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HSP70 as a marker of heat and humidity stress in Tarai buffalo

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Abstract

Heat and humidity stress is a constant challenge to buffalo rearing under tropical climatic conditions. Heat shock proteins (HSPs) constitute a ubiquitous class of highly conserved proteins that contribute to cell survival during different conditions of stress. The present study was carried out in Tarai buffaloes to study the expression of HSP70 in their peripheral blood mononuclear cells during different seasons and establish it as a marker of heat and humidity stress in buffaloes. Blood samples were collected from each healthy, non-lactating and non-pregnant buffalo above 2 years of age, once in the month of January (temperature-humidity index (THI) < 72) and in the month of May (THI > 72). Blood samples were also collected during October (THI = 72) to be used as calibrator/control. Real-time PCR was used to profile the HSP70 gene expression in the peripheral blood mononuclear cells (PBMCs). The relative expression values of HSP70 in Tarai buffalo was found to be significantly higher (P < 0.05) during summer season (2.37 ± 0.12) as compared to winter season (0.29 ± 0.04). The expression positively correlated with changes in physiological parameters like respiration rate (RR), pulse rate (PR) and rectal temperature (RT). In conclusion, it can be said that RR and HSP70 may act as characteristic physiological and cellular markers of heat and humidity stress in buffaloes.

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Acknowledgments

Funding support received from the GBPUAT, Pantnagar, Uttarakhand is gratefully acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Veterinary Physiology & Climatology, College of Veterinary & Animal Sciences, GBPUAT, Pantnagar, 263145, Uttarakhand, India

    Rao Manjari, Mrigakshi Yadav, Sarveshwa Uniyal & Sunil Kumar Rastogi

  2. Animal Physiology Division, NIANP, Bangalore, 560030, India

    Veerasamy Sejian

  3. Department of Veterinary Physiology, NTR CVSc, Gannavaram, 521102, India

    Iqbal Hyder

  4. Division of Veterinary Physiology, IVRI, Izzatnagar, 521102, India

    Kandasamy Ramesh

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  1. Rao Manjari
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  2. Mrigakshi Yadav
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  3. Kandasamy Ramesh
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  4. Sarveshwa Uniyal
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  5. Sunil Kumar Rastogi
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  6. Veerasamy Sejian
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  7. Iqbal Hyder
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Correspondence to Iqbal Hyder.

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Manjari, R., Yadav, M., Ramesh, K. et al. HSP70 as a marker of heat and humidity stress in Tarai buffalo. Trop Anim Health Prod 47, 111–116 (2015). https://doi.org/10.1007/s11250-014-0692-4

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  • DOI: https://doi.org/10.1007/s11250-014-0692-4

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