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Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp. paratuberculosis disseminated infection in bovine liver

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Abstract

Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of MAP in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne’s disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of MAP in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne’s disease status. ELISA was performed to determine the MAP antibody activity in the serum. Acid-fast staining was performed on rectal scraping samples, and PCR was performed on rectal scraping and liver samples. PCR-positive liver samples were used for mycobacterial culture. Overall, the results of this study demonstrated that MAP can be detected and cultured from liver of slaughtered cattle and rapid diagnostic tests of Johne’s disease have limited value in detecting cattle with MAP infection in liver. These findings show that the presence of MAP in liver tissue may occur in cows with negative results for rapid diagnostic tests and vice versa. Hence, liver might represent another possible risk of human exposure to MAP. Given concerns about a potential zoonotic role for MAP, these results show the necessity to find new methods for detecting cattle with MAP disseminated infection.

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Acknowledgements

This study was supported by the research grant provided by the Shahid Chamran University of Ahvaz. The authors would like to thank Mr. A. Yousefvand for his kind assistance.

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Authors and Affiliations

  1. Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mehdi Zarei & Samaneh Tajbakhsh

  2. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Masoud Ghorbanpour

  3. Department of Bacteriology, Razi Vaccine and Serum Research Institute, Hessarak, Karaj, Iran

    Nader Mosavari

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  1. Mehdi Zarei
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  2. Masoud Ghorbanpour
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  3. Samaneh Tajbakhsh
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  4. Nader Mosavari
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Correspondence to Mehdi Zarei.

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Zarei, M., Ghorbanpour, M., Tajbakhsh, S. et al. Comparison of rapid diagnostic tests to detect Mycobacterium avium subsp. paratuberculosis disseminated infection in bovine liver. Trop Anim Health Prod 49, 1195–1200 (2017). https://doi.org/10.1007/s11250-017-1317-5

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  • DOI: https://doi.org/10.1007/s11250-017-1317-5

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