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First report of molecular characterization and phylogenetic analysis of RoTat 1.2 VSG of Trypanosoma evansi from equine isolate

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Abstract

Rotat 1.2 variant surface glycoprotein (VSG) is considered to be an important VSG expressed in most of the isolates of Trypanosoma evansi. This makes the molecule an important candidate for both molecular- and serological-based detection of surra. There are ample reports of existence of this gene in isolates from cattle, buffalo, and camel across the world. Of late, there are reports of its absence from a fewer isolates of T. evansi of murine and wildlife origin. Search of literature revealed no reports from horses. The present communication presents the first report of molecular cloning and characterization of Rotat 1.2 VSG from horse isolate of T. evansi from semi-arid region of India. Alongside, the gene was compared with various other isolates across the world. Interestingly, the isolate was found to be closer to camel isolates from Egypt than the other known isolates from India and Kenya.

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Acknowledgements

The authors are thankful to Dean and Vice Chancellor DUVASU for the facilities provided and for the grant of Institutional project (185/DR/2015) for carrying out the research work. Authors also acknowledge the help of Dr. Harkirat Singh from GADVASU, Ludhiana during sequence analysis.

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Authors and Affiliations

  1. College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India

    Vikrant Sudan, Amit Kumar Jaiswal, Daya Shanker & Amit Kumar Verma

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  1. Vikrant Sudan
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  2. Amit Kumar Jaiswal
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  3. Daya Shanker
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  4. Amit Kumar Verma
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Correspondence to Vikrant Sudan.

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Sudan, V., Jaiswal, A.K., Shanker, D. et al. First report of molecular characterization and phylogenetic analysis of RoTat 1.2 VSG of Trypanosoma evansi from equine isolate. Trop Anim Health Prod 49, 1793–1796 (2017). https://doi.org/10.1007/s11250-017-1384-7

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