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In vivo effect of selected medicinal plants against gastrointestinal nematodes of sheep

Tropical Animal Health and Production volume 46, pages 411–417 (2014)Cite this article

Abstract

Nematode resistance to anthelmintic drugs affects small ruminant production globally. This study evaluated in vivo effects of five plant extracts as alternative nematode treatments. Animals were divided into six groups. Each group was randomly assigned a treatment: Abamectin and Praziquantel (CAP) (positive Control), ethanol extracts of Ananas comosus (AC), Aloe ferox (AF), Allium sativum (AS), Lespedeza cuneata (LC) and Warburgia salutaris (WS). These were applied as an oral dose (100 mg kg−1 BW), one dose per week per sheep for 42 days (Phase 1). From day 42 sheep were orally dosed for 3 consecutive days with the same treatments in the same groups (Phase 2). Rectal faecal samples were taken every 7 days up to day 63. Eggs per gram of faeces were counted in individual rectal samples, L3 larval stage was counted in faecal cultures, with four replicates per group. For plant extracts, EPG decreased (P < 0.001) with time and efficacy of plant increased (P < 0.001) with time. Ananas comosus and L. cuneata treatments had the highest efficacies of 58 % and 61 %, respectively, in Phase 1; and 77 % and 81 %, respectively, in Phase 2. Continuous treatment with these plants could further reduce nematode parasites and improve host health.

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References

  • Ademola, I.O., Fagbemi, B.O. and Idowu, S.O., 2004. Evaluation of the anthelmintic activity of Khaya senegalensis extract against gastrointestinal nematodes of sheep: in vitro and in vivo studies. Veterinary Parasitology, 122, 151–164

    CAS  PubMed  Article  Google Scholar 

  • Ahmed, M., 2010. Gastrointestinal (nematode) Infections in small ruminants: epidemiology, anthelmintic efficacy and the effect of wattle tannins. MSc thesis, University of KwaZulu-Natal, Pietermaritzburg, South Africa

  • Akhtar, M., 1999. Biological control of plant-parasitic nematodes in pigeonpea field crops using neem-based products and manurial treatments. Applied Soil Ecology, 12, 191–195

    Article  Google Scholar 

  • Akhtar, M. and Malik, A. 2000. Roles of organic soil amendments and soil organisms in the biological control of plant-parasitic nematodes: a review. Bioresource Technology, 74, 35–47

    CAS  Article  Google Scholar 

  • Ameh, S.J., Obodozie, O.O., Abubakar, M.S. and Garba, M., 2010. Current phytotherapy–an inter-regional perspective on policy, research and development of herbal medicine. Medicinal Plants Research, 4, 1508–1516

    Google Scholar 

  • Amin, M.R., Mostofa, M., Awal, M.A. and Sultana, M.A., 2008. Effects of garlic, turmeric and betel leaf against gastrointestinal nematodes in cattle. Bangladesh Veterinary Medicine, 6, 115–119

    Google Scholar 

  • Aremu, A.O., 2009. Pharmacology and phytochemistry of South African plants used as anthelmintics. MSc thesis, University of KwaZulu-Natal, Pietermaritzburg, South Africa

  • Baloyi, M.A., Laing, M.D. and Yobo, K.S., 2012. Use of mixed cultures of biocontrol agents to control sheep nematodes. Veterinary Parasitology, 184, 367–370

    CAS  PubMed  Article  Google Scholar 

  • Buttle, D.J., Behnke, J.M., Bartley, Y., Elsheikha, H.M., Bartley, D.J., Garnett, M.C., Donnan, A.A., Jackson, F., Lowe, A. and Duce, I.R., 2011. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus. Parasites and Vectors, 4, 36 available on line: http://www.parasitesandvectors.com/content/4/1/36 accessed date: 01/04/2011

  • Cringoli, G., Veneziano, V., Rinaldi, L., Sauve, C., Rubino, R., Fedele, V. and Cabaret, J., 2007. Resistance of trichostrongyles to benzimidazoles in Italy: a first report in a goat farm with multiple and repeated introductions. Parasitology Research, 101, 577–581

    CAS  PubMed  Article  Google Scholar 

  • Eguale, T., Tilahun, G., Debella, A., Feleke, A. and Makonnen, E., 2007. In vitro and in vivo anthelmintic activity of crude extracts of Coriandrum sativum against Haemonchus contortus. Journal of Ethnopharmacology, 110, 428–433

    CAS  PubMed  Article  Google Scholar 

  • Ferri, N., Yokoyama, K., Sadilek, M., Paoletti, R., Apitz-Castro, R., Gelb, M.H. and Corsini, A., 2003. Ajoene, a garlic compound, inhibits protein prenylation and arterial smooth muscle cell proliferation. Journal of Pharmacology, 138, 811–818.

    CAS  Google Scholar 

  • Geary, T.G., Sangster, N.C. and Thompson, D.P., 1999. Frontiers in anthelmintic pharmacology. Veterinary Parasitology, 84, 275–295

    CAS  PubMed  Article  Google Scholar 

  • Gillian, S., Behnke, J.M., Buttle, D.J. and Duce, L.R., 2004. Natural plant cysteine proteinases as anthelmintics. Trends in Parasitology, 20, 322–327

    Article  Google Scholar 

  • Hansen, J. and Perry, B. 1994. The epidemiology, diagnosis and control of helminth parasites of ruminants (Handbook). International Laboratory for Research on Animal Diseases Press, Nairobi, Kenya. Electronic version available in www.fao.org/wairdocs/ILRI/x5492E/x5492E00.HTM accessed on 11/4/2008

  • Hördegen, P., Cabaret, J., Hertzberh, H., Langhans, W. and Maurer, V., 2006. In vitro screening of six anthelmintic plant products against larval Haemonchus contortus with a modified methyl-thiazolyl-tetrazolium reduction assay. Ethnopharmacology, 108, 85–89

    PubMed  Article  Google Scholar 

  • Iqbal, Z., Nadeem, Q.K., Khan, M.N., Akhtar, M.S. and Waraich, F.N., 2001. In vitro anthelmintic activity of Allium sativum, Zingiber officinale, Curcurbita mexicana and Ficus religiosa. International Agriculture and Biology, 3, 454–457

    Google Scholar 

  • Leathwick, D.M., Pomroy, W.E. and Heath, A.C., 2001. Anthelmintic resistance in New Zealand. New Zealand Veterinary Journal, 49, 227–235.

    CAS  PubMed  Article  Google Scholar 

  • Mabusela, W.T., Stephen, A.M. and Botha, M.C., 1990. Carbohydrate polymers from Aloe ferox leaves. Phytochemistry, 29, 3555–3558

    CAS  Article  Google Scholar 

  • Maphosa, V., Masika, P.J., Bizimenyera, E.S. and Eloff, J.N., 2010. In vitro anthelmintic activity of crude aqueous extracts of Aloe ferox, Leonotis leonurus and Elephantorrhiza elephantina against Haemonchus contortus. Tropical Animal Health and Production, 42, 301–307

    PubMed  Article  Google Scholar 

  • McCorkle, M., Mathias, E. and Schillhorn van Veen, T.W., 1996. Ethnoveterinary research and development, studies in indigenous knowledge and development. Intermediate Technology Publications, Southampton Row, London, 1–23

    Google Scholar 

  • McGaw, L.J. and Eloff, J.N., 2008. Ethnoveterinary use of Southern African plants and scientific evaluation of their medicinal properties. Ethnopharmacology, 119, 686–699

    PubMed  Article  Google Scholar 

  • Min, B.R., Barry, T.N., Attwood, G.T. and McNabb, W.C., 2003. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review. Animal Feed Science and Technology, 106, 3–19

    CAS  Article  Google Scholar 

  • Min, B.R., Pomroy, W.E., Hart, S.P. and Sahlu, T., 2004. The effect of short-term consumption of a forage containing condensed tannins on gastrointestinal nematode parasite infections in grazing goats. Small Ruminant Research, 51, 279–283

    Article  Google Scholar 

  • Minho, A.P., Bueno, I.C., Louvandini, H., Jackson, F., Gennari, S.M. and Abdalla, A.L., 2008. Effect of Acacia molissima tannin extract on the control of gastrointestinal parasites in sheep. Animal Feed Science and Technology, 147, 172–181

    CAS  Article  Google Scholar 

  • Mohanlall, V. and Odhav, B., 2009. Furans and furanones with antimycotoxigenic activity isolated from Warburgia salutaris (Canellaceae). Medicinal Plants Research, 3, 231–240

    CAS  Google Scholar 

  • Molan, A.L., Waghorn, G.C., Min, B.R. and McNabb, W.C., 2000. The effect of condensed tannins from seven herbages on Trichostrongylus colubriformis larval migration in vitro. Folia Parasitological, 47, 39–44

    CAS  Article  Google Scholar 

  • Olayiwola, A., 1993. Nature’s medicinal bounty, don’t throw it away. World Health Forum, 14, 391–395

    Google Scholar 

  • Overend, D.J., Phillips, M.L., Poultion, A.L. and Foster, C., 1994. Anthelmintic resistance in Australian sheep nematode populations. Australian Veterinary Journal, 71, 117–121

    CAS  PubMed  Article  Google Scholar 

  • SAS., 2000. Statistical analysis system user’s guide (Version 8). SAS Institute Inc., SAS Campus Drive, Cary, N.C., USA

    Google Scholar 

  • Schnyder, M., Torgerson, P.R., Schönmann, M., Kohler, L. and Hertzberg, H., 2005. Multiple anthelmintic resistance in Haemonchus contortus isolated from South African Boer goats in Switzerland. Veterinary Parasitology, 128, 285–290

    CAS  PubMed  Article  Google Scholar 

  • Singh, T.U., Kumar, D., Tandan, S.K. and Mishra, S.K., 2009. Inhibitory effect of essential oils of Allium sativum and Piper longum on spontaneous muscular activity of liver fluke, Fasciola gigantica. Experimental Parasitology, 123, 302–308

    CAS  PubMed  Article  Google Scholar 

  • Southcott, W.H., Major, G.W. and Barger, I.A., 1976. Seasonal pasture contamination and availability of nematodes for grazing sheep. Agriculture Research, 27, 277–286

    Google Scholar 

  • Stepek, G., Lowe, A.E., Buttle, D.J., Duce, I.R. and Behnke, J.M., 2006. In vitro and in vivo anthelmintic efficacy of plant cysteine proteinases against the rodent gastrointestinal nematode, Thirchuris muris. Parasitology, 132, 681–689

    CAS  PubMed  Article  Google Scholar 

  • Sujon, M.A., Mostofa, M., Jahan, M.S., Das, A.R. and Rob, S., 2008. Studies on medicinal plants against gastrointestinal nematodes of goats. Bangladesh Veterinary Medicine, 6, 179–183

    Google Scholar 

  • Tembely, S., Lahlou-Kassi, A., Rege, J.E., Sovani, S., Diedhiou, M.L. and Bake, R.L., 1997. The epidemiology of nematode infections in sheep in a cool tropical environment. Veterinary Parasitology, 70, 129–141

    CAS  PubMed  Article  Google Scholar 

  • Van Wyk, J.A., Cabaret, J. and Michael, L.M., 2004. Morphological identification of nematode larvae of small ruminants and cattle simplified. Veterinary Parasitology, 119, 277–306

    PubMed  Article  Google Scholar 

  • Waller, P.J., Knox, M.R. and Faedo, M., 2001. The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: Feeding and block studies with Duddingtonia flagrans. Veterinary Parasitology, 102, 321–330

    CAS  PubMed  Article  Google Scholar 

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Acknowledgements

The research fund was provided by the National Research Foundation, South Africa.

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Authors and Affiliations

  1. Department of Animal and Poultry Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville, 3209, South Africa

    Mawahib Ahmed & Ignatius V. Nsahlai

  2. Discipline of Plant Pathology, University of KwaZulu-Natal, Private Bag X01, Scottsville, 3209, South Africa

    Mark D. Laing

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  1. Mawahib Ahmed
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  3. Ignatius V. Nsahlai
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Correspondence to Mawahib Ahmed.

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Ahmed, M., Laing, M.D. & Nsahlai, I.V. In vivo effect of selected medicinal plants against gastrointestinal nematodes of sheep. Trop Anim Health Prod 46, 411–417 (2014). https://doi.org/10.1007/s11250-013-0506-0

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Keywords

  • Biological control
  • Plant extracts
  • Nematodes
  • Ruminants