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Determination of extended spectrum β-lactamases/AmpC β-lactamases and plasmid-mediated quinolone resistance in Escherichia coli isolates obtained from bovine carcasses in Mexico

Tropical Animal Health and Production volume 47, pages 975–981 (2015)Cite this article

Abstract

Food-borne bacterial infections have worldwide importance, and a great variety of antibiotic resistance mechanisms, mainly of the chromosome type, have rapidly developed. Antimicrobial resistance was determined in this study in terms of the presence of extended-spectrum β-lactamases (ESBLs), plasmid AmpC β-lactamases (pAmpC), and plasmid-mediated quinolone resistance (PMQR) from 155 Escherichia coli isolates obtained from bovine carcasses from two states in Mexico (states of Mexico and Jalisco). Isolates were challenged with β-lactam antimicrobials (ampicillin, ceftazidime, and cefotaxime) and quinolones (nalidixic acid and ciprofloxacin). The presence of the bla TEM, bla SHV, bla CTX-M, bla OXA , bla CMY, bla MOX, bla LAT, bla BIL, qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA genes was examined by PCR. Clonal relationship was determined using pulsed-field gel electrophoresis (PFGE). The highest resistance was found to be to nalidixic acid (64 %), followed by ampicillin (32 %), ciprofloxacin (10 %), and ceftazidime and cefotaxime (both 1.3 %). bla CMY (n = 1), bla TEM (n = 24), qnrB (n = 9), and qnrS (n = 7) genes were detected. PFGE analysis showed that the majority of isolates had a different genotypic profile. To our knowledge, this is the first report of the presence of the qnrB, qnrS, and bla CMY genes in E. coli isolated from bovine meat in Mexico.

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The authors declare that they have no conflict of interest.

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Affiliations

  1. Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Carretera Toluca-Atlacomulco, Km 15.5, 50200, Toluca, State of Mexico, Mexico

    Saúl Aguilar-Montes de Oca, Martín Talavera-Rojas & Edgardo Soriano-Vargas

  2. Departamento de Salud Pública, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Carretera a Nogales, Km. 15.5, 45110, Zapopan, Jalisco, Mexico

    Jeannette Barba-León

  3. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Carretera México-Toluca Km. 15.5, 05110, Palo Alto, D.F., Mexico

    Jesús Vazquez-Navarrete

Authors
  1. Saúl Aguilar-Montes de Oca
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  2. Martín Talavera-Rojas
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  3. Edgardo Soriano-Vargas
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  4. Jeannette Barba-León
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  5. Jesús Vazquez-Navarrete
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Correspondence to Martín Talavera-Rojas.

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Aguilar-Montes de Oca, S., Talavera-Rojas, M., Soriano-Vargas, E. et al. Determination of extended spectrum β-lactamases/AmpC β-lactamases and plasmid-mediated quinolone resistance in Escherichia coli isolates obtained from bovine carcasses in Mexico. Trop Anim Health Prod 47, 975–981 (2015). https://doi.org/10.1007/s11250-015-0818-3

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  • DOI: https://doi.org/10.1007/s11250-015-0818-3

Keywords

  • Escherichia coli
  • Antibiotic resistance
  • β-Lactams
  • Quinolones