Tropical Animal Health and Production

, Volume 51, Issue 4, pp 847–852 | Cite as

The first molecular detection of Clostridium perfringens from pneumonic cases associated with foot and mouth disease in cattle and buffalo in Egypt

  • Magdy Elgioushy
  • Mohamed Abdo Rizk
  • Mohamed El-Adl
  • Mohamed Elhadidy
  • Sabry El-KhoderyEmail author
Regular Articles


Panting syndrome and respiratory infection have been recorded in complicated cases of foot and mouth disease (FMD) in cattle. However, investigations on the causative agents of respiratory disease in such cases are scarce. In this study, 30 animals (13 buffalo and 17 cattle) suffering from respiratory distress associated with signs of FMD were examined. Serum samples were collected and FMD infection was confirmed. Bacteriological examination of lungs from eight necropitized cases revealed the presence of C. perfringens. Multiplex polymerase chain reaction (mPCR) was performed on the positive samples followed by sequencing analysis. The alpha toxin gene (plc) of C. perfringens was identified in six cases. The present investigation highlights the role of clostridial infection as a complication of FMD in cattle and buffalo. This is the first report identifying the C. perfringens toxins from lung of animals with respiratory distress associated with FMD infection.


FMD Clostridium perfringens Egypt Alpha toxin 


Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Ethical disclosure

Farm owners approved and signed the informed consent forms for the study. All animal procedures implemented in this study complied with institutional guidlines obtained from ethics committee in Mansoura University, Egypt.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Animal Medicine, Faculty of Veterinary MedicineAswan UniversityAswanEgypt
  2. 2.Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  3. 3.Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt
  4. 4.Zewail City of Science and TechnologyUniversity of Science and TechnologyGizaEgypt
  5. 5.Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary MedicineMansoura UniversityMansouraEgypt

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