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Detection and identification of infectious bronchitis virus by RT-PCR in Iran

Tropical Animal Health and Production volume 48, pages 973–978 (2016)Cite this article

Abstract

Infectious bronchitis virus (IBV) causes severe diseases in poultry with significant economic consequences to the poultry industry in Iran. The aim of this study was the detection and identification of IBV by reverse transcription(RT)-PCR in Iran. Ten IB virus strains were detected by testing trachea, cecal tonsil, and kidney tissues collected from broiler and layer farms in Iran. In order to detect infectious bronchitis virus, an optimized RT-PCR was used. Primers targeting the conserved region of known IBV serotypes were used in the RT-PCR assay. Primers selectively detecting Massachusetts and 793/B type IB viruses were designed to amplify the S1 gene of the virus and used in the nested PCR test. Our findings indicate the circulation of at least three genotypes of IB viruses (Massachusetts, 793/B, and variant 2) among poultry flocks.

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Acknowledgments

The authors thank Mehdi Zarrin for his financial supports. We also thank Prof. Vilmos Palya for all his technical support. At the end, we thank Jafar Pazani and Nima Ghahremani for their commenting on the linguistic advice.

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Affiliations

  1. Symoorgh Avian Special Clinic, Tehran, Iran

    Alireza Homayounimehr, Ahmad Pakbin & Seyyedeh Mahsa Rastegar Fatemi

  2. Avian Disease and Scientific Research Department, Razi Vaccine and Serum Research Institute, Karaj, Iran

    Reza Momayyez

Authors
  1. Alireza Homayounimehr
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  2. Ahmad Pakbin
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  3. Reza Momayyez
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  4. Seyyedeh Mahsa Rastegar Fatemi
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Correspondence to Alireza Homayounimehr.

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Homayounimehr, A., Pakbin, A., Momayyez, R. et al. Detection and identification of infectious bronchitis virus by RT-PCR in Iran. Trop Anim Health Prod 48, 973–978 (2016). https://doi.org/10.1007/s11250-016-1040-7

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  • DOI: https://doi.org/10.1007/s11250-016-1040-7

Keywords

  • IBV in Iran
  • RT-PCR
  • 793/B
  • Massachusetts