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Identification and molecular analysis of infectious bursal disease in broiler farms in the Kurdistan Regional Government of Iraq

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Abstract

The present study was undertaken to characterize field isolates of infectious bursal disease virus (IBDV). The identification was done using reverse transcription-polymerase chain reaction (RT-PCR) and partial sequencing of the VP2 gene. Pooled bursal samples were collected from commercial broiler farms located in the Kurdistan Regional Government (KRG) of Iraq. The genetic material of the IBDV was detected in 10 out of 29 field samples. Sequences of the hypervariable VP2 region were determined for 10 of these viruses. Molecular analysis of the VP2 gene of five IBDVs showed amino acid sequences consistent with the very virulent (vv) IBDV. Two samples were identified as classic vaccine viruses, and three samples were classic vaccine viruses that appear to have mutated during replication in the field. Phylogenetic analysis showed that all five field IBDV strains of the present study were closely related to each other. On the basis of nucleotide sequencing and phylogenetic analysis, it is very likely that IBD-causing viruses in this part of Iraq are of the very virulent type. These IBDVs appear to be evolving relative to their type strains.

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Acknowledgments

We thank the Ohio Agricultural Research and Development Center, Ohio University, USA, for partly funding this study. We also would like to thank the Barash Company in Erbil, KRG, for helping collect the samples from the broiler farms.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Animal Health Department, General Directorate of Animal Wealth and Veterinary, Ministry of Agriculture and Water Resources, Kurdistan Regional Government, Erbil, Iraq

    Oumed Gerjis M. Amin

  2. Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 25 Edgington Hall, 1680 Madison Ave., Wooster, OH, 44691, USA

    Daral J. Jackwood

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  1. Oumed Gerjis M. Amin
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Correspondence to Oumed Gerjis M. Amin.

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Amin, O.G.M., Jackwood, D.J. Identification and molecular analysis of infectious bursal disease in broiler farms in the Kurdistan Regional Government of Iraq. Trop Anim Health Prod 46, 1297–1301 (2014). https://doi.org/10.1007/s11250-014-0643-0

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