Abstract
High-quality protein roughage is an important feed for productive ruminants. This study examined the effects of strategic feeding of lactating cows with cassava (Manihot esculenta) top silage (CTS) on rumen fermentation, feed intake, milk yield, and quality. Four early lactating crossbred dairy cows (75% Holstein-Friesian and 25% Thai) with body weight (BW) 410 ± 30 kg and milk yield 12 ± 2 kg/day were randomly allotted in a 4 × 4 Latin square design to four different supplementation levels of CTS namely, 0, 0.75, 1.50, and 2.25 kg/day of dry matter (DM). Strategic supplementation of CTS significantly affected ruminal fermentation end-products, especially increased propionate production, decreased protozoal population and suppressed methane production (P < 0.05). Increasing the CTS supplementation level substantially enhanced milk yield and the 3.5% FCM from 12.7 to 14.0 kg/day and from 14.6 to 17.2 kg/day (P < 0.05) for non-supplemented group and for the 2.25 kg/day supplemented group, respectively. We conclude that high-quality protein roughage significantly enhances rumen fermentation end-products, milk yield, and quality in dairy cows.
Similar content being viewed by others
References
Aminah, A., Abu, B.C., and Izham, A. 1999. Silages from tropical forages: nutritional quality and milk production. FAO Electronic Conference on Tropical Silage. Rome. http://www.fao.org.
AOAC. 2012. Official Methods of Analysis, 19th ed. Association of Official Analytical Chemists, Gaithersburg, MD.
Bradbury, J.H., Egan, S.V., and Lynch, M.J. 1991. Analysis of cyanide in cassava using acid hydrolysis of cyanogenic glucosides. Journal of the Science of Food and Agriculture, 55, 277–290.
Broderick, G.A., Huhtanen, P., Ahvenjärvi, S., Reynal, S.M., and Shingfield, K.J. 2010. Quantifying ruminal nitrogen metabolism using the omasal sampling technique in cattle-A meta-analysis. Journal of Dairy Science, 93, 3216–3230.
Cao, Y., Takahashi, T., and Horiguchi, K. 2009. Effects of addition of food by products on the fermentation quality of a total mixed ration with whole crop rice and its digestibility, preference, and rumen fermentation in sheep. Animal Feed Science and Technology, 151, 1–11.
Carpintero, C.M., Henderson, A.R., and McDonald, P. 1979. The effect of some pre-treatments on proteolysis during the ensiling of herbage. Grass and Forage Science, 34, 311–315.
Cheli, F., Campagnoli, A., and Dell’Orto, V. 2013. Fungal populations and mycotoxins in silages: from occurrence to analysis. Animal Feed Science and Technology, 183, 1–16.
Crocker, C.L. 1967. Rapid determination of urea nitrogen in serum or plasma without deproteinization. The American Journal of Medical Technology, 33, 361–365.
Denman, S.E., and McSweeney, C.S. 2006. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology, 58, 572–582.
Doyle, P.T, Devendra, C., and Pearce, G.R. 1986. Rice straw as a feed for ruminants. International Development Program of Australian Universities and Colleges Limited (IDP), Canberra, Australia.
Flaten, O., Asheim, L.J., Dønnemb, I., and Lunnan, T. 2012. The profitability of early grass silage harvesting on dairy goat farms in mountainous areas of Norway. Small Ruminant Research, 103, 133–142.
Galyean, M., 1989. Laboratory Procedure in Animal Nutrition Research. Department of Animal and Range Sciences, New Mexico State University, New Mexico.
Ghanbari, F., Ghoorchi, T., Shawrang, P., Mansouri, H., and Torbati-Nejad, N.M. 2012. Comparison of electron beam and gamma ray irradiations effects on ruminal crude protein and amino acid degradation kinetics, and in vitro digestibility of cottonseed meal. Radiation Physics and Chemistry, 81, 672–678.
Giang, N.T.T., Wanapat, M., Phesatcha, K., and Kang, S., 2016. Level of Leucaena leucocephala silage feeding on intake, rumen fermentation and nutrient digestibility in dairy steers. Tropical Animal Health and Production, 48(5), 1057–1064.
Herrero, M., Havlik, P., Valin, H., Notenbaert, A., Rufino, M, Thornton, P.K., Blummel, M., Weiss, F., and Obersteiner, M. 2013. Global livestock systems: biomass use, production, feed efficiencies and greenhouse gas emissions. Proceedings of the National Academy of Sciences of the United States of America, 110(52), 20888–20893.
Hobson, P.N., and Stewart, C.S. 1997. The rumen microbial ecosystem. Chapman and Hall, New York: 719.
Huhtanen, R., Rinne, M., and Nousiainen, J. 2007. Evaluation of the factors affecting silage intake of dairy cows: a revision of the relative silage dry-matter intake index. Animal, 1, 758–770.
Johnson, J.M., Franzluebbers, A.J., Weyers, S.L., and Reicosky, D.C. 2007. Agricultural opportunities to mitigate greenhouse gas emissions. Environmental Pollution, 150(1), 107–124.
Kamra, D.N. 2005. Rumen microbial ecosystem. Current Science, 89, 124–135.
Kanjanapruthipong, J., and Leng, R.A. 1998. The effects of dietary urea on microbial populations in the rumen of sheep. Asian–Australasian Journal of Animal Science, 11(6), 661–672.
Khang, D.N., and Wiktorsson, H. 2004. Effects of fresh cassava tops on rumen environment parameters, thyroid gland hormones and liver enzymes of local yellow cattle fed urea-treated fresh rice straw. Tropical Animal Health and Production, 36, 751–762.
Khang, D.N., Wiltorsson, H., and Preston, T.R. 2005. Yield and chemical composition of cassava foliage and tuber yield as influenced by harvesting height and cutting interval. Asian–Australasian Journal of Animal Science, 18, 1029–1035.
Kholif, A.E., Khattab, H.M, El-Shewy, A.A., Salem, A.Z.M., Kholif, A.M., El-Sayed, M.M., Gado, H.M., and Mariezcurrena, M.D. 2014. Nutrient digestibility, ruminal fermentation activities, serum parameters and milk production and composition of lactating goats fed diets containing rice straw treated with Pleurotus ostreatus. Asian–Australasian Journal of Animal Science, 27, 357–364.
Liu, J.J., Liu, X.P., Ren J.W., Zhao, H.Y., Yuan, X.F., Wang, X.F., Abdelfattah, Z.M.S., and Cui, Z.J. 2015. The effects of fermentation and adsorption using lactic acid bacteria culture broth on the feed quality of rice straw. Journal of Integrative Agriculture, 14(3), 503–513.
Man, N.V., and Wiktorsson, H. 2001. Cassava tops ensiled with or without molasses as additive effects on quality, feed intake and digestibility by heifers. Asian–Australasian Journal of Animal Science, 14, 624–630.
Man, N.V., and Wiktorsson, H. 2002. Effect of molasses on nutritional quality of cassava and gliricidia tops silage. Asian–Australasian Journal of Animal Science, 15, 1294–1299.
Mapato, C., and Wanapat, M. 2018. Comparison of silage and hay of dwarf Napier grass (Pennisetum purpureum) fed to Thai native beef bulls. Tropical Animal Health and Production, 1–5. https://doi.org/10.1007/s11250-018-1582-y.
McGuire, D.L., Bohnert, D.W., Schauer, C.S., Falck, S.J., and Cooke, R.F. 2013. Daily and alternate day supplementation of urea or soy-bean meal to ruminants consuming low-quality cool-season forage: I—Effects on efficiency of nitrogen use and nutrient digestion. Livestock Science, 155, 205–213.
Merkel, R.C., Pond, K.R., Burns, J.C., and Fisher, D.S. 1999. Intake, digestibility and nitrogen utilization of three tropical tree legumes. II As protein supplement. Animal Feed Science and Technology, 82, 107–120.
Mikolayunas, S.C., Armentano, L.E., Thomas, D.L., and Berger, Y.M. 2009. Effect of protein degradability on milk production of dairy ewes. Journal of Dairy Science, 92, 4507–4513.
Morand, F.P., Fedele, V., Decandia, M., and Le Frileux, Y. 2007. Influence of farming and feeding systems on composition and quality of goat and sheep milk. Small Ruminant Research, 68, 20–34.
Moss, A.R., Jouany, J.P., and Newbold, J. 2000. Methane production by ruminants: its contribution to global warming. Annales de Zootechnie, 49, 231–253.
Newbold, C.J., Hassan, S.M., Wang, J., Ortega, M.E., and Wallace, R.A. 1997. Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria. British Journal of Nutrition, 78, 237–249.
NRC. 2001. Nutrient Requirements of Dairy Cattle. National Academy Press, Washington, DC., USA.
Oni, A.O., Sowande, O.S., Oni, O.O., Aderinboye, R.Y., Dele, P.A., Ojo, V.O.A., and Onwuka, C.F.I. 2014. Effect of additives on fermentation of cassava leaf silage and ruminal fluid of West African Dwarf goats. Archivos de Zootecnia, 243, 449–459.
Patra, A.K., and Sexana, J. 2011. Exploitation of dietary tannins to improve rumen metabolism and ruminant nutrition. Journal of the Science of Food and Agriculture, 91(1), 24–37.
Petterson, K., 1988. Ensiling of forages. Factors affecting silage fermentation and quality. Dissertation, Swedish University of Agricultural Science Report 179, Uppsala, Sweden.
Phesatcha, K., and Wanapat, M. 2016. Improvement of Leucaena silage nutritive value and in vitro ruminal fermentation by molasses and urea supplementation. Asian–Australasian Journal of Animal Science, 29(8), 1136–1144.
Pinho, E.Z., Costa, C., Arrigoni, M.D.B., Silveira, A.C., Padovani, C.R., and Pinho, S.Z. 2004. Fermentation and nutritive value of silage and hay made from the aerial part of cassava (Manihot esculenta, Crantz). Science Agriculture, 61, 364–370.
Ravindran V. 1991. Preparation of cassava leaf products and their use as animal feed. In: Roots, Tubers, Plantains and Bananas in Animal Feeding. Animal Production and Health Paper 95. FAO, Rome, 81–98.
Riaz, M.Q., Sudekun, K.H., Clauss, M., and Jayahegara, A. 2014. Voluntary feed intake and digestibility of focus domestic ruminant species as influenced by dietary constituents: A meta-analysis. Livestock Science, 162, 76–85.
Ribeiro, R.C.O., Villela, S.D.J., Valadares Filho, S.C., Santos, S.A., Ribeiro, K.G., Detmann, E., and Martins, P.G.M.A. 2015. Effects of roughage sources produced in a tropical environment on forage intake, and ruminal and microbial parameters. Journal of Animal Science, 93(5), 2363–2374.
Russell, J.B., and Rychlik J.L. 2001. Factors that alter rumen microbial ecology. Science, 292, 1119–22.
Samuel, M., Sagatheman, S., Thomas, J., and Mathen, G. 1997. An HPLC method for estimation of volatile fatty acids of ruminal fluid. Indian Journal of Animal Science, 67, 805.
SAS. (Statistical Analysis System), 2013. User’s Guide: Statistic, Version 9. 4th Edition. SAS Inst. Inc., Cary, NC.
Smith, P., Martino, D., Cai, Z., Gwary, D., Janzen, H., Kumar, P., McCarl, B., Ogle, S., O’Mara, F., Rice, C., Scholes, B., Sirotenko, O., Howden, M., McAllister, T., Pan, G., Romanenkov, V., Schneider, U., Towprayoon, S., Wattenbach, M., and Smith, J. 2008. Greenhouse gas mitigation in agriculture. Philosophical Transactions of the Royal Society B Biological Science, 363, 789–813.
Steel, R.G.D., and Torrie, J.H. 1980. Principles and Procedures of Statistics: A Biometrical Approach, 2nd ed. McGraw-Hill Book Company, New York, NY.
Tavendale, M.H., Meagher, L.P, Pacheco, D., Walker, N., Attwood, G.T., and Sivakumaran, S. 2005. Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Animal Feed Science and Technology, 123, 403–419.
Van Soest, P.J., Robertson, J.B., and Lewis, B.A. 1991. Methods for dietary fiber neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583–3597.
Wanapat, M., and Pimpa, O. 1999. Effect of ruminal NH3-N levels on ruminal fermentation, purine derivatives, digestibility and rice straw intake in swamp buffaloes. Asian–Australasian Journal of Animal Science, 12, 904–907.
Wanapat, M., Sundstol, F., and Garmo, T.H. 1985. A comparison of alkali treatment methods to improve the nutritive value of straw. I. Digestibility and metabolizability. Animal Feed Science and Technology, 12, 295–309.
Wanapat, M., Pimpa, O., Petlum, A., and Boontao, U. 1997. Cassava hay: a new strategic feed for ruminants during the dry season. Livestock Research and Rural Development, 9, 1–6.
Wanapat, M., Kang, S., Khejornsart, P., and Pilajun, R. 2013. Improvement of whole crop rice silage nutritive value and rumen degradability by molasses and urea supplementation. Tropical Animal Health and Production, 45(5), 1–5.
Wanapat, M., Kang, S., Khejornsart, P., Pilajun, R., and Wanapat, S. 2014a. Performance of tropical dairy cows fed whole crop rice silage with varying levels of concentrate. Tropical Animal Health and Production, 46(1), 185–189.
Wanapat, M., Gunun, P., Anantasook, N., and Kang, S. 2014b. Changes of rumen pH, fermentation and microbial population as influenced by different ratios of roughage (rice straw) to concentrate in dairy steers. The Journal of Agriculture Science (Cambridge), 152(4), 675–685.
Acknowledgements
Authors would like to express their most sincere thanks to Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand and the Thailand Research Fund (TRF) through the International Research Network (IRN) program (TRF-IRN 57W0002) and TRF-IRG 598001 for their kind support on research fund and facility.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Ethical guideline
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
Informed consent
Informed consent was obtained from all individual participants included in the study.
Rights and permissions
About this article
Cite this article
Wanapat, M., Phesatcha, K., Viennasay, B. et al. Strategic supplementation of cassava top silage to enhance rumen fermentation and milk production in lactating dairy cows in the tropics. Trop Anim Health Prod 50, 1539–1546 (2018). https://doi.org/10.1007/s11250-018-1593-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11250-018-1593-8