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ESBL-producing Shiga-toxigenic E. coli (STEC) associated with piglet diarrhoea in India

Tropical Animal Health and Production volume 47, pages 377–381 (2015)Cite this article

Abstract

The present study was conducted to investigate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli carrying other virulence genes associated with piglet diarrhoea. Faecal samples from the piglets with history of diarrhoea were processed for isolation of E. coli and were tested for their ability of ESBL production. ESBL encoding genes and other virulence genes were detected by specific PCR, and amplicons were sequenced. Ability of transferring the ESBL genes between the enteric bacteria was tested in in vitro HGT. A total of 170 E. coli was isolated, of which 43 (25.29 %) were confirmed as ESBL producer by double disc synergy test (DDST). Altogether, 6.47 and 2.94 % isolates were positive for bla TEM and bla CTX-M-15 genes, respectively, of which 2.35 % isolates were positive for both the genes and only 0.6 % isolate was positive for the bla CTX-M gene alone. The resistance traits could not be transferred to the recipient host. Based on PCR, 2 (1.18 %) and 1 (0.59 %) isolates were recorded as Shiga-toxin-producing E. coli (STEC) and enteropathogenic E. coli (EPEC), respectively. Both the STEC (one isolate positive for stx 2 and another positive for stx 2 and hlyA) isolates belonging to serogroup O2 and NT were also positive for the bla CTX-M-15 gene. This is the first report of ESBL-producing E. coli isolate possessing the STEC gene associated with piglet diarrhoea.

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Acknowledgments

The authors are thankful to the Department of Biotechnology, Government of India, for providing fund, and to the Dean, College of Veterinary Sciences & Animal Husbandry, for providing all the facilities to conduct the present work.

Conflict of interest

The authors declare that they have no conflict of interest.

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Affiliations

  1. Department of Veterinary Microbiology, Central Agricultural University, Selesih, Aizawl, 796014, Mizoram, India

    Rajkumari Mandakini, Tapan Kumar Dutta, Santhalembi Chingtham, Parimal Roychoudhury, Abigail R. Pachauau & Rajesh Chandra

  2. Department of Veterinary Microbiology, West Bengal University of Animal & Fishery Sciences, 37, KB Sarani, Kolkata, 700037, West Bengal, India

    Indranil Samanta & Sidhartha Narayan Joardar

Authors
  1. Rajkumari Mandakini
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  2. Tapan Kumar Dutta
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  3. Santhalembi Chingtham
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  4. Parimal Roychoudhury
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  5. Indranil Samanta
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  6. Sidhartha Narayan Joardar
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  7. Abigail R. Pachauau
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  8. Rajesh Chandra
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Correspondence to Tapan Kumar Dutta.

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Mandakini, R., Dutta, T.K., Chingtham, S. et al. ESBL-producing Shiga-toxigenic E. coli (STEC) associated with piglet diarrhoea in India. Trop Anim Health Prod 47, 377–381 (2015). https://doi.org/10.1007/s11250-014-0731-1

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  • DOI: https://doi.org/10.1007/s11250-014-0731-1

Keywords

  • ESBLs
  • STEC
  • Pig
  • India