Abstract
Enzootic pneumonia (EP), often caused by Mycoplasma hyopneumoniae, occurs in Tibetan pigs between October and December in Western China. The aim of this study was to determine the prevalence of M. hyopneumoniae in Tibetan pig herds and also the prevalence of infection. M. hyopneumoniae was detected using enzyme-linked immunosorbent assay, polymerase chain reaction (PCR), and 16S rRNA sequencing. Twenty-nine inflammatory gross-lesions were observed in 155 lungs of slaughtered pigs. Invasion of focal lymphocytes was confirmed by paraffin sectioning and hematoxylin-eosin staining of lung sections. Bronchoalveolar lavage fluid (BALF) from slaughtered Tibetan pigs and nasal swabs from others were assayed using PCR. The prevalence of M. hyopneumoniae in Tibetan pig herds (via ELISA) was 20.48% (93/454) in 3 provinces (Sichuan, Tibet autonomous region, and Qinghai) between October and December of 2014. The difference in prevalence among animals in six different growing stages was statistically significant (P < 0.05). Anti-M. hyopneumoniae antibody was detected in breeding sows (45.83%; 22/48) and piglets (50%; 3/6). PCR and gel electrophoresis of BALF showed that 6.45% (10/155) of pigs were positive for M. hyopneumoniae. The presence of M. hyopneumoniae in serum was higher in piglets and breeding sows than in any other group. In conclusion, the results of this study showed that M. hyopneumoniae is prevalent among Tibetan pigs between October and December in Western China. To the authors’ knowledge, this is the first investigation of M. hyopneumoniae prevalence in Tibetan pigs of Western China using serological tests, PCR, and 16S rRNA sequencing.
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Acknowledgements
We would like to thank Lin Zhou (Nyingchi slaughterhouse, Tibet, China) for providing the blood and lung tissue samples.
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GQ and JKL designed the study. ZQ H, WT J, and XQ W collected samples from Sichuan and Qinghai, GQ and YPR performed the experiments. GQ and KL analyzed the data. GQ wrote the manuscript and JK L critically revised the manuscript. All authors read and approved the final version of manuscript.
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This work was supported by the National Key Research and Development Program of China (Project No. 2017YFD0502200) and mutual funds of Tibet Autonomous Science Department and Xizang Agriculture and Animal Husbandry College (2016ZR-NQ-4) entitled “Molecular epidemiology of Mycoplasma hyopneumoniae in Tibetan pigs.”
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Qiu, G., Rui, Y., Li, K. et al. Detection and phylogenetic analysis of Mycoplasma hyopneumoniae from Tibetan pigs in western China. Trop Anim Health Prod 49, 1545–1551 (2017). https://doi.org/10.1007/s11250-017-1365-x
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DOI: https://doi.org/10.1007/s11250-017-1365-x