Antimicrobial susceptibility and molecular typing of Pasteurella multocida isolated from six provinces in China

Abstract

Pasteurella multocida (P. multocida) is an important pathogen that causes bovine respiratory disease (BRD) in China and other countries. To investigate the antimicrobial susceptibility of P. multocida isolated from different provinces in China, we analyzed antimicrobial susceptibility phenotypes and pulsed-field gel electrophoresis (PFGE) types of P. multocida; then, we sequenced the complete genome of strain found to be multidrug-resistant. The isolates exhibited resistance to many antimicrobial agents, especially amikacin, sulfamethoxazole, sulfachloropyridazinesodium, macrolides, and fluoroquinolones. Pulsed-field gel electrophoresis analysis showed that a clonal spread of multidrug-resistant isolates occurred in various provinces. All of the isolates carried class I integron.

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Acknowledgements

We thank Ying Zhang, Lei Liu, and Ren-ge Hu for their laboratory contributions.

Funding

This research was funded by the National Key Research and Development Plan (grant number: 2016YFD0501301), the National Natural Science Foundation of China (grant number: 31702293), the Science and Technology Development Plan of Jilin Province (grant number: 20170520074JH), Jilin Provincial Education Department “the 13th five-year” Science and Technology Project (grant number: JJKH20180696KJ), and the Jilin Agricultural University Research Foundation (grant number: 201702).

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Correspondence to Xiu-Yun Jiang or Hong-Xia Ma.

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Statement of animal rights

This study was carried out in accordance with the recommendations of ‘Laboratory animal-Guideline for ethical review of animal welfare (GB/T 35892-2018), General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China’. The protocol was approved by the Animal Care and Use Committee of the Jilin Agricultural University.

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The authors declare that they have no conflict of interest.

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Kong, LC., Wang, Z., Wang, YM. et al. Antimicrobial susceptibility and molecular typing of Pasteurella multocida isolated from six provinces in China. Trop Anim Health Prod 51, 987–992 (2019). https://doi.org/10.1007/s11250-018-1754-9

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Keywords

  • Minimal inhibitory concentration
  • Pasteurella multocida
  • Molecular typing