Skip to main content
Log in

Pregnancy rate in water buffalo following fixed-time artificial insemination using new or used intravaginal devices with two progesterone concentrations

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

This study evaluated the pregnancy rate (PR) after timed artificial insemination (TAI) in water buffalo (Bubalus bubalis) during both non-breeding and breeding season, using either a new or reused intravaginal device (IVD) with two different progesterone concentrations. A total of 247 dairy buffalo cows were randomly assigned using a two-by-three factorial design and four replicates to the following groups: (1) new intravaginal device (IVD-New: DIB®, 1.0 g of P4, n = 51 or CIDR®, 1.38 g of P4, n = 55); (2) intravaginal device previously used once (9 days) (IVD-Used1x: DIB, n = 40 or CIDR, n = 51); or (3) intravaginal device previously used twice (18 days) (IVD-Used2x: DIB, n = 27 or CIDR, n = 23). On day 0, animals received the IVD plus 10.5 μg of buserelin acetate (GnRH) intramuscularly. On day 9, the devices were removed and 25 mg of PGF2α plus 500 IU of eCG was given intramuscularly. On day 11 (48 h after IVD withdrawal), animals received 10.5 μg of GnRH and were artificially inseminated 8–12 h later. Data were analyzed using Proc Logistic of SAS®. Animals that received IVD-New-DIB, had a significantly higher PR (62.7%; P = 0.0193) compared to animals that received IVD-New-CIDR (40%). Pregnancy rate was not negatively affected by reusing both types of IVD. Overall PR (new and reused devices) was higher (P = 0.0055) in the DIB group (62.7%) compared to the CIDR group (45%). In conclusion, PR was higher in buffaloes treated with devices containing 1.0 g of P4 (DIB®) compared to those receiving 1.38 g of P4 (CIDR®). Reusing the intravaginal devices did not affect negatively PR/TAI, suggesting that P4 concentrations within the TAI protocols in water buffaloes could be reduced, without impairing their fertility.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Alapati, A., Kapa, S.R., Jeepalyam, S., Rangappa, S.M. and Yemireddy, K.R., 2010. Development of the body condition score system in Murrah buffaloes: validation through ultrasonic assessment of body fat reserves, Journal of Veterinary Science, 11(1), 1–8

    Article  PubMed  PubMed Central  Google Scholar 

  • Baruselli P.S., Mucciolo R.G., Visintin J.A., Viana W.G., Arruda R.P., Madureira E.H., Oliveira C.A., Molero-Filho J.R.., 1997. Ovarian follicular dynamics during the estrous cycle in buffalo (Bubalus bubalis), Theriogenology, 47(8), 1531–1547

    Article  CAS  PubMed  Google Scholar 

  • Baruselli, P.S., Madureira, E.H., Barnabe, V.H., Barnabe, R.C. and Berber, R.C.A., 2003. Evaluation of synchronization of ovulation for fixed timed insemination in buffalo (Bubalus bubalis), Brazilian Journal of Veterinary Research and Animal Science, 40, 431–442. Accessed online: http://www.scielo.br/pdf/bjvras/v40n6/a07v40n6.pdf

  • Baruselli, P.S., SáFilho, M.F., Ferreira, R.M., Sales, J.N.S., Gimenes, L.U., Vieira, L.M., Mendanha, M.F. and Bó, G.A., 2012. Manipulation of follicle development to ensure optimal oocyte quality and conception rates in cattle, Reproduction in Domestic Animals, 47 (Suppl. 4), 134–141

    Article  PubMed  Google Scholar 

  • Carvalho, J.B.P., Carvalho, N.A.T., Reis, E.L., Nichi, M., Souza, A.H. and Baruselli, P.S., 2008. Effect of early luteolysis in progesterone-based timed AI protocols in Bos indicus, Bos indicus x Bos taurus, and Bos taurus heifers, Theriogenology, 69(2), 167–175

    Article  CAS  PubMed  Google Scholar 

  • Carvalho, N.A., Soares, J.G., Porto-Filho, R.M., Gimenes, L.U., Souza, D.C., Nichi, M., Sales, J.S. and Baruselli P.S., 2013. Equine chorionic gonadotropin improves the efficacy of a timed artificial insemination protocol in buffalo during the nonbreeding season. Theriogenology, 79(3), 423–428

    Article  CAS  PubMed  Google Scholar 

  • Carvalho, N.A., Soares, J.G., Souza, D.C., Vannucci, F.S., Amaral, R., Maio, J.R., Sales, J.N., SáFilho, M.F. and Baruselli P.S., 2014. Different circulating progesterone concentrations during synchronization of ovulation protocol did not affect ovarian follicular and pregnancy responses in seasonal anestrous buffalo cows, Theriogenology, 81 (3), 490–495

    Article  CAS  PubMed  Google Scholar 

  • Carvalho, N.A., Soares, J.G. and Baruselli, P.S., 2016. Strategies to overcome seasonal anestrus in water buffalo, Theriogenology, 86(1), 200–206

    Article  PubMed  Google Scholar 

  • Dadarwal, D., Mapletoft, R.J., Adams, G.P., Pfeifer, L.F.M., Creelman, C. and Singh, J., 2013. Effect of progesterone concentration and duration of proestrus on fertility in beef cattle after fixed-time artificial insemination, Theriogenology, 79 (5), 859–866

    Article  CAS  PubMed  Google Scholar 

  • De Rensis, F., Ronci, G., Guarneri, P., Nguyen, B.X., Presicce, G.A., Huszenicza, G. and Scaramuzzi, R.J., 2005. Conception rate after fixed time insemination following ovsynch protocol with and without progesterone supplementation in cyclic and non-cyclic Mediterranean Italian buffaloes (Bubalus bubalis), Theriogenology, 63(7), 1824–1831

    Article  PubMed  Google Scholar 

  • Echternkamp, S.E., Cushman, R.A. and Allan, M.F., 2009. Size of ovulatory follicles in cattle expressing multiple ovulations naturally and its influence on corpus luteum development and fertility, Journal of Animal Science, 87(11), 3556–3568

    Article  CAS  PubMed  Google Scholar 

  • Federation of Animal Science Societies (FASS). 2010. Guide for the Care and Use of Agricultural Animals in Agricultural Research and Teaching. Committees to revise the Guide for the Care and Use of Agricultural Animals in Research and Teaching, 3rd edition

  • Forde, N., Beltman, M.E., Lonergan P., Diskin, M., Roche, J.F., and Crowe M.A. 2011. Oestrous cycles in Bos taurus cattle, Animal Reproduction Science, 124(3–4), 163–169

    Article  CAS  PubMed  Google Scholar 

  • Long, S.T., Yoshida, C., and Nakao, T. 2009. Plasma progesterone profile in ovariectomized beef cows after intra-vaginal insertion of new, once-used or twice-used CIDR. Reproduction in Domestic Animals, 44(1):80–82

    Article  CAS  PubMed  Google Scholar 

  • Monteiro, B.M., de Souza, D.C., Vasconcellos, G.S., Corrêa, T.B., Vecchio, D., de SáFilho, M.F., Carvalho, N.A. and Baruselli, P.S., 2016. Ovarian responses of dairy buffalo cows to timed artificial insemination protocol, using new or used progesterone devices, during the breeding season (autumn-winter), Animal Science Journal, 87(1), 13–20

    Article  CAS  PubMed  Google Scholar 

  • Murugavel, K., Antoine, D., Raju, M.S. and López-Gatius, F., 2009. The effect of addition of equine chorionic gonadotropin to a progesterone-based estrous synchronization protocol in buffaloes (Bubalus bubalis) under tropical conditions, Theriogenology, 71(7), 1120–1126

    Article  CAS  PubMed  Google Scholar 

  • Neglia, G., Gasparrini, B., Di Palo, R., De Rosa, C., Zicarelli, L. and Campanile, G., 2003. Comparison of pregnancy rates with two estrus synchronization protocols in Italian Mediterranean Buffalo cows, Theriogenology, 60(1), 125–133

    Article  CAS  PubMed  Google Scholar 

  • Pandey, A.K., Dhaliwal, G.S., Ghuman, S.P. and Agarwal, S.K., 2011. Impact of pre-ovulatory follicle diameter on plasma estradiol, subsequent luteal profiles and conception rate in buffalo (Bubalus bubalis), Animal Reproduction Science, 123(3–4), 169–174

    Article  CAS  PubMed  Google Scholar 

  • Perera, B.M.A.O., 2008. Reproduction in domestic buffalo, Reproduction in Domestic Animals, 43 Suppl 2, 200–206

    Article  PubMed  Google Scholar 

  • Perera, B.M.A.O., 2011. Reproductive cycles of buffalo, Animal Reproduction Science, 124(3–4), 194–199

    Article  CAS  PubMed  Google Scholar 

  • Perry, G.A., Smith, M.F., Roberts, A.J., Macneil, M.D. and Geary, T.W., 2007. Relationship between size of the ovulatory follicle and pregnancy success in beef heifers, Journal of Animal Science, 85(3), 684–689

    Article  CAS  PubMed  Google Scholar 

  • Presicce, G.A., 2007. Reproduction in the water buffalo, Reproduction in Domestic Animals, 42 Suppl 2, 24–32, Review.

  • Presicce, G.A., Bella, A., Terzano, G.M., De Santis, G. and Senatore, E.M., 2005. Postpartum ovarian follicular dynamics in primiparous and pluriparous Mediterranean Italian buffaloes (Bubalus bubalis), Theriogenology, 63(5), 1430–1439

    Article  PubMed  Google Scholar 

  • SáFilho, M.F., Crespilho, A.M., Santos, J.E., Perry, G.A. and Baruselli, P.S., 2010. Ovarian follicle diameter at timed insemination and estrous response influence likelihood of ovulation and pregnancy after estrous synchronization with progesterone or progestin-based protocols in suckled Bos indicus cows, Animal Reproduction Science, 120(1–4), 23–30

    Article  Google Scholar 

  • Sales, J.N.S., Carvalho, J.B., Crepaldi, G.A., Soares, J.G., Girotto, R.W., Maio, J.R., Souza, J.C. and Baruselli, P.S., 2015. Effect of circulating progesterone concentration during synchronization for fixed-time artificial insemination on ovulation and fertility in Bos indicus (Nelore) beef cows, Theriogenology, 83(6), 1093–100

    Article  CAS  PubMed  Google Scholar 

  • SAS, SAS Institute, Inc., Cary, NC, 2001

  • Wiltbank, M.C., Sartori, R., Herlihy, M.M., Vasconcelos, J.L.M., Nascimento, A.B., Souza, A.H., Ayres, H., Cunha, A.P., Keskin, A., Guenther, J.N. and Gumen, A., 2011. Managing the dominant follicle in lactating dairy cows, Theriogenology, 76(9), 1568–1582

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are grateful to “Búfalos del Sur, C.A.” (BUFASUR) for providing all financial support and for allowing the use of their animals and facilities for this study.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to J. C. Gutiérrez-Añez.

Ethics declarations

Statement of animal rights

All animals were cared for in accordance with acceptable practices as for the Care and Use of Agricultural Animals in Agricultural Research and Teaching (FASS 2010).

Conflict of interest statement

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gutiérrez-Añez, J.C., Palomares, R.A., Jiménez-Pineda, J.R. et al. Pregnancy rate in water buffalo following fixed-time artificial insemination using new or used intravaginal devices with two progesterone concentrations. Trop Anim Health Prod 50, 629–634 (2018). https://doi.org/10.1007/s11250-017-1479-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11250-017-1479-1

Keywords

Navigation