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Development of real-time PCR assay for the detection of Mycoplasma bovis

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Abstract

Mycoplasma bovis is one of the important bovine mycoplasma involved in economically important clinical conditions like respiratory diseases, otitis media, and mastitis. The present study was undertaken with the objective of developing a SYBR Green dye-based real-time PCR assay targeting uvrC gene for the diagnosis of M. bovis. The analytical sensitivity and specificity of the assay were evaluated. The test showed 103-fold more sensitivity than conventional PCR and detected down to 100 fg level of DNA. It was found to be specific, as no cross reactivity was shown with other related bacteria and Mycoplasma species. The developed assay was able to detect down to 40 copies of uvrC gene from spiked bovine milk samples. At present, this developed assay may be used as a valuable diagnostic tool for the detection of Mycoplasma bovis.

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Acknowledgments

The authors are thankful to the Director, IVRI, for providing necessary facilities to carry out the study.

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Authors and Affiliations

  1. Division of Bacteriology and Mycology, IVRI, Izatnagar, Bareilly, UP, India

    S. Behera, R. Rana, V. Rekha & T. R. Arun

  2. Division of Animal Biotechnology, IVRI, Izatnagar, Bareilly, UP, India

    P. K. Gupta, D. Kumar &  Sonal

  3. Division of Animal Reproduction, IVRI, Izatnagar, Bareilly, UP, India

    D. Jena

Authors
  1. S. Behera
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  2. R. Rana
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  3. P. K. Gupta
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  4. D. Kumar
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  5. Sonal
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  6. V. Rekha
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  7. T. R. Arun
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  8. D. Jena
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Correspondence to R. Rana.

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Behera, S., Rana, R., Gupta, P.K. et al. Development of real-time PCR assay for the detection of Mycoplasma bovis. Trop Anim Health Prod 50, 875–882 (2018). https://doi.org/10.1007/s11250-018-1510-1

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  • DOI: https://doi.org/10.1007/s11250-018-1510-1

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