesuca alternifolia against Trichophyton equinum: An in vivo assay. Journal of Phytomedicine, 16, 1056–1058.CrossRefPubMedGoogle Scholar
  21. Radostitis, O.M., Gay, C.C., Hinchcliff, K.W., Constable P.D., 2006. Veterinary Medicine, 10th Ed., El Sevier, 1476–1478.Google Scholar
  22. Rosychuk R, Yu, A., 2013. Equine dermatology, an issue of veterinary clinics: equine practice. New York, USA: Elsevier Health Sciences.Google Scholar
  23. Scott, D.W., 1988. Large Animal Dermatology. W.B. Saunders Co., Philadelphia, USA.Google Scholar
  24. Scott D.W., Miller W.H., Griffin, C. E., 2001. Muller and Kirk’s Small Animal Dermatology, 6th ed. W.B. Saunders Co, Philadelphia, USA.Google Scholar
  25. Scott, D.W., Miller, W.H., 2003. Equine Dermatology, Saunders, Elsevier Science, USA.Google Scholar
  26. Sierra, R., Mazza, G., 2007. Biological activities of extracts from sumac (Rhus spp.): a review. Plant Foods for Human Nutrition (Dordrecht, Netherlands), 62, 165–75.CrossRefGoogle Scholar
  27. Tartor, Y. H, El Damaty, H. M., Mahmmod, Y.S., 2016. Diagnostic performance of molecular and conventional methods for identification of dermatophyte species from clinically infected Arabian horses in Egypt. Veterinary Dermatology, 27, 401.CrossRefPubMedGoogle Scholar
  28. Thomas P., Korting H.C., Strassl W. Ruzicka T., 1994. Microsporum canis infection in a 5-year-old boy: transmission from the interior of a second-hand car. Mycoses 37, 141–142.CrossRefPubMedGoogle Scholar
  29. Ural, K., Ulutas, B., 2008. Immunization with Trichophyton verrucosum Vaccine in Hunter/Jumper and Dressage Horses with Naturally Occurring Trichophyton equinum Infection: A Prospective, Randomized, Double- Blinded, Placebo-Controlled Clinical Trial. Journal of Equine Veterinary Science, 28, 590–593.CrossRefGoogle Scholar
  30. Walid, S.A.A., 1995. Studies on some infectious skin diseases in animals, M.V.Sc. (Infectious Diseases), Faculty of veterinary medicine, Cairo University.Google Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  2. 2.Department of Clinical Pharmacy, Faculty of PharmacyJazan UniversityJazanSaudi Arabia
  3. 3.Veterinarian at Elzahraa State studGizaEgypt
  4. 4.Department of Pharmacognosy, Faculty of PharmacyMansoura UniversityMansouraEgypt

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